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Citations for Promega :
1 - 50 of 1120 citations for TRNA cytosine 5 Methyltransferase TRDMT1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Transcend Biotin-Lysyl-tRNA (Promega) was incorporated in reactions so that reaction products could be identified using western blotting as above ...
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bioRxiv - Immunology 2021Quote: ... Transcend™ Biotin-Lysyl-tRNA (Promega, Cat.L5061), RNasin® Ribonuclease Inhibitors (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Methyltransferase activity was evaluated using an MTase-Glo Methyltransferase Assay Kit (Promega). The luminescent signal corresponding to SAH production was measured using Centro LB 960 (Berthold Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Methyltransferase assays were performed using MTase-Glo™ Methyltransferase Assay (Promega, USA), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The methyltransferase assays were conducted using the MTase-Glo Methyltransferase Assay (Promega) following the protocol from the manufacturers ...
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bioRxiv - Molecular Biology 2022Quote: Hia5 methyltransferase substrate specificity was measured using the MTase-Glo Methyltransferase Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Microbiology 2023Quote: ... In vitro methyltransferase activity was measured using the MTase-Glo™ methyltransferase assay (Promega, catalog no. V7601) following the protocol of the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: Methyltransferase-Glo™ Assay kit from Promega Corporation (Madison ...
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bioRxiv - Biochemistry 2022Quote: In vitro methyltransferase assays for measuring Nα-methylation were conducted using the MTase-Glo Methyltransferase Assay (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Biochemistry 2021Quote: ... The MTase-Glo™ Methyltransferase Assay Kit (Promega) was used to monitor the methyltransferase activity of SaTrmK ...
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bioRxiv - Cell Biology 2021Quote: ... with Transcend tRNA (Promega, L5061) was utilized to in vitro translate the RhoGEF2 PH domain according to the manufacturer’s recommendation.
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bioRxiv - Biochemistry 2023Quote: ... 5x reaction mixture (Promega MTase-Glo™ Methyltransferase Assay) was added and the mixture was incubated for 30 min at room temperature followed by 30 min incubation with detection reagent (Promega MTase-Glo™ Methyltransferase Assay) ...
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bioRxiv - Cell Biology 2020Quote: ... and Transcend™ tRNA (Promega L5016). Mitochondria extraction was made from synchronized worms at the designated age in mitochondria extraction buffer (5 mM Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2020Quote: ... Native total calf liver tRNA (Promega) and in vitro transcribed tRNASer were aminoacylated using Escherichia coli methionyl tRNA synthetase (for obtaining Met-tRNAiMet ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and the Fluorotect GreenLys tRNA (Promega) labelling system ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with tRNA and RNasin (Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: Native total calf liver tRNA (Promega) and in vitro transcribed tRNAiMet were aminoacylated using recombinant E ...
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bioRxiv - Biochemistry 2023Quote: ... The SAH production was determined with MTase-Glo methyltransferase kit (Promega) using 8 uL of the stopped reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The SAH production was measured using MTase-Glo methyltransferase kit (Promega) using 8 µL of the stopped reaction ...
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bioRxiv - Microbiology 2024Quote: Assays were performed using Promega MTase-Glo Methyltransferase Assay (Promega V7601) with the following modifications ...
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bioRxiv - Synthetic Biology 2023Quote: ... FluoroTectTM GreenLys tRNA was provided by Promega. Glyoxylic acid was purchased from Acros organics ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The non-radioactive MTase-Glo methyltransferase assay kit (Promega, Madison, WI, US) was used for detection of signal on 384-well plates in a total volume of 4 μL ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescent Lys (fLys)-tRNA (Promega, Madison, WI, USA) was also included at 1/10 the reaction volume if required ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated lysine residues were added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend tRNA; Promega) and incorporated into nascent proteins during translation ...
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bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
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bioRxiv - Microbiology 2021Quote: ... The MTase activity was measured using the MTase-Glo Methyltransferase bioluminescence assay (Promega) (27 ...
