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Citations for Promega :
301 - 350 of 6129 citations for Testosterone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Around 1×105 cells were treated as indicated prior to lysis and RNA isolation using the ReliaPrep™ RNA Miniprep kit (Promega). Approximately 1μg of total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... or 1/6th of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK) and complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.6 or 1 μg extracted RNA was converted into cDNA by reverse transcription using a High Capacity cDNA Reverse Transcription kit (Promega A3500) or a ReverTra Ace qPCR RT Master Mix (Toyobo) ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Cancer Biology 2020Quote: Relative viability was determined in 96 well plates using the CellTiter-Blue Cell Viability Assay (Promega), including a minimum of triplicate wells per sample ...
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bioRxiv - Cancer Biology 2019Quote: ... Remaining plate with cells was used to perform CellTiter-Glo® Luminescent Cell Viability Assay (Promega) to normalize ROS data to number of viable cells ...
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bioRxiv - Immunology 2019Quote: ... the plates were assayed using the CytoTox-ONE Homogenous Membrane Integrity Assay (#G7890, Promega, Madison, WI) as per the kit protocol ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: Three wells of a 6-well plate were lysed were lysed in Reporter lysis buffer (Promega) for 10 min on ice before centrifugation at 17,000 g ...
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bioRxiv - Cancer Biology 2020Quote: ... plates were assayed for cell viability using the CellTiter Blue (CTB) Viability Assay (Promega, Madison, WI) as previously described (47).
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bioRxiv - Bioengineering 2022Quote: ... To each well of the 96-well plate containing supernatant was added CytoTox 96 Reagent (Promega). The plate was shielded from light and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... 96 well plates of putative positive clones were labelled with 500 nM Halo-TMR dye (Promega) for 15 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 24h after transfection luciferase levels determined using Promega dual luciferase assay and GloMax plate reader (Promega).
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bioRxiv - Neuroscience 2020Quote: ... For transfection experiment cells were plated in 24 well plates and transfected with FuGene HD (Promega).
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bioRxiv - Cell Biology 2019Quote: ... Cells were seeded in poly-D-lysine coated plates and transfected with FuGENE® HD (Promega) the following day according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Relative light units (RLU) were measured using a Promega GLOMAX plate reader (Promega, Madison, WI, USA) and values were reported as fold increase over the RLU measured in co-culture of HeLa cells transfected an empty vector (V ...
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bioRxiv - Genetics 2020Quote: ... Dual luciferase activity was measured in 96-well plates using a GloMax Navigator microplate luminometer (Promega). Four biological replicates were used for each experimental condition ...
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bioRxiv - Cancer Biology 2022Quote: ... plate was equilibrated at room temperature for 30 mins and 100 μl CellTitre Glo 3D (Promega) cell viability assay reagent was added to 100 μl of cells in the well with vigorous shaking for 5 mins ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plates were measured once per day for 8 days in a GloMax 96 Microplate Luminometer (Promega). For luminescence ...
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bioRxiv - Microbiology 2022Quote: Adult parasites picked into PBS in 96-well plates were stained with CellTox (Promega, Madison, WI) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells in the 96-well plate were analyzed by Dual-Glo Luciferase Assay Systems (E2920, Promega). Firefly luciferase (F-Luc ...
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bioRxiv - Microbiology 2022Quote: ... Single cycle monolayers were seeded in white bottom plates and read directly for NLuc activity (Promega) on a Synergy HT plate reader (BioTek) ...
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bioRxiv - Developmental Biology 2023Quote: ... Luminescence was measured in raw luminescent units (RLU) using a GloMax® multimodal plate reader (Promega). Reads across a time course were performed using separate wells.
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bioRxiv - Microbiology 2023Quote: ... Luciferase activity was measured using a Glomax plate reader using the Renilla Luciferase Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Absorbances were read using the Dual-Nano-Glo protocol with a GloMax plate reader (Promega #GM3000) in flat-bottom ...
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bioRxiv - Genomics 2023Quote: ... BrdU incorporation was detected by measuring absorbance at 450 nm using a GloMax plate reader (Promega).
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bioRxiv - Physiology 2023Quote: HEK293T cells were cultured in 12-well plates and transfected with polyethylenimine or FuGENE 4K (Promega). LRRC10-Cer and Holo-CaV1.2 venus (Ven)-tagged cDNA pairs (20 ng and 2 μg ...
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bioRxiv - Systems Biology 2024Quote: ... After 48 hours each plate was treated with 25 mL’s of CellTiter Glo (Promega Corp G7572) and read in enhanced luminescence mode on an EnVision Multi-Label plate reader (Revvity Corp.) ...
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bioRxiv - Biochemistry 2023Quote: The initial HaloTag-based APMS (HaloMS) was performed using a commercially available HaloTag ligand plate (Promega, HaloLink 96 Well Plate ...
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bioRxiv - Microbiology 2024Quote: ... The luminescence was measured in a 384 well plate using NanoGlo® live cell buffer (Promega) supplemented with 1:50 of either a membrane impermeable substrate (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed once with PBS and lysed in passive lysis buffer on the plate (Promega). For mitotic cells ...
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bioRxiv - Neuroscience 2024Quote: ... The plate was incubated for 40 min at RT before reading luminescence using GloMax Explorer (Promega) plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... plates were equilibrated to RT for 15 min and CellTiter Glo reagent (Promega, Madison, WI, USA) was added followed by orbital shaking for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... in a 96-well-plate using a blue filter of the GloMax Discover Microplate Reader (Promega). Obtained values were corrected by subtracting background fluorescence value (i.e. ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.
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bioRxiv - Molecular Biology 2019Quote: ... a total of 1□μg of RNA for each sample was reverse-transcribed to cDNA using reverse-transcription kits (Promega, Madison, WI, USA), and real-time quantitative reverse transcription (qRT)-PCR was performed on a 7500 real-time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... plates were used to measure organoid viability using a CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... cell plates were equilibrated to RT for one hour and CellTiter-GloTM Luminescence Cell Viability Reagent (Promega) was added to the cell suspension ...
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bioRxiv - Genomics 2020Quote: ... Luminescence readings for both firefly and renilla were measured via plate reader (Varioskan LUX; Promega DualGlo #E2920). Firefly and renilla values were first blank-subtracted (untransfected cell lysate ...
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bioRxiv - Cancer Biology 2021Quote: ... CellTiter-Blue cell assays were performed in 96-well plates following the manufacturer’s instructions (Promega, Mannheim, Germany) using a FLUOstar Optima microplate fluorescence reader (BMG LABTECH ...