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Citations for Promega :
401 - 450 of 1794 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from one μg RNA with GoScript™ Reverse Transcription System (Promega, Benelux BV) and thereafter diluted to 1:30 (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... In vitro transcription was performed using the T7 RiboMAX Express Large Scale RNA Production System (Promega), followed by Lithium Chloride precipitation to purify the synthesized RNA.
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bioRxiv - Microbiology 2022Quote: In vitro transcription was carried out in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The digoxigenin-labeled antisense cRNA probe was synthesized by in vitro transcription with T7 polymerase (Promega), RNA labeling mix (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Brazil.The GoScript reverse transcription system kit and CytoTox 96 Non-Radioactive Cytotoxicity Assay were from Promega. Brazol reagent was from LGC Biotecnologia ...
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bioRxiv - Microbiology 2022Quote: ... T7 transcription was carried out using T7 RiboMax Express large scale RNA production kit (# P1320, Promega).
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Neuroscience 2023Quote: ... The linearized plasmids were used as templates for in vitro transcription with T3 RNA polymerase (Promega) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: Synthesis of S35-labled CaVβ chimeras was done by coupled in vitro transcription-translation (Promega (L1170)) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega. Amplification of 16S rRNA and fcrX cDNA was made using SsoFast EvaGreen Supermix 2X kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Sly1-HA3 was synthesized with the TNT® SP6 Quick Coupled Transcription/Translation system (#L2080, Promega), according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was reverse-transcribed into cDNA using ImProm-II™ Reverse Transcription System (Promega, WI, USA). Real-time PCR was conducted using the SYBR green PCR reagent (Roche ...
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bioRxiv - Immunology 2023Quote: In-vitro translation assay was performed using TNT® Quick Coupled Transcription/Translation System (Promega #L1170) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: cDNA synthesis was performed with the GoScript™ Reverse Transcription System A5000 (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using oligo(dT) primers and M-MLV Reverse Transcriptase (Promega, Madison, WI) at 42°C for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was transcribed from 300 ng total RNA using the reverse transcription system (Promega, USA) in a final volume of 50 μl ...
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Digoxigenin-labeled probes were then synthesized using in vitro transcription (T7 RNA Polymerase, Promega / Life Technologies), ethanol precipitated ...
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bioRxiv - Microbiology 2024Quote: ... in vitro transcription was carried out using the Ribomax large-scale RNA production systems from Promega, and capping using the ScriptCap m7G capping system (Cellscript).
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Cancer Biology 2021Quote: ... One μg of RNA was reverse transcribed into single stranded cDNA (Go Script Reverse Transcription System, Promega) and subsequently used for qPCR analyses on a Step One Plus real time PCR detection system (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription of the extracted RNA was performed using 1μl of 0.5μg/μl OLIGO dT primers (Promega) and 1μl of RNase free-water and incubating samples at 70°C for 10 minutes and cooling for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed with random hexamer primers (R6) and M-MLV reverse transcriptase (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 800 ng total RNA using GoScriptTM Reverse Transcription Mix using oligo(dT) (Promega). Real-time quantitative PCR was performed by the Taq-Man (rice ...
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bioRxiv - Plant Biology 2020Quote: ... Samples of RNA were reverse transcribed using the GoScript™ reverse transcription system and oligo (dT20) (Promega). RT–qPCR was performed in a 20 μl reaction with the Fast Start Universe SYBR Green Master (ROX ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were in vitro translated using TnT Quick Coupled Transcription/Translation System (Promega, USA, Cat. No. L1170) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was generated from 500 μg purified RNA with the ImProm-II™ Reverse Transcription System (Promega) primed by random primers ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized by reverse transcription using the First-Strand cDNA Synthesis Kit (Promega, Madison, WI). qPCR reactions were performed using Bio-Rad SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: In vitro transcription reactions were carried out using T7 RiboMAX™ Large Scale RNA Production System (Promega) in 10 µl volumes ...
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bioRxiv - Pathology 2021Quote: ... 300 ng of RNA was used to synthesize cDNA using the GoScript™ Reverse Transcription System (Promega). Gene specific primers for Actin (Pn_Actin_qPCR_F and Pn_Actin_qPCR_R) ...
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bioRxiv - Molecular Biology 2020Quote: ... A 5µl aliquot of the sample was added to a 10µl ImProm-II reverse transcription reaction (Promega). The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Developmental Biology 2020Quote: ... 6 uL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... 500 ng of total RNA were reverse transcribed using the GoScript Reverse transcription system kit (Promega, USA), following manufacturer instructions and cDNA samples were then stored at - 20°C until further use.
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Neuroscience 2021Quote: ... ImProm-II™ Reverse Transcription System and GoTaq® DNA polymerase were purchased from Promega (Madison, WI). The RNA Clean & Concentrator™-5 was purchased from Zymo Research (Irvine ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... 5 µL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Evolutionary Biology 2021Quote: Reverse transcription was performed in 20μL final reaction volumes with the GoScript kit (Promega, Madison, WI, USA) and random hexamers (Thermo Fisher ...