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Citations for Promega :
251 - 300 of 1551 citations for Yellow Fever Virus Envelope Protein Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... Human GSK-3β was purchased from Promega (V1991).
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bioRxiv - Genomics 2020Quote: ... and human gDNA (Promega, San Luis Obispo, CA) was added to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Biochemistry 2021Quote: ... and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Mass Spec-Compatible Human Protein Extracts, Cat# V6951) and digested yeast protein extract (Mass Spec-Compatible Yeast Protein Extracts, Cat# V7461) were from Promega, commercial human plasma samples (Human Source Plasma ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were purified by using MagneHis™ Protein Purification System (Promega, USA). The peptides of the transporters PDR6 ...
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bioRxiv - Biophysics 2022Quote: ... Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821 ...
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bioRxiv - Neuroscience 2021Quote: ... reverse mutant primer oIMR1437 5′-TCC ACC TAG CCT GCC TGT AC-3′) with 1U GoTaq polymerase (Promega, Madison, USA), 1X green GoTaq buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei were isolated by resuspending cell pellets in 200 μL Dig-wash buffer (CUT&Tag wash buffer supplemented with 0.05% digitonin (Promega, G9441) and 0.05% IGEPAL CA-630 (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... cDNAs were obtained from Origene in pCMV6 vector that also incorporates C-terminal Myc and FLAG tags (Myc-FLAG) or subcloned into a pHTC (Promega)-derived vector that expresses C-terminal HaloTag (41) ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of EIF3A (or mutants lacking U/TAG motif) were cloned downstream of the Renilla luciferase in the XhoI and NotI sites of psiCHECK2 vector (Promega, Madison ...
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bioRxiv - Microbiology 2022Quote: Templates for in vitro transcription of gene-specific 300-500 nt dsRNA and DENV2 EMSA probes were generated by PCR introducing a T7 promoter sequence or a universal tag at both 5’ and 3’ ends using the GoTaq Flexi DNA Polymerase (Promega). If present ...
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bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
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bioRxiv - Cell Biology 2020Quote: DRGs neurons infected with lentivirus expressing Halo-SFPQ were grown in XonaChip microfluidic chambers and were labeled with tetramethyl Rhodamine (TMR) Halo Tag ligand (Promega) according to the manufacturer’s instructions with modifications ...
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bioRxiv - Microbiology 2021Quote: ... where the multiple cloning site had been replaced by a synthetic construct (Genewiz) composed of a 5’ HiBiT tag (Promega), a new multiple cloning site and 3’ 3xHA tag ...
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bioRxiv - Bioengineering 2019Quote: ... after removal of its original signal peptide and was further fused to Halo-tag derived from HaloTag Control Vector (Promega) at the C-terminus ...
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bioRxiv - Biophysics 2019Quote: ... Cells were stained with SiR-dye (kindly provided by Kai Johnson, EFPL, Lausanne, Switzerland) shortly before imaging according to the Halo-tag protocol (Promega).
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bioRxiv - Cell Biology 2020Quote: ... Some cells at this stage were seeded onto coverslips and stable transfection of desired plasmids was confirmed by using specific fluorescent ligands to Halo-tag (TMR, Promega). The cells seeded in T75 flasks were allowed to grow for further 3-4 weeks in complete media with G418 replaced twice a week ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Molecular Biology 2021Quote: ... A protein standard (Promega) was added to the non-tagged cells as a known reference of protein concentration to luminescence output ...
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bioRxiv - Microbiology 2022Quote: ... Protein Precipitation Solution (Promega) was added to the lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... HaloTag Standard Protein (Promega) was labelled with 10 fold molar excess (5 µM ...
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bioRxiv - Genetics 2023Quote: ... Protein Precipitation Solution (Promega) was added to the lysed mixture ...
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bioRxiv - Microbiology 2023Quote: ... Protein precipitation solution (Promega) was added and samples were vigorously mixed ...
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA synthesis was carried out using 2 ug of total RNA and reverse transcription was performed using an oligo (dT) 15 primer and Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA). qPCR was carried out using SYBR GREEN PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... oligo deoxythymidylic acid (oligo-dT) primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Cancer Biology 2021Quote: ... and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study ...