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Citations for Promega :
101 - 150 of 3367 citations for WesternBright MCF IR fluorescent Western blotting kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells between passage 10 – 30 grown in 40-mm wells were transiently transfected at 30 – 50% confluency with plasmids encoding for TRPM2DR and green fluorescent protein (GFP) using FuGene6 (Promega). Transfected cells were subcultured onto laminin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2019Quote: ... Cells were transfected with target channel-cloned pBiEX vectors and enhanced green fluorescent protein (EGFP)-cloned pBiEX vectors using Fugene HD transfection reagent (Promega). The channel-cloned vector (2 μg ...
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bioRxiv - Biochemistry 2019Quote: ... expression and purification described in Supporting Information) with (experimental) and without (control) 100 nM HaloTag NanoBRET 618 fluorescent ligand (Promega) was combined with diacetylated or triacetylated histone peptide concentrations ranging from 10 nM to 100 µM in white ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Biophysics 2022Quote: ... 60 µm wide (diameter at 1/e2 peak intensity) in the sample plane as characterised using a sample of immobilised standard fluorescent microbeads (Promega). The source power was 30 mW ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent signal (excitation at 475 nm, emission at 500-550 nm) was measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Microbiology 2022Quote: ... both OD600nm and fluorescent signal (excitation at 475 nm, emission at 500-550 nm) were measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Cell Biology 2020Quote: ... Some cells at this stage were seeded onto coverslips and stable transfection of desired plasmids was confirmed by using specific fluorescent ligands to Halo-tag (TMR, Promega). The cells seeded in T75 flasks were allowed to grow for further 3-4 weeks in complete media with G418 replaced twice a week ...
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bioRxiv - Immunology 2020Quote: The fluorescent fusion assay was adapted to allow quantitative measurement of cell-cell fusion by leveraging the NanoBiT complementation system (Promega). Donor HEK293 cells were transfected with full-length S and the 11S subunit in 96-well white flat bottom TC-treated microtest assay plates ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Developmental Biology 2024Quote: pCS2+ expression vectors carrying membrane-localized fluorescent CAAX-GFP (mGFP) or Utrophin-RFP (utr-RFP) were linearized using restriction enzymes (Promega) for two hours at 37°C and precipitated at -20°C overnight in 70% ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Biochemistry 2022Quote: ... Colonies that had either a homozygous or heterozygous insertion were further screened with a western blot using Anti-HaloTag (Promega; G921A) and compared with the parental cell line as a negative control for final validation.
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bioRxiv - Microbiology 2019Quote: ... then the GFP expression level was observed under a fluorescent microscope and luciferase activity was measured using a Renilla Luciferase Assay System (Promega, Fitchburg, WI). The detailed methodology is described in the luciferase assay section.
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.
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bioRxiv - Microbiology 2020Quote: NCIMB8209 DNA was isolated using a commercial kit (Wizard Genomic DNA purification kit, Promega), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... column purification kits or Maxwell 16 LEV simplyRNA Purification Kits (Promega, Madison, WI, USA) and prepared cDNA libraries using TruSeq RNA Library Prep Kit (Illumina ...
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bioRxiv - Zoology 2022Quote: DNA was extracted with several different kits: the Wizard Genomic DNA Purification kit (Promega), the Qiagen Blood & Tissue kit ...
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bioRxiv - Microbiology 2019Quote: The luciferase assay kit (Promega) was used to test the expression and regulation performance of the selected transactivator-positive clones ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase3 / 7 Kit (Promega company).
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bioRxiv - Biochemistry 2020Quote: ... CellTiter-Glo 2 kit (Promega) using manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Access RT-PCR kit (Promega) was used for characterization of recombinant influenza viruses.
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bioRxiv - Microbiology 2019Quote: ... Wizard gDNA Purification Kit (Promega) was used to isolate and purify gDNA from CEM-SS cells.
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bioRxiv - Cancer Biology 2019Quote: ... The dual luciferase kit (Promega) was used to measure activity using a luminometer ...
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bioRxiv - Plant Biology 2020Quote: ... A Dual Luciferase kit (Promega) was used to detect reporter activity ...
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bioRxiv - Molecular Biology 2020Quote: ... Random Primers Kit (Promega, A2800) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaussia luciferase kit from Promega was used for detecting secreted Gaussia luciferase signal as per manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... the commercial AMPGlo kit (Promega) was used according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2021Quote: ... chromosomal isolation kit (Promega (USA)) ...
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bioRxiv - Plant Biology 2020Quote: ... Oligo dT kit (Promega, USA) according to the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... GoTaq®qPCR kit (Promega) was used for qPCR analysis ...
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bioRxiv - Genomics 2021Quote: ... PureYield plasmid miniprep kits (Promega), or QIAprep Spin Miniprep kits ...
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bioRxiv - Cell Biology 2022Quote: ... The MTT assay kit (Promega) was used according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: GoTaq DNA polymerase kits (Promega) were used for PCR reactions ...
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bioRxiv - Genomics 2023Quote: ... the Tunnel staining kit (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... sequencing reaction (fmol kit, Promega) using the same end labeled primer was run to identify the exact position of RT stops.
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bioRxiv - Neuroscience 2023Quote: ... Oligo (dT) kit (Promega, #A2791) to reverse transcribe the RNA into cDNA following the manufacturer protocol.
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bioRxiv - Microbiology 2023Quote: ... Reverse Transcription Kit (Promega, USA); IBV nucleic acid amplification fluorescence detection kit (Shanghai Furex Medical Scientific and Technological Development Co. ...
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bioRxiv - Cancer Biology 2024Quote: ... Core Kit (Promega, Wisconsin, USA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...