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bioRxiv - Biochemistry 2022Quote: ... and MTase-Glo™ Methyltransferase Assay Kit was obtained from Promega (Madison, WI). All primers that are described for the sequencing or production of the N-GST METTL7A plasmid were obtained from Integrated DNA Technologies (San Diego ...
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bioRxiv - Biochemistry 2022Quote: ... FluoroTect™ Green Lys-tRNA was purchased from Promega.
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bioRxiv - Genomics 2023Quote: ... Fluorotect Green BODIPY-FL-labeled charged lysine tRNA (Promega) was added to allow protein visualization ...
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bioRxiv - Biochemistry 2021Quote: ... MTase-Glo™ Methyltransferase Assay Kit containing SAM and SAH were purchased from Promega. All other chemicals were purchased from readily available commercial sources and were used without further purification ...
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bioRxiv - Neuroscience 2019Quote: COMT activity was measured using the MTase Glo methyltransferase assay (Promega, Madison, WI, USA) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HMT activity was evaluated using an MTase-Glo Methyltransferases Assay Kit (Promega). The luminescent signal that corresponds to the production of SAH was measured using synergy H1 microplate reader in a white 96-well plate ...
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bioRxiv - Cell Biology 2022Quote: FluoroTect Green lysine-tRNA (green lysine) was purchased from Promega. In-gel imaging of Pkd2-sfGFP or Pkd2 was carried out on a Sapphire biomolecular imager (Azure Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subjected to a pulse with 5 µM biotin-HaloTag ligand (G828A, Promega) diluted in complete medium for 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sequencing grade porcine trypsin and MTase-Glo Methyltransferase Assays were purchased from Promega (Madison, WI). 1,2-Dimyristoyl-sn-glycero-3-PG (DMPG ...
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bioRxiv - Biochemistry 2023Quote: ... The methyltransferase reaction product S-adenosylhomocysteine was quantified using MTase-Glo kit (Promega, Wisconsin, USA) following the manufacturer’s protocol (43) ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of tRNAPhe(GAA) was performed using a 5’ biotinylated complementary oligonucleotide (5’-biotin-TGGTGCCGAAACCCGGGATTGAACCGGGG-3’) coupled to Streptavidin Magnesphere Paramagnetic particles (Promega). Annealing of specific tRNA was performed in 1 x TMA buffer (Tris-HCl pH 7.5 10 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were stopped by addition of TFA to 0.1% and the production of SAH was measured using the MTase-Glo Methyltransferase Assay kit (Promega) according to the manufacturer’s instructions using a Hidex Sense microplate reader..
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bioRxiv - Cell Biology 2023Quote: ... using the rabbit reticulocytes lysates Nuclease-treated kit and the transcendTM tRNA from Promega, as per the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: HaloTag-PEG-biotin ligand (Promega, cat ...
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bioRxiv - Biophysics 2020Quote: ... The quenched reactions were then transferred to a 384-well white round bottom microplate to profile for methyltransferase activity (cumulative mono-, di- and tri-methylation) using the MTase-Glo assay (Promega), which detects the production of S-adenosyl homocysteine (SAH ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Biophysics 2024Quote: ... functionalized with HaloTag Biotin ligand (Promega, G8281), eluted in naïve HEK293T cell lysate with TurboTEV Protease (Accelagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... the tRNA pellet was resuspended in water and incubated with 8 U of RQ1 RNAse-free DNAse (Promega) at 37 °C for 30 min according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega). The samples were incubated for 30min at 22°C prior to magnetic capture of the protein-RNA complex ...
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bioRxiv - Biochemistry 2024Quote: ... In vitro translations were performed with wheat germ extract as directed by the manufacturer (Promega or tRNA Probes) with 3H leucine or 35S methionine ...
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bioRxiv - Developmental Biology 2020Quote: ... HaloTag® PEG-Biotin Ligand (Promega Corporation, G8591) was added to pre-cleared 6His-Halo-MBP PRDM9 to 1 μM final concentration and the mixture was incubated 2 hrs at room temperature ...