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Citations for Promega :
251 - 300 of 339 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... treated with 3 units/sample of DNase I for 2 hours (Promega, Madison, WI, USA), and 1-3μl aliquots were taken for reverse transcription (RT ...
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bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
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bioRxiv - Genomics 2019Quote: ... which were subsequently ligated to pGEM-T Vector (pGEM-T Vector System I, Promega, USA), and transformed to DH5α competent cells (Z-Competent E ...
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bioRxiv - Genomics 2021Quote: HDAC activity assays were performed using the HDAC GLO I/II assay kit (Promega, G6430) in 96 well plates following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... smegmatis Complex I (8 μg) was digested with MS-grade trypsin/Lys-C mix (Promega) at a 25:1 (w/w Complex I:protease ratio in 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was isolated with Norgen Biotek kit followed by treatment with DNAse I (Promega) at 37°C for 30 minutes ...
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bioRxiv - Genetics 2024Quote: ... with on-column DNase I digestion using RQ1 RNase-Free DNase (Promega catalog number M6101).
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bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: ... then washed three times in lysis buffer and two times in ammonium biocarbonate (50mM) before peptide digestion (V5280, Promega). Peptides were loaded onto a 3-cm self-packed C18 capillary pre-column (Reprosil 5 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... EV protein samples were alkylated the next day with 50 mM iodoacetamide for 2 hours at ambient temperature in the dark and then digested into peptides with 1.2 µg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol described by Hughes et al (2019) ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control of the instrument setup was monitored using the Promega 6x5 LC-MS/MS Peptide Reference Mix (Promega) and analyzed with the PReMiS software (version 1.0.5.1 ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
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bioRxiv - Biochemistry 2023Quote: ... Rat Gβ1 was fused with a His-tag at the N terminus and with a SmBiT subunit (peptide 86, Promega)34 after a 15-amino acid linker at its C terminus ...
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bioRxiv - Molecular Biology 2023Quote: ... The complexes were washed with RIPA buffer and the samples were treated with DNAse I (Promega) for 30 minutes at 37°C ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated and DNAse I-treated using Maxwell® RSC simplyRNA Tissue kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
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bioRxiv - Biophysics 2020Quote: ... Proteins were digested to peptides by addition of 25 μl of 50 mM ammonium bicarbonate containing 5 μg of trypsin (Promega) and incubating (37°C ...
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bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
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bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... and the supernatant was taken to measure the amount of secreted HiBiT peptide using the Nano-Glo HiBiT Extracellular Detection System (Promega). The resulting luminescence signals were normalized based on the cell viability that was measured using the PrestoBlue Cell Viability Reagent (Thermo).
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bioRxiv - Cell Biology 2022Quote: Methylation of peptides (PSL, Heidelberg, Germany, Table S5) was performed using the bioluminescence-based MTase-GloTM Assay (Promega, Cat#V7601) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... peptides were diluted 4-fold with ABC buffer (Ammonium bicarbonate 50 mM pH8) and digested with Trypsin MS grade (Promega) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed and tryptic peptides were eluted from the beads by overnight digestion in 50 µl trypsin solution (V5111, Promega, 0.01 µg/µl in 100 mM ammonium bicarbonate ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were extracted from 30 eggs and treated with DNase I using ReliaPrep RNA Tissue Miniprep System (Promega). cDNAs were synthesised from the purified RNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Biochemistry 2020Quote: ... For i) Reduced and carbamidomethylated nMPO was digested in technical triplicates using sequencing-grade porcine trypsin (Promega), ii ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB], 0.4 μl RNasin [Promega] ...
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bioRxiv - Developmental Biology 2021Quote: ... DNase I treatment and cDNA synthesis were preformed using the RQ1 RNASE-FREE DNASE 1* (Promega®) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific®) ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were T-cloned using the pGEM®-T Easy Vector System I (cat A1360, Promega) and plated on X-gal/IPTG plates ...
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bioRxiv - Molecular Biology 2023Quote: ... HDAC activity of three technical replicates was measured using the HDAC-Glo I/II Assay (Promega, #G6420) following the manufacturer’s protocol on-bead with an Envision plate reader (Perkin Elmer) ...
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bioRxiv - Immunology 2023Quote: The IL2 URR was cloned into Xho I and Hind III sites of PGL4 Luc vector (Promega) and then ∼500 bp individual sequence representing IL2-IL21 intergenic CNS at −46 ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pGEM-T Easy vector using pGEM-T Easy Vector System I (Promega; CAT A1360). Vectors were transformed into Escherichia coli using the heat-shock method ...
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bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
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bioRxiv - Microbiology 2020Quote: ... The amplified signal peptide fragments were introduced into the plasmid pSUC2 (Prof. Sophien Kamoun, The Sainsbury Laboratory) using T4 DNA ligase (Promega, USA). The yeast invertase assay was used to validate the signal peptides (Jacobs et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Stimulation of P-gP ATPase activity was utilized to indicate the interaction between peptides and P-gP using the P-gP-Glo Assay System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
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bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... DMEM media was aspirated from the cells and 500 µL of hypotonic lysis buffer (50 mM Tris.HCl pH 7.4, 25 units/mL DNase I (Promega), 0.2 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Final enrichment was achieved with a nuclease digestion using DNase I and S1 nucleases (Promega Corporation, Madison, WI). Enriched dsRNA was shipped overnight on dry ice to Quick Biology (Pasadena ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected by using SYBR Green I (GO-Taq PCR Master mix, Promega, Walldorf, Germany; A6002). Suitable reference genes were identified by using geNorm [47] ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected using SYBR Green I (GO-Taq PCR Master mix, Cat: A6002; Promega, Walldorf, Germany). Sequences of the primers used in this study are listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2023Quote: Purified RNA was further subject to a DNase treatment using the RQ DNase I (Promega, Madison, WI, USA) following the manufacturer protocol with small modifications ...