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Citations for Promega :
1 - 50 of 2980 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... or transfection carrier DNA (Promega E4881) as a negative control.
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bioRxiv - Systems Biology 2022Quote: ... 9 μg Transfection Carrier DNA (Promega) and 30 μL FuGENE HD Transfection Reagent (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted in carrier DNA (E4881, Promega) to obtain a final DNA amount of 50 ng/well ...
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bioRxiv - Microbiology 2020Quote: ... duplicate wells were transfected with 1 µg HDM_Spike_RBD_B7-1 plasmids and 1 µg of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Cancer Biology 2022Quote: The CLK family of nanoBRET assays were run as directed by Promega. Briefly ...
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bioRxiv - Microbiology 2020Quote: ... and 0.5 μg of carrier DNA (Promega, E4881) to achieve an 8.5:1 ratio of APN:TMPRSS2 ...
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bioRxiv - Cell Biology 2023Quote: ... Carrier DNA was encoded in pFN5K vectors (Promega). For cellular BRET target engagement experiments ...
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bioRxiv - Cell Biology 2021Quote: ... and 750ng of transfection carrier DNA (Promega, pGEM2 plasmid). Cells were expanded in 10cm plate on day 2 and analyzed by fluorescent flow cytometry on day 3 with an Amnis Imagestream X (Luminex ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A2-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... consisting of 9 µg/mL of carrier DNA (Promega) and 1 µg/mL of CSNK2A-NLuc fusion DNA in Opti-MEM without serum (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Biochemistry 2022Quote: ... fusion constructs were diluted together into Transfection Carrier DNA (Promega) at a mass ratio of 1:9 (mass/mass/mass ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... was prepared with 9 μg/mL of Carrier DNA (Promega) and 1 μg/mL of NL-BRSK2 to achieve a total volume of 1.05 mL ...
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bioRxiv - Microbiology 2020Quote: ... as carrier DNA and a luciferase-encoding plasmid (pGL4.26, Promega). qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Nluc/Abl fusion constructs were mixed with carrier DNA (pGEM3ZF-, Promega) at a mass ratio of 1:10 (mass/ mass ...
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bioRxiv - Cell Biology 2023Quote: ... NLuc/target fusion constructs were diluted into Transfection Carrier DNA (Promega) at a mass ratio of 1:9 (mass/mass) ...
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bioRxiv - Cancer Biology 2022Quote: ... to 10.5mL of 2.0 x 10^5 of Hek293 Cells (ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525uL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nluc/target fusion constructs were diluted into Opti-MEM followed by Transfection Carrier DNA (Promega) at a mass ratio of 1:10 (mass/mass) ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were treated with either DMSO (carrier solvent; 0.1% final) or HaloTag NanoBRET 618 Ligand (Promega) and 40 µL/well were plated in a white 384-well plate (Corning #3570) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) with 2-5µg plasmids pre-digested by XcmI and 50 µg of herring sperm carrier DNA (Promega), then 700 µL of yeast transformation buffer (40% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... qPCR was then performed to detect viral gene ORF26 and luciferase from the carrier plasmid using GoTaq polymerase (Promega) and the ΔΔCT method ...
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bioRxiv - Synthetic Biology 2022Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL) (herring sperm DNA, Promega) were added onto the cell pellet ...
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bioRxiv - Synthetic Biology 2023Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL, herring sperm DNA, Promega), was added onto the cell pellet ...
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bioRxiv - Microbiology 2020Quote: ... 12 U RNasin (Promega) and 0.9% NP-40 (Thermo Scientific Pierce ...
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bioRxiv - Genetics 2024Quote: Conditioned media from NL-WNT2B-expressing cells were collected after 48h transfection with plasmids encoding HT7-tagged carriers or entire GPC ectodomains and were captured on HaloLink beads (Promega) as previously described(65) ...
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bioRxiv - Cancer Biology 2024Quote: ... expressing CD155 wildtype 5′ UTR or various mutant constructs (200ng) and a carrier pUC19 plasmid (400ng) per well using Fugene HD (Promega, E2311) at a 3:1 Fugene:DNA ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Cell Biology 2020Quote: ... 12 μl FuGene HD (Promega) and a total of 3 μg from all plasmids ...
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bioRxiv - Cancer Biology 2020Quote: ... 12 and pRL-TK (Promega) in a ratio of 100:1 using ViaFect transfection reagent (Promega Cat# E4982) ...
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bioRxiv - Cancer Biology 2021Quote: ... the NL-CLK1 or CLK2-NL or CLK4-NL fusion construct was diluted with carrier DNA - pGEM- 3Zf (-) (Promega, Madison, WI, USA) at a mass ratio of 1:10 (mass/mass) ...
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bioRxiv - Molecular Biology 2022Quote: ... using 12 uL of Viafect (Promega) in 200 uL OptiMEM (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Phorbol 12-myristate 13-acetate (Promega) and ionomycin (Tocris ...
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bioRxiv - Microbiology 2023Quote: ... 12 μl Viafect Transfection Reagent (Promega) and MEM without antibiotics and FCS to a final volume of 300 μl ...
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Synthetic Biology 2022Quote: ... three 12-μl aliquots of RQ1DNase (Promega) were added to reduce the viscosity of the lysates and samples were rotated for 15 min at 4 °C after each dose of the enzyme ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Molecular Biology 2023Quote: ... with 12 µg Trypsin (sequencing-grade modified, Promega). After 16h incubation at 37°C insoluble proteins were pelleted by centrifugation (21,000 x g ...
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bioRxiv - Immunology 2024Quote: ... and 0.5 µg Oligo(dT)12-18 (Promega) and incubated for 5 min at 70°C ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...