Labshake search
Citations for Promega :
1 - 50 of 917 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... and 90 μL Fμgene HD (Promega E2312) were incubated for 10 min at r.t ...
-
bioRxiv - Biophysics 2023Quote: ... and 90 μL Fμgene HD (Promega E2312) were incubated for 10 min at r.t ...
-
bioRxiv - Microbiology 2022Quote: ... RNasin Plus RNase Inhibitor 90 U (Promega), 1 × protease inhibitor cocktail (Roche)] for 45 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... RNasin Plus RNase Inhibitor 90 U (Promega), 1 × protease inhibitor cocktail (Roche)] for 45 min ...
-
bioRxiv - Immunology 2024Quote: ... 150 μL of BrightGlo- TritonX solution (90% BrightGlo [Promega, PRE2620] + 1% Triton X [Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were replaced with 90 μL fresh growth medium supplemented with 10 μL MTT reagents (Promega), followed by incubation at 37 °C for 2 h ...
-
bioRxiv - Biochemistry 2024Quote: ... 90 µL of 25 mM ammonium bicarbonate containing 0.5 µg of MS grade Trypsin/LysC (Promega) (enzyme/protein ratio of 1:20 (w/w) ...
-
bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
-
bioRxiv - Molecular Biology 2021Quote: ... All the clones were transfected into the HEK-293T cells at 90% confluency using FuGene 6 as transfection reagent according to the manufacturer’s instructions (Promega). dsiRNAs targeting Rpp30 (10 nM ...
-
bioRxiv - Biophysics 2022Quote: ... DTT was added to a final concentration of 3 mM and the sample was then diluted with 90 μl of 50 mM Tris before adding 0.1 μg of sequencing-grade trypsin (Promega) and incubating at 37 °C overnight ...
-
bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
-
bioRxiv - Biochemistry 2024Quote: ... The translation mixtures were incubated at 30°C for 90 mins followed by the dual-luciferase reporter assay (Promega). The 2μl translation products were added into a white 384-well microplate (PerkinElmer) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were incubated for one hour at 47°C with 2 µg of sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB ...
-
bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
-
bioRxiv - Genetics 2021Quote: Pooled DNA extractions (minimum 90 adult mosquitoes or 3500 L1 larvae) were performed using the Wizard Genomic DNA Purification kit (Promega). A 349 bp locus containing the predicted on-target cleavage site was amplified with primers containing Illumina Nextera Transposase adapters (underlined) ...
-
bioRxiv - Molecular Biology 2020Quote: The non-TOP 5′ UTR sequence of ATP5O (5′-CGGGAGAAG-3′) was identified in published nanoCAGE data 90 and inserted between the T7 promoter and Renilla luciferase in psiCHECK2 (Promega) by Gibson Assembly.
-
bioRxiv - Molecular Biology 2021Quote: ... Then the plasmid constructs (150 ng) were transfected into HEK 293T cells at their 90% confluency using Dual-Luciferase Reporter Assay System (Promega). The Luciferase activity was measured 72 h (48h for DsiRNA and 24 h for plasmid constructs ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
-
bioRxiv - Microbiology 2023Quote: ... The columns were then washed three times with a wash solution (90% methanol; 100mM (final concentration) TEAB) before digestion with 100ug trypsin (Promega) (1/10 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized on 1% agarose gels (90 V, 30-45 minutes) against a 1 kb DNA ladder (Promega). Sanger sequencing was performed by the sequencing unit at the Max Planck Institute for Evolutionary Biology ...
-
bioRxiv - Cell Biology 2024Quote: ... Tris 50mM pH8.0 and samples were heated 5 min at 90°C before digestion in a mix of 500 ng of LysC (Promega) at 37°C for 2h ...
-
A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... Samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
-
bioRxiv - Immunology 2024Quote: ... samples were precipitated using 1:8 vol:vol dilution of each sample in 90% methanol in 100 mM Triethylamonium bicarbonate and digested with trypsin (Promega, #V1115) overnight at 37° C ...
