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Citations for Promega :
201 - 250 of 2840 citations for Somatostatin Receptor 1 SSTR1 Mouse Monoclonal biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... SCC1-Halo was purified and fluorescently labeled with Janelia Fluor 549 HaloTag (Promega) as previously described (5).
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bioRxiv - Molecular Biology 2020Quote: ... HaloTag-positive cells were labeled with Janelia Fluor 646 HaloTag fluorescent Ligand (Promega). GCaMP3-KI cells exhibited strong fluorescence when subjected to a flow cytometer maybe due to shear or/and mechanical stress (Fig ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... BICD2N or HaloTag alone were labeled with TMR-HaloTag ligand (Promega, Madison, WI) 18–24 h post-transfection then lysed in 100 μl lysis buffer [40 mM Hepes (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega). In situ hybridization was performed a combination of what has been described previously (50 ...
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bioRxiv - Biophysics 2020Quote: eGFP was purified and labeled with HaloTag Succinimidyl Ester (O4) Ligand (Promega, P6751) as previously described (Huang ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Cell Biology 2023Quote: ... BrdU/BrdC-labeled DNA was digested with 10 U/μl Exonuclease III (Promega) in buffer supplied by the manufacturer for 30 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... we labeled the cells with 2.5 µM HaloTag-Ligand (HTL) TMR (Promega #G8251) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Plant Biology 2023Quote: ... The proteins were separated by 12% Tris–Glycine– SDS–PAGE and the gel was electroblotted to a PVDF membrane (0.2 µm) followed by immunodetection using Anti-HaloTag® Monoclonal Antibody (1:5000) (Promega, Madison, Wisconsin, United States) in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Biophysics 2023Quote: ... The 12-230 kDa separation module was used for PCNA (CST, D3H8P, Rabbit, 1:100 dilution, #13110), b-Actin (CST, D6A8, Rabbit, 1:50 dilution, #8457S) and Halo (Promega, Mouse, 1:10 dilution, #G9211) detection ...
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bioRxiv - Plant Biology 2020Quote: ... an immunoblot with anti-halo antibody (Anti-HaloTag® Monoclonal Antibody-Promega) was performed to confirm protein expression and binding efficiency for each TF ...
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bioRxiv - Plant Biology 2020Quote: The antisera (and their sources) were as follows: monoclonal anti-HA (Promega), anti-phospho-LHCB2 (Agrisera ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a 1:20,000 dilution of anti-mouse IgG HRP conjugated secondary antibody (Promega) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Neurite outgrowth was visualized by immunostaining with mouse anti-β III tubulin (1:1000; Promega) followed by incubation with Alexa 594-conjugated anti-mouse secondary antibodies (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... or secondary antibodies: anti-mouse IgG horseradish peroxidase (HRP) (Promega, Madison, WI, W402B, 1:10,000), anti-rabbit IgG HRP (Promega ...
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bioRxiv - Immunology 2023Quote: ... followed by goat anti-mouse immunoglobulin conjugated to alkaline phosphatase (Promega S3721, dilution 1:7500). Focuses of infection were revealed using NBT/BCIP reagent (Promega) ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-T-Cofilin 1:2000 (Bamburg lab, 1439 or Cell Signalling, 5175) and mouse anti-β3-tubulin 1:2000 (Promega, G7121) diluted in 1%BSA/PBS and incubated overnight at 4ºC ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were labeled with 100 nM Janelia Fluor 646 HaloTag ligand (Promega, GA1120) for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... A digoxigenin (DIG)-labeled antisense (AS) probe was synthesized using the T7 polymerase (PROMEGA), for hybridization on pre-parasitic stage 2 juvenile nematodes (ppJ2) ...
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bioRxiv - Biochemistry 2022Quote: ... A portion of cells were labeled with 100 nM HaloTag TMR ligand (Promega; G8252) and fluorescent colonies were identified using a TIRF microscope ...
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bioRxiv - Immunology 2023Quote: Activation of chemokine receptors was detected by measuring ß-galactosidase activity using the commercially available Beta-Glo assay system (Promega Corp, Madison, WI, USA) and luminescence signal was recorded on a multimodal plate reader (Enspire 2300 ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The genome-edited monoclonal cell populations were identified by PCR (GoTaq Polymerase, Promega) and then verified by western blot analysis and imaging.
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 400ng of DNA was labeled with Cy3 while the reference DNA (Promega) was labelled with Cy5 ...
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bioRxiv - Biochemistry 2019Quote: Prior to the single-molecule motility assays dynein was labeled with Halo488 dye (Promega, G1001) as previously described (Bhabha et al ...
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bioRxiv - Microbiology 2021Quote: ... Probes were then labeled with α-32P dCTP using the Prime-a-gene kit (Promega). For high molecular weight northern blot analysis to detect DENV2 genomic RNA ...
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bioRxiv - Genomics 2019Quote: ... RNA probes were labeled by in vitro transcription of plasmids with T7 RNA polymerase (Promega) in the presence of Biotin RNA labeling mix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... stimulated cells were labeled with HaloTag® Ligands for Super Resolution Microscopy-Janelia 549 (Promega) accordingly to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... cells were labeled with the relevant Halo-dye (Promega, Janelia Fluor® HaloTag® Ligands) or SNAP-dye (SNAP-Cell® 647-SiR ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Molecular Biology 2019Quote: ... Purification of tRNAPhe(GAA) was performed using a 5’ biotinylated complementary oligonucleotide (5’-biotin-TGGTGCCGAAACCCGGGATTGAACCGGGG-3’) coupled to Streptavidin Magnesphere Paramagnetic particles (Promega). Annealing of specific tRNA was performed in 1 x TMA buffer (Tris-HCl pH 7.5 10 mM ...
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bioRxiv - Biophysics 2023Quote: 5 μM purified EGFP-YbbR-Lphn3 GAIN-HaloTag protein in HBS was mixed with 5.2 μM HaloTag PEG-Biotin Ligand (Promega, G8592) and incubated at room temperature for 2 hours to biotinylate the CTF of the GAIN construct ...