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Citations for Promega :
101 - 150 of 699 citations for Yellow Fever virus lysate Asibi strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Molecular Biology 2021Quote: 500 µl rabbit reticulocyte lysate obtained from Promega (L4151, untreated) was supplemented with 1 µl 12.5 mM Hemin (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... using the TnT Quick-Coupled reticulocyte lysate system (Promega #L1170) and 35S-Methionine (Perkin Elmer #NEG709A001MC) ...
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bioRxiv - Cell Biology 2019Quote: ... and lysates were analyzed using a GloMax® Luminometer (Promega). Transfection and expression efficiencies were normalized to the activity of β-galactosidase activities.
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Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entrybioRxiv - Biochemistry 2021Quote: ... The cell lysates were firstly digested with trypsin (Promega, USA) by the in-solution digestion method22 ...
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
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bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...
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bioRxiv - Molecular Biology 2022Quote: A rabbit reticulocyte lysate in vitro translation kit (Promega, L4960) was coupled to in vitro transcription with the supplementation of MgCl2 ...
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bioRxiv - Microbiology 2023Quote: Cell lysate was prepared by using passive lysis buffer (Promega). Firefly and renilla luciferase activities (Fluc and Rluc ...
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bioRxiv - Biochemistry 2023Quote: ... with the T7 TNT Coupled Reticulocyte Lysate System (Promega, L1170). Each substrate was amplified with primers by PCR (using template plasmids from the hORFeome collection or commercially available plasmids ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... Lysates were mixed with Firefly luciferase assay reagent (E1500 Promega) as per manufacturer’s protocol and scanned for luminescence in Tecan scanner.
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bioRxiv - Microbiology 2023Quote: ... lactis strains were extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). Real-time PCR was done using a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Microbiology 2024Quote: DNA of strain JS3066 was extracted with a Wizard Genomic Purification Kit (Promega, USA). The genome sequencing and assembly was done by the BGI Medical Examination Co. ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus -specific PCR products were cloned into pGEM-T Easy Vector (A1360, Promega Co., Madison, WI, USA) and used to transform E ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... and translated using the nuclease-treated rabbit reticulocyte lysate (RRL; Promega). Typical reactions were comprised of 75% v/v RRL ...
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bioRxiv - Cell Biology 2021Quote: ... the mRNA was translated in a Flexi rabbit reticulocyte lysate (Promega) containing 35S methionine/cysteine (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were analyzed using a Dual-Luciferase Reporter Assay System (Promega) with a Tecan Microplate Reader (Tecan Infinite M1000) ...
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bioRxiv - Molecular Biology 2020Quote: ... which was synthesized in vitro with reticulocyte lysate systems (Promega, L5020), for 2 h at room temperature in binding buffer (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... and the lysate was treated with 20U/ml RNase A (Promega) and 60U/ml RQ1 DNase (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... The acquired mRNA was then translated in rabbit reticulocyte lysates (Promega) containing 35S- labelled Met and Cys ...
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bioRxiv - Immunology 2020Quote: ... Dual luciferase activity in the total cell lysates was quantified (Promega).
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were treated with RNAse free DNAse (Promega Corp) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA transcripts were then translated in rabbit reticulocyte lysate (RRL) (Promega) in the presence of L-35S-methionine (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro translated with a Rabbit Reticulocyte Lysate system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were then subjected to Dual-Luciferase Reporter assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl reactions contained 60% nuclease-treated rabbit reticulocyte lysate (Promega), 20 μM amino acid mixture minus leucine ...
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bioRxiv - Microbiology 2021Quote: ... coli LPS strain W3110 (kindly provided by Robert Ernst) using FuGENE HD transfection reagent (Promega) for 6 hours.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli strains were performed using the PureYield™ Plasmid Miniprep System (Promega Corp., Madison, WI) according to manufacturer protocols ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...