-
bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10 μL of 7.5 M guanidine hydrochloride was added to 10 μL of reaction mixture and incorporated radioactivity was captured on SAM2® Biotin Capture Membranes (Promega, Madison, WI) and quantified using liquid scintillation counting as described before.16
-
bioRxiv - Cancer Biology 2024Quote: ... Dyclonine hydrochloride for 7 days and cell viability was detected by Cell Titer-Glo Luminescent Cell Viability Assay Kit (Promega, catalog number: G7573).
-
bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
-
bioRxiv - Microbiology 2020Quote: Transfection of BKPyV genomic DNA or plasmids containing the agnoprotein into RPTECs or UTA6 cells was performed at 90-95% confluency using ViaFect™ Transfection Reagent (E4982, Promega) at a reagent ...
-
bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
-
bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
-
bioRxiv - Microbiology 2022Quote: ... Plates were further incubated for 90 minutes and fluorescence was measured (λem = 590 nm, λex = 520 nm) using a spectrophotometer (Promega Discover).
-
bioRxiv - Genetics 2022Quote: ... the collected lysates (5% of which were collected as input control) were incubated with 100 μM TASQs (or 100 μM biotin as control) and 90 μg of MagneSphere® beads (Promega) for 2 h at 4°C ...
-
bioRxiv - Biophysics 2024Quote: ... We mixed the solution containing the plasmids and the nicking enzyme was incubated at 37°C for 90 min and immediately purified over the PCR extraction membrane (Wizard® SV, Promega) and specifically skipped the recommended 80°C inactivation step ...
-
bioRxiv - Biochemistry 2024Quote: ... The 5 mL HEK293T cell suspension was combined with the mixture of 10 µL of Nano-Glo® Live Cell Substrate and 90 µL of Nano-Glo® LCS dilution buffer (Promega, Cat.# N2012). The whole mixture of HEK293T with the Nano-Glo® reagents was added to 96 well plates (Greiner Bio-One ...
-
bioRxiv - Microbiology 2021Quote: ... 2) Thermolysin (Promega) or 3 ...
-
bioRxiv - Microbiology 2021Quote: ... (2) Trypsin (Promega), 4 h ...
-
bioRxiv - Cell Biology 2022Quote: ... Erk1/2 (Promega), phospho-Erk1/2 (Promega) ...
-
bioRxiv - Immunology 2024Quote: ... and 2 mg/ml 3-[4,5-dimethylthiazol-2-yl]-5-[3-carboxymethoxyphenyl]2-[4-sulfophenyl]-2H-tetrazolium (PMS, Promega) (1/20 ...
-
bioRxiv - Biophysics 2023Quote: ... and 3 µM CA-JF646 (Promega). Cells were then imaged at room temperature with a 60x objective (N.A ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 2 μL BSA (1 mg/mL) and 2 μL buffer H (Promega) in reaction volume of 20 μL ...
-
bioRxiv - Microbiology 2020Quote: ... at a ratio of 2:2:1 using Fugene®HD (Promega). At 48 h post transfection ...
-
bioRxiv - Cancer Biology 2022Quote: PsiCHECK-2 vector (Promega) was employed to generate luciferase-based reporters for miRNA-target validation ...
-
bioRxiv - Plant Biology 2022Quote: ... 2 µL RNasin (Promega), 250 µL TENT buffer (0.02 M Tris-HCl ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 2 mM MgCl2 (Promega), 0.5 µM of each H and L primer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Trypsin (2 µg; Promega) was added to the samples before incubation at 37°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... phospho-Erk1/2 (Promega), FAK (C-20 ...
-
bioRxiv - Microbiology 2020Quote: ... and RNAsin (Promega, San Luis Obispo, CA) to eliminate contaminating DNA and inhibit RNAse activity ...
-
bioRxiv - Microbiology 2023Quote: ... Human gDNA (Promega, San Luis Obispo, CA) was added to the extracted gDNA from the clinical swabs to reach a total input of 3 μg/130uL for fragmentation and library prep ...
-
bioRxiv - Cancer Biology 2023Quote: ... TAMRA-CA was purchased from Promega (G8251). JQ-1 (HY-13030) ...
-
bioRxiv - Biophysics 2020Quote: ... 2 µL FluoroTect GreenLys (Promega), and 375 ng of plasmid encoding the protein of interest ...