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Citations for Promega :
51 - 100 of 430 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Oligo(dT)15 primers (Promega, #c1101) and ImProm-II Reverse Transcriptase (Promega ...
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bioRxiv - Microbiology 2024Quote: ... and oligo(dT)15 primer (Promega). The mRNA transcript levels of selected IVSPER genes were measured by quantitative reverse transcription-PCR (qRT-PCR ...
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bioRxiv - Systems Biology 2023Quote: HEK293 cells stably expressing μOR or μOR-APEX2 were transiently transfected with the cAMP biosensor pGLO-20F (Promega). Prior to agonist stimulation ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Biochemistry 2021Quote: Cells were transfected for 48 h with 15 µg DNA per 15 cm dish using Fugene HD (Promega) following the manufacturer’s instructions using plasmids encoding full-length FLAG-tagged MeiP26 protein ...
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bioRxiv - Bioengineering 2019Quote: ... cells were transfected using FuGene HD transfection reagent according to their standard protocol for HEK293 cells (Promega, Madison, WI). DNA plasmids were cloned in Dam-negative E ...
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bioRxiv - Immunology 2020Quote: ... The sleeping beauty transposon system was stably transfected into HEK293 EBNA cells using FuGENE® HD (Promega GmbH, USA) in DMEM/F12 (Merck ...
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bioRxiv - Biochemistry 2023Quote: The recruitment of PTH1R to β-arrestin was detected in HEK293 cells using the NanoLuc Binary System (NanoBiT; Promega). The Lgbit subunit was fused to the C-terminus of PTH1R and the SmBiT subunit was fused to the N-terminus of β-arrestin ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
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bioRxiv - Biochemistry 2023Quote: BromoTag cell lines were generated in HEK293 cells via simultaneous transfection of two vectors at a 4:1 reagent:DNA ratio with FuGENE 6 (Promega). The first vector was a pMK-RQ vector containing 500 bp homology arms on either side of either an eGFP-IRES-BromoTag or eGFP-IRES-HiBiT-BromoTag sequence for integration into MCM4 and BRD4 ...
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bioRxiv - Neuroscience 2021Quote: ... and oligo(dT)15 primer (Promega, C1101).
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bioRxiv - Bioengineering 2019Quote: ... 15 μL 2x GoTaq Master Mix (Promega), 1.5 μL 10 μM FP ...
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bioRxiv - Cell Biology 2021Quote: ... and an Oligo(DT)15 primer (Promega). RT-qPCR was performed with Sybr Green® Lightcycler Green I Master mix (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... using Oligo(dT)15 primers (Promega #C110A). The following primers were used to amplify the full length of LIMK1 cDNA by PCR ...
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bioRxiv - Biochemistry 2020Quote: ... 15 μl Dye solution (Promega cat. #G4102) was added into each well ...
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bioRxiv - Molecular Biology 2019Quote: ... 250ng oligo (dT)15 primer (Promega, #C1101), 1× First-Strand Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The pRL-TK plasmid (Promega; 15 ng) expressing Renilla luciferase was included as an internal control ...
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bioRxiv - Cell Biology 2020Quote: ... and oligo (dT) 15 Primer (Promega: C1101). qPCRs were performed in triplicate using primers against ZNF598 ...
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bioRxiv - Genomics 2021Quote: ... and Oligo(dT)15 primers (C110A, Promega), following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... with Oligo(DT)15 Primer (Promega C1101). Q-RT-PCR reactions used Lightcycler 480 SYBR Green I Master Mix (Roche 04707516001 ...
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bioRxiv - Microbiology 2022Quote: ... using an oligo(dT)15 primer (Promega). Quantitative PCR (primers Tb927.4.1270-F-01 and Tb927.4.1270-R-04 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Oligo(dt) 15 primers (Promega, C1101) containing RNasin® Plus RNase inhibitor (Promega ...
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bioRxiv - Immunology 2023Quote: ... and an Oligo(dT)15 primer (Promega). qPCR was performed with the Fast EvaGreen qPCR Master Mix (Biotium ...
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bioRxiv - Microbiology 2024Quote: ... 15 µL of lysis solution (Promega G182A) was added 1 h prior to XTT working solution ...
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bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2019Quote: Approximately 5 × 107 control cells and 2 × 107 sort3 PIGS-KO HEK293 cells were used for genomic DNA extraction by Wizard Genomic DNA Purification Kit (Promega). Approximately 325 µg of genomic DNA from control cells and 30 µg of genomic DNA from sort3 cells were used for amplification of gRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Immunology 2020Quote: ... Recombinant DJ-1 protein (1 μM) was added to the transfected HEK293 cell lines and luciferase activities were measured using a Luciferase Assay System (Promega). 10 μg mL−1 of peptidoglycan (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Immunology 2022Quote: ... 5×106 HEK293 cells were plated in 15cm petri dishes and transfected the day after with 50 μL FuGENE (E2311, Promega), 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 500 ng Oligo (DT)15 (Cat. # C1101, Promega) and 100 ng random hexamer (Cat # C1181 ...
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bioRxiv - Microbiology 2020Quote: ... RT was primed with oligo dT(15) (Promega) and held for 1 h at 42 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and Oligo(dT)15 primers (Promega, United States) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2019Quote: ... with 2 µM Oligo(dT)15 primers (Promega) and 10 U RNase OUT in a 20 µl reaction at 37ºC for 90 min ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of Kinase detection reagent (Promega kit) was added to the wells ...
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bioRxiv - Immunology 2023Quote: ... dNTP Mix and Oligo (dT)15 Primer (Promega), Low Input Quick Amp Labeling Kit ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Neuroscience 2021Quote: Luminescence assays to monitor cAMP levels were performed as we described previously (Siuda et al., 2015). Briefly, HEK293 cells (ATCC, cat. # CRL-1573) were co-transfected with PPO-Venus and pGloSensor-22F (Promega E2301) plasmids using JetPrime reagent (Polyplus ...
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bioRxiv - Molecular Biology 2023Quote: ... NLS-NSD2-mVenus WT and T1150A plasmids were transfected into 70 % confluent HEK293 SETD2 knockout cells using FuGENE® HD Transfection Reagent (Promega). NLS-mVenus empty vector was used as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of the 3 Cas9-sgRNA plasmids (150ng/μl) was transfected into HEK293 cells at 70% confluency in fresh medium using FuGENE® HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC-aCM or HEK293 cells were incubated with a labeling solution containing both cell-impermeant HTL-Alexa488 (Promega G1001, 1 µM) and Cellmask Deep Red Plasma Membrane stain (Invitrogen C10046 ...
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bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...