Labshake search
Citations for Promega :
451 - 500 of 655 citations for St Louis Encephalitis virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Luciferase and β-Gal activity were evaluated in neuronal cell lysates using commercial assay kits (Promega, #E2000, #1500). Luminescence recordings were performed using an Infinite M200 microplate reader with an injector system (Tecan) ...
-
bioRxiv - Physiology 2022Quote: ... Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega) assays according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was determined by mixing 50 μl of protoplast lysate with 50 μl luciferase substrate (Promega, E1501) in a white 96-well plate and light emission was measured at 1 second/well using a microplate luminometer (Centro ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were then processed to measure NAD+ and NADH levels using the NAD/NADH-Glo assay (Promega, G9071) per the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2022Quote: ... Luminescence was measured in cell lysates 24 h after transfection using the Dual Luciferase Reporter Assay Kit (Promega) on a Victor X luminometer (Perkin Elmer ...
-
bioRxiv - Microbiology 2022Quote: ... and then measured luciferase activity in cell lysates by using the Steady-Glo luciferase assay system (Promega Corp.) and a Tecan plate reader ...
-
bioRxiv - Molecular Biology 2023Quote: Firefly and Renilla luciferase activity was measured in cell lysates using the Dual-Luciferase Reporter assay system (Promega) in a GloMax navigator luminometer (Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... and the luciferase relative light unit (RLU) of the lysates was measured using the luciferase assay system (Promega) and Polerstar optima microplate reader (BMG BioLabtech ...
-
bioRxiv - Cancer Biology 2024Quote: ... Luminescence levels yielded by cell lysates were measured using the Luciferase Assay System kit (Promega, Madison, WI, USA) in a Mithras LB 940 plate reader (Berthold Technologies ...
-
bioRxiv - Microbiology 2023Quote: ... Harvested lysates were measured for luciferase units following manufacturer’s instructions of the Luciferase Assay System (Promega, Madison, WI). To determine fold inhibition ...
-
bioRxiv - Cancer Biology 2023Quote: Cell lysates from equal numbers of cells were analyzed for glutathione using GSH/GSSG-Glo assays (Promega, V6611) according to the manufacturer’s protocol.
-
bioRxiv - Biophysics 2023Quote: ... Luciferase activity was recorded by mixing equal volumes of cell lysate and Steady Glo Reagent (Promega, Madison, WI) and reading in a Synergy H1 plate reader (Biotek ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total RNA extraction was performed on HEK-293T cell lysates using the ReliaPrep RNA Cell Miniprep System (Promega) and a genomic DNA digestion was performed using DNase I (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed 24 hours after transfection and the lysates were subjected to dual-luciferase reporter (DLR) assay (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Genetics 2022Quote: ... Firefly and Renilla luciferase activity of cell lysates were sequentially assayed using the Dual-Luciferase Reporter Assay System (Promega) on a GloMax Explorer luminometer (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... RLUs were determined by luciferase assay of cell lysates according to the manufacturer’s protocol (SteadyGlo Luciferase Assay System, Promega) and measured in a Victor Light 1420 plate reader (PerkinElmer ...
-
bioRxiv - Cancer Biology 2019Quote: ... the Luciferase activities of whole cell lysate were measured using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) by a GloMax20/20 Luminometer (Promega) ...
-
bioRxiv - Cell Biology 2019Quote: Target proteins were in vitro transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-Methionine (IsoLabel L-35S Steady Blue ...
-
bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from high titer lysates using the Wizard® Clean-Up Kit (cat. # A7280, Promega, WI, USA). Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
-
bioRxiv - Microbiology 2020Quote: ... Lysates were harvested by washing once with PBS wash followed by addition of 22µl of Passive Lysis Buffer (Promega) under shaking for 15min ...
-
bioRxiv - Molecular Biology 2020Quote: MC Stem-HalV and GUS-(HalV IGR) mRNAs (3 pmol) were translated in 20μl reaction volume of Flexi rabbit reticulocyte lysate (Promega) in the presence of [35S]methionine (>37.0 TBq/mmol ...
