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Citations for Promega :
551 - 600 of 708 citations for TMB Substrates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NanoBRET™ Nano-Glo® Substrate - furimazine (#N1663) and HaloTag® NanoBRET™ 618 Ligand (#G9801) were from Promega. Dimethylsulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase intensity was then read on a Luminometer with luciferase substrate according to the manufacturer’s instructions (Promega, PR-E2620). 2 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was transferred into a fresh 384-well assay plate containing NanoGlo Luciferase Assay Substrate (Promega, Madison, WI), and luciferase intensity was measured by EnSpire Plate Reader (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL of cells were mixed with 20 μL of the CellTiter-Glo 2.0 Cell Viability Reagent or Caspase-Glo 3/7 Assay substrate (Promega). Luminescence was measured after 2 min incubation for CellTiter-Glo 2.0 or 30 min incubation for Caspase-Glo 3/7 Assay using the Synergy H1 microplate reader as above ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Neuroscience 2022Quote: ... Half of the virus containing media was aspirated and replaced with luciferase substrate (Bright-glo luciferase assay system, Promega) and incubated for 5 minutes at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and the medium was exchanged to CO2 independent medium (Life Sciences) containing Endurazine substrate (PROMEGA) according to the manufacturers protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of assay cells were mixed with 10 μL of NanoGlo Live Cell Substrate (Promega, Madison, Wisconsin, USA) and 30 μL of LB to make up a total of 50 μL reaction mixture in an opaque ...
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bioRxiv - Bioengineering 2023Quote: ... imaging was performed 10 min after intraperitoneal (i.p.) injection with 200 μL of 15 mg/mL luciferin substrate (Promega). The total flux of luminescence was calculated by gating a region of interest (ROI ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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Contribution of circulating host and microbial tryptophan metabolites towards Ah receptor activationbioRxiv - Biochemistry 2023Quote: ... Luciferase activity was measured using a TD-20e luminometer (Turner Biosystems Inc.) and luciferase substrate (Promega, Madison, WI, USA), per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
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bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... samples corresponding to 4.44×105 cell equivalents of the translation reaction were put on ice and mixed with 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:5000 in 5% milk in TBST were used for detection by the addition of an enhanced chemiluminescence (ECL) substrate (Promega). Western blot quantification was performed using Fiji software (Schindelin et al. ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
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bioRxiv - Developmental Biology 2019Quote: ... The medium was supplemented with 1mM Luciferin (NanoLight Technology, Cat#306A) and 0.5µl of RealTime Glo Cell Viability Assay Substrate (Promega, Cat#G9711). For imaging in pluripotency maintenance conditions ...
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bioRxiv - Plant Biology 2019Quote: ... Samples for luciferase assay were prepared by mixing 25 μL luciferase assay reagent (luciferase assay buffer + 1x final concentration luciferase assay substrate) (Promega) with 5 μL of thawed samples in a 384-well microtiter plate ...
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bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells were then harvested and washed prior to cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with NBT–BCIP (nitro blue tetrazolium–5-bromo-4-chloro-3-indolyl-phosphate) AP substrate (Promega, Catalog # S3771) for in situ cell staining ...
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bioRxiv - Microbiology 2020Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalized to a mock-treated control to generate a fold induction.
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... mice were anesthetized with ketamine-xylazine (100 mg/kg ketamine and 10 mg/kg xylazine) and then retro-orbitally injected with 100 µl of Nano-Glo luciferase substrate (Promega) diluted 1:10 in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were transferred into white 96-well plates and FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and a plate luminometer (Hidex Sense Plate Reader ...
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bioRxiv - Microbiology 2020Quote: ... the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates using the firefly luciferase substrate of the dual-luciferase reporter assay system (Promega) and a Glowmax plate luminometer (Promega).
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bioRxiv - Microbiology 2020Quote: ... the target cells were resuspended at 3×105/mL in prewarmed culture medium that contains EnduRen live cell substrate (Promega) at a final concentration of 17 ng/ml and incubated for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... injecting 50 µl per well of coelenterazine substrate (Nanolight Technologies, 2 µg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction.
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended at 2 x 106 cell mL-1 in complete media and HaloTag substrate conjugated to TMR (Promega) was added at a final concentration of 5 μM ...
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bioRxiv - Cell Biology 2020Quote: Identification of the β5-integrin carboxyl domain as Src substrate was determined using the ADP-Glo Kinase Assay (Promega, V6930) following protocols recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of the lysate was used to measure luciferase activity by sequential mixing with 10 μl of each substrate from the Dual Luciferase Reporter Assay System (Promega). Luminiscence was measured on a Mithras LB 940 microplate reader (Berthold Technologies) ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Biochemistry 2022Quote: ... cells were lysed and assayed with firefly luciferase and renilla luciferase substrates in the Dual-Luciferase Reporter Assay System (E1980, Promega). Firefly luciferase activity (RLU1 ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase reporter gene expression was quantified 48-72 hours after infection upon lysis and addition of Bright-GloTM Luciferase substrate (Promega). Data were retrieved from a BioTek microplate reader with Gen 5 software ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μl of cell lysate or medium were subjected to NanoLuc luciferase assay using Nano-Glo Live Cell Reagent containing 1/50 diluted furimazine substrate (Promega). Relative Luminescence Unit (RLU ...
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bioRxiv - Biophysics 2021Quote: ... The cell suspension was dispensed in a 96-well white bottom plate at a volume of 80 μL per well and loaded with 20 μL of 25 μM Nano-Glo Vivazine Live Cell Substrates (Promega) diluted in the assay buffer ...
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bioRxiv - Microbiology 2022Quote: ... Twenty-five microliters of the substrate diluted at 1/20 in buffer (NanoGlo live assay, Promega, see the manufacturer’s instructions) was added extemporaneously ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium with 1:500 Enduren luciferase substrate (Promega) added ...
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bioRxiv - Microbiology 2022Quote: ... and transferring the contents to a 96-well plate to which the appropriate substrate was added and assayed using a GloMax multi system (Promega).