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Citations for Promega :
1 - 50 of 2668 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the FLAG-APEX2 was fused in-frame upstream of the GBF1 truncated after HDS1 domain and containing a BFA-resistant Sec7 domain from ARNO (GARG-1060) in a mammalian expression vector pCI (Promega) generating a plasmid pCI-FLAG-APEX2-GARG- 1060 ...
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bioRxiv - Cell Biology 2022Quote: ... MYLK4 (MYLK4-NLuc) and TYK2(JH2 domain) (TYK2(JH2 domain)-NLuc) were kindly provided by Promega. NLuc (nanoluciferase ...
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bioRxiv - Cancer Biology 2019Quote: FAK1 kinase domain (AA393-698) (Promega) (25 μg ...
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bioRxiv - Microbiology 2020Quote: ... 10 μL containing 3 units of DNase I (Promega) and 1 μL of CaCl2 was added ...
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bioRxiv - Molecular Biology 2021Quote: ... PGC-1α full-length and protein domain were translated in vitro in the T7 transcription and translation coupled reticulocyte system (Promega) supplemented with 35S-Methionine ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were resolved in reducing and non-reducing SDS-PAGE gels and membranes were incubated overnight at 4°C with the following antibodies: rabbit polyclonal anti-human p75 intracellular domain (1:1000, Promega); mouse monoclonal anti-HA (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in 50 mM TEAB containing trypsin (Promega) at a 1:20 enzyme-to-protein ratio overnight at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... the protein suspension was digested with 3 μg trypsin (Promega) in 40 μl 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes containing phosphatidylserine and PIP2 (3:1 ratio mol/mol) were purchased (Promega) and prepared to a final concentration of PIP2 at 25 μM ...
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bioRxiv - Cancer Biology 2019Quote: ... cleaved caspase 3 was visualized with antibody (Promega Cat # G7481)) labeled with Qdot 655 Streptavidin conjugate (Thermo Cat# Q10121MP) ...
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bioRxiv - Cell Biology 2020Quote: ... based on a mutated RIIB cAMP-binding domain from PKA (pGloSensor-20F, Promega), which produces rapid and reversible cAMP-dependent activation of luciferase activity in intact cells and is capable of detecting cAMP elevations in the absence of phosphodiesterase inhibitors ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2023Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Neuroscience 2020Quote: ... samples containing 100 μg total protein were mixed with 100 μl Luciferase Substrate (Promega). The kinetics of the luminescence was recorded for 10 min using a TriStar2 S LB 942 Plate Reader (Berthold Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega) and 2 μg LysC (Promega) ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared supernatant was added to a column containing HisLink Protein Purification Resin (V8823, Promega) and his-tag fusion proteins were purified according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... 11S protein was detected with a rabbit polyclonal antibody from Promega, and Tom40 with a rabbit polyclonal antibody produced by Cambridge Research Biochemicals (Billingham ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... The nanoluciferease sequence fused to a PEST domain was amplified from pNL1.2[NlucP] Vector Sequence (Promega) and was cloned into a modified CD813A vector (System Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Plant Biology 2020Quote: ... The supernatant from each sample containing precisely 10 mg of protein was digested with Trypsin Gold (Promega) at 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
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bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Reporter vectors were generated by inserting the Fam210a 3’UTR fragments containing wild-type or mutated seed sequence into the miRNA reporter vector pmirGlo (Promega). All the primers used to generate the reporter vector were listed in supplemental information ...
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bioRxiv - Neuroscience 2021Quote: ... Trpc4 and Trpc5 mRNAs was performed on 3 µl of cDNA from single cells in a 30 µl reaction volume containing 1X GoTaq Flexi buffer (Promega), 2 mM MgCl2 ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 70 μL of a 50/50% mixture containing PBS and the Caspase-Glo 3/7 reagent (Promega). Lysates were incubated at least 2 hours protected from the light at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM CaCl2 at pH 6.4) 0.2 µg/μL protein was mixed with proteases trypsin (Promega sequencing grade), chymotrypsin (BD) ...
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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bioRxiv - Cancer Biology 2021Quote: All kinase assays for AURKA catalytic domain were performed with ADP-Glo™ Kinase Assay Kit (Promega©). For Alisertib the concentrations tested were ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Cell Biology 2022Quote: ... After 3 days Caspase-Glo 3/7 (Promega) and CellTiterGlo (Promega ...