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Citations for Promega :
101 - 150 of 166 citations for Streptavidin AP Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of streptavidin-coupled magnetic beads (Promega, Z5481) per condition were washed three times during 10 min at 4°C (under intermittent shaking ...
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bioRxiv - Physiology 2022Quote: ... Reactions were spotted onto streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE).
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNAs were captured using streptavidin magnetic beads (Promega), washed with salt buffers ...
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bioRxiv - Cell Biology 2020Quote: ... Alkali-phosphatase (AP)-conjugated anti-mouse or anti-rabbit IgG (Promega) as well as AP substrate consisting of 5-bromo-3-chloro-indolyl phosphate (Roche Diagnostics ...
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bioRxiv - Cell Biology 2019Quote: ... As secondary antibody a mouse IgG HRP conjugate was used (Promega, Madison, WI, USA). Detection was carried out by using AceGlow (VWR Peqlab ...
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bioRxiv - Molecular Biology 2022Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega, Fitchburg, United States) was used as secondary antibody ...
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bioRxiv - Biochemistry 2021Quote: 600 μL of Streptavidin-coated paramagnetic particles (Promega, Milan, Italy) were washed three times with 600 μL of PBS-1X (Euroclone ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Biochemistry 2023Quote: ... the efficiency of capture on streptavidin-coated paramagnetic beads (Promega) was evaluated as described before ...
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bioRxiv - Neuroscience 2020Quote: ... Two days exposure to alkaline phosphatase (AP) substrate NBT/BCIP (Promega, S3771) was necessary to detect AP-DIG-labeled Vax1 mRNA at maximal level ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) or anti-mouse IgG-HRP conjugate (1:10,000 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibody labeling was completed using anti-rabbit IgG-HRP conjugate (1:10,000; Promega W401B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... 250 nl of each reaction was spotted on streptavidin-membrane (Promega) using the pintool ...
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bioRxiv - Systems Biology 2022Quote: ... peptides were separated were spotted on streptavidin-conjugated membranes (Promega #V2861) where they tightly associated via their C-terminal biotinylations ...
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bioRxiv - Biochemistry 2020Quote: ... biotinylated RBD-T was immobilized on the Streptavidin MagneSphere particles (Promega) and added to the solution containing phage ...
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bioRxiv - Cell Biology 2022Quote: The AP-1 complexes were expressed in BL21 (DE3) pLysS (Promega, Madison, WI) strains and induced with 0.3 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
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bioRxiv - Microbiology 2019Quote: ... The biotin was used in conjunction with streptavidin coated magnetic beads (Promega) to generate ssDNA from the amplified double-stranded aptamer pool following PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Reacted sequences were isolated with 100 μL Streptavidin MagneSphere paramagnetic beads (Promega) per sample ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h with the secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.) diluted at 1/3000 in 10% Blocking One/TTBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 ng alkaline phosphatase (AP)-conjugated substrates were transfected with FuGENE HD (Promega, E2312). In KRAS related experiments ...
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bioRxiv - Cell Biology 2023Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.5) and libraries were incubated with magnetic streptavidin bead-immobilised bromodomain (Promega) (200 nM ...
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bioRxiv - Biochemistry 2020Quote: ... Reacted sequences were isolated by pull-down with Streptavidin MagneSphere paramagnetic beads (Promega) and eluted with formamide/EDTA ...
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bioRxiv - Genomics 2022Quote: ... After washing with TBST and incubation for 1 hour with anti-Rabbit IgG HRP Conjugate (Promega, 1:2,000) or anti-Mouse IgG HRP Conjugate (Promega ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included goat anti-rabbit IgG HRP-conjugated antibody (1:5,000, #7074S, CST) and goat/rabbit/donkey anti-mouse IgG HRP Conjugate (1:5,000, #W402B, Promega). The membranes for streptavidin blotting were blocked with 3% BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins bound to streptavidin-sepharose matrix were digested with trypsin (Promega, Madison, WI, USA) during 16 h or eluted for western blot analysis ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were spotted onto streptavidin-coated filter sheets (Promega SAM2 biotin capture membrane) and visualized by phosphorimaging on Typhoon FLA 7000 ...
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bioRxiv - Bioengineering 2021Quote: ... The bottom of the chamber was coated with 10 μg ml−1 streptavidin (Promega) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... magnetic beads were prepared by taking 100 μL of Streptavidin Magnesphere Paramagnetic Particles (Promega) and incubated with 5 μM of Spike-Fc-AviTag for 30 minutes ...
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bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed twice with 1X TBS-TT (10X TBS-TT is 10X TBS buffer with 5 mL/L Tween 20 and 5 mL/L Triton X) and once with 1X TBS before incubation with the secondary antibody (IgG anti-rabbit HRP conjugate, Promega) 1:1,000 diluted in blocking solution for 1 hr ...
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bioRxiv - Cell Biology 2021Quote: Biotinylated sAB-K29 (100 µg) was incubated with 1 mL streptavidin-magnetic beads (Promega, Z5482) prewashed three times with PBS buffer in a thermo shaker for 3 h at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The efficiency of biotinylation was subsequently evaluated by capture using streptavidin-coated paramagnetic particles (Promega). The first round of selection was performed using 400 nM of nanodisc-reconstituted MmFLVCR2 diluted in selection buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2019Quote: ... Immuno-signals were visualized with an alkaline-phosphatase (AP)-conjugated anti-rabbit IgG antibody (1:5000, Promega). Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini ...
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bioRxiv - Cell Biology 2020Quote: ... Mcm2-7 was probed with polyclonal UM174 (gift from Bell lab, MIT - (Chen and Bell, 2011)) and anti-rabbit HRP conjugate secondary (Promega W401B), tubulin was probed with monoclonal anti-tubulin antibody (Sigma T5168 ...
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bioRxiv - Biochemistry 2021Quote: ... Reacted sequences were isolated by addition of one sample volume of Streptavidin MagneSphere paramagnetic beads (Promega) per sample ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Molecular Biology 2023Quote: ... each TECprobe-ML reaction was 48 μl due to the omission of 10X (1 μM) streptavidin (Promega) and 10X Start Solution [100 mM MgCl2 ...
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bioRxiv - Biophysics 2022Quote: ... biopanning was performed manually using 200 nM of hSERINC3- MSP1D1 nanodiscs immobilized onto Streptavidin paramagnetic beads (Promega). Following three washes with Selection Buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
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bioRxiv - Bioengineering 2023Quote: HaloTagged AP-2 was visualized in the SUM159 cells through the addition of membrane-permeable JaneliaFluor646-HaloTag ligand (Promega) at a concentration of 125 nM ...
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bioRxiv - Cell Biology 2023Quote: ... chemiluminescent substrate or alkaline phosphatase (AP)-conjugated secondary antibody and Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega). Membranes were imaged using a ChemiDoc MP imaging system (BioRad) ...
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bioRxiv - Plant Biology 2022Quote: ... as primary antibodies and anti-mousse IgG-HRP conjugated secondaries antibodies (Mouse IgG, HRP conjugate W402B, Promega; 1/5000 in TBST, 4 hours). As a loading control we used anti-tubulinα antibodies as primary antibodies (1/1000 in 5 % milk over-night) ...