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Citations for Promega :
101 - 150 of 1502 citations for Somatostatin Receptor 4 SSTR4 Rabbit Polyclonal affinity purified biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... microdialysed and 32P-labeled using Prime-a-Gene® Labeling System (Promega). Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: The HaloTag-holocomplexes were labeled with HaloTag Alexa Fluor 488 Ligand (Promega) at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and antisense DIG-labeled RNA was synthesized using SP6 RNA polymerase (Promega).
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... Biotin-containing handles were made with a PCR on pBluescript SK+ (stratagene) with GoTaqG2 (Promega, M7845), in the presence of 1/5 biotin-16-dUTP (JenaBioscience ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Genomics 2021Quote: ... polyclonal anti-mouse IgG-HRP raised in goat (W402B, 1:3,000; Promega) and polyclonal anti-rabbit IgG-HRP raised in goat (R1364HRP ...
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bioRxiv - Biochemistry 2021Quote: ... Beads from each affinity purification were then resuspended in 200 μl 50 mM TEAB pH8.5 containing 0.01% ProteaseMAX (Promega), 10 mM TCEP ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Molecular Biology 2020Quote: ... HaloTag-positive cells were labeled with Janelia Fluor 646 HaloTag fluorescent Ligand (Promega). GCaMP3-KI cells exhibited strong fluorescence when subjected to a flow cytometer maybe due to shear or/and mechanical stress (Fig ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... BICD2N or HaloTag alone were labeled with TMR-HaloTag ligand (Promega, Madison, WI) 18–24 h post-transfection then lysed in 100 μl lysis buffer [40 mM Hepes (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega). In situ hybridization was performed a combination of what has been described previously (50 ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Cell Biology 2023Quote: ... BrdU/BrdC-labeled DNA was digested with 10 U/μl Exonuclease III (Promega) in buffer supplied by the manufacturer for 30 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... we labeled the cells with 2.5 µM HaloTag-Ligand (HTL) TMR (Promega #G8251) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
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bioRxiv - Microbiology 2022Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of nsp4 RNA using mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of N RNA using mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... or purified LgBiT-substrate cocktail (Promega). After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... or purified LgBiT-substrate cocktail (Promega), the blots were imaged and processed with a FluorChem E (Protein Simple).
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4% PEG-8000 (Promega), 2% FBS (Biosera) ...
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bioRxiv - Genomics 2023Quote: ... 4 mM DTT (Promega), and 1x CutSmart buffer (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were labeled with 100 nM Janelia Fluor 646 HaloTag ligand (Promega, GA1120) for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... A digoxigenin (DIG)-labeled antisense (AS) probe was synthesized using the T7 polymerase (PROMEGA), for hybridization on pre-parasitic stage 2 juvenile nematodes (ppJ2) ...
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bioRxiv - Biochemistry 2022Quote: ... A portion of cells were labeled with 100 nM HaloTag TMR ligand (Promega; G8252) and fluorescent colonies were identified using a TIRF microscope ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
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bioRxiv - Neuroscience 2019Quote: ... and DNaseI-purified (Promega, Madison, WI, USA). cDNA was generated using the Superscript II protocol (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR DNAs were gel purified (Promega Wizard SV Gel and PCR Clean-up System #A9282 ...
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bioRxiv - Genetics 2023Quote: ... Purified RNA was treated with DNase (Promega) at 37°C for 30 min ...
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bioRxiv - Genomics 2023Quote: ... purified Homo sapiens DNA (Promega, Madison, WI) with a microbial community standard (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... A total of 400ng of DNA was labeled with Cy3 while the reference DNA (Promega) was labelled with Cy5 ...
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bioRxiv - Biochemistry 2019Quote: Prior to the single-molecule motility assays dynein was labeled with Halo488 dye (Promega, G1001) as previously described (Bhabha et al ...
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bioRxiv - Microbiology 2021Quote: ... Probes were then labeled with α-32P dCTP using the Prime-a-gene kit (Promega). For high molecular weight northern blot analysis to detect DENV2 genomic RNA ...
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bioRxiv - Genomics 2019Quote: ... RNA probes were labeled by in vitro transcription of plasmids with T7 RNA polymerase (Promega) in the presence of Biotin RNA labeling mix (Roche) ...