-
bioRxiv - Microbiology 2020Quote: ... In vitro transcription/translation was performed with the corresponding primers in Table 1 using pET-pro-IL-1β as a template and following the manufacturer’s recommendations in 10 µl reaction volumes (TNT Coupled Reticulocyte Lysate; Promega). Loading for IL-1R reporter assays was normalized by total IL-1β product measured by enzyme-linked immunosorbent assay (R&D Systems).
-
bioRxiv - Cell Biology 2021Quote: ... The Firefly luciferase and Renilla luciferase levels in the lysates were measured using Dual Luciferase Assay (Promega, Madison, USA). For overexpression by adenoviral mediated delivery ...
-
bioRxiv - Immunology 2022Quote: ... 50 μl of tissue or cell lysates were mixed with 50µl of Bright-Glo™ Luciferase Assay System (Promega) in a 96-well luminometer plate ...
-
bioRxiv - Cell Biology 2022Quote: Total amounts of free cholesterol and cholesterol esters were determined in mouse islet lysates as per the manufacturer’s instructions (Promega). Phosphatidylserine levels were measured in mouse islet lysates as per the manufacturer’s instructions (LSBio).
-
bioRxiv - Microbiology 2021Quote: ... and Tat-dependent increase of luciferase enzyme activity in cell lysates was determined with the Luciferase Assay system (Promega). Luminometric activity was analyzed with a Mithras luminometer.
-
bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μL of cell lysate from each sample to 50 μL of the GSH-Glo™ Reagent 2X (Promega) with DTT (~2.5 mM final ...
-
bioRxiv - Molecular Biology 2019Quote: ... 200 µg lysate was mixed with 0.1 µl RNase A (~3 mg/ml) and increasing concentrations of RNasin (Promega), as indicated ...
-
bioRxiv - Plant Biology 2020Quote: ... We found that using rabbit reticulocyte (TnT® T7 Coupled Reticulocyte Lysate System, Recombinant RNasin® Ribonuclease Inhibitor- Promega) instead of wheat germ extract for in vitro transcription and translation of the ABFs significantly reduces the background noise and increased the quality of peaks that are able to be pulled down from DAP-Seq ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
-
bioRxiv - Microbiology 2020Quote: ... Luciferase activity was determined in 30 μL parasite lysates by adding 30 μL luciferin substrate (Promega Luciferase Assay System) bioluminescence detected with a 10 s integration constant (GloMax®-Multi+ Detection System).4 MB and MMV39004853 were used as positive controls and IC50 determined with non-linear curve fitting (GraphPad Prism 6 ...
-
bioRxiv - Microbiology 2021Quote: ... Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega, Madison WI, USA), and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega ...
-
bioRxiv - Cell Biology 2020Quote: Firefly and Renilla luciferases were assayed from whole cell lysates with the Dual-Luciferase® Reporter Assay System (Promega) as per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and the lysate was used in dual luciferase assays using the Dual-Glo luciferase system (Promega), and measured on the GloMax Discover plate reader (Promega).
-
bioRxiv - Microbiology 2020Quote: ... and lysates used to determine firefly and renilla luciferase activities on a Glomax luminometer using luciferase assay system (Promega) and coeleterazine (Promega) ...
-
bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
-
bioRxiv - Molecular Biology 2022Quote: ... The mRNA at a concentration of 214 ng/µL was translated in the Flexi Rabbit Reticulocyte Lysate System (Promega) for 25 minutes at 32°C resulting in run-off ribosome nascent chain complex (RNC) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysates were diluted 10-fold with 25 mM ammonium bicarbonate and digested with 3 µg of trypsin (Promega, v5111) overnight at 37°C ...
-
bioRxiv - Genomics 2023Quote: ... NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells collected by LCM (29,50) and control genomic DNA (gDNA) (Promega), sequenced using conventional sequencing methods (13).
-
bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
-
bioRxiv - Physiology 2023Quote: ... neutralized with 1M Tris solution and lysates were further processed for measurement using the Glucose-Glo Assay (Promega, GmbH) according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2023Quote: ... 2 µL of yeast lysate was used as a template in a 10 µL GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...