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Citations for Promega :
351 - 400 of 486 citations for Serum Free RocketFuel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... The plasmid was purified using the PureYield™ endotoxin-free Plasmid Maxiprep System (Promega, Lyon, France) and then verified by restriction enzyme digestion and by sequencing (Eurofins MWF Operon ...
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bioRxiv - Microbiology 2021Quote: ... any amount of DNA was removed using DNase (RQ1 RNase-Free DNase, Promega, Madison, Wisconsin, USA) and measured using Qubit (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: RNA isolated from cells (1 μg) was digested using the RQ1 RNase-Free Dnase (M6101, Promega) and reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNAs were treated with 1 unit of RQ1 RNase-Free DNase (Promega) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Genomic DNA was then digested out using the RQ1 RNase-Free DNase (Cat. No. M6101; Promega). cDNAs were obtained from 400 ng of RQ1-treated RNA using 1 μl of GoScript Reverse Transcriptase (Cat ...
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bioRxiv - Plant Biology 2023Quote: ... 2μg of RNA per sample were treated with DNase RQ1 RNase-Free (Promega, Madison, WI, USA); and used for synthesis of cDNA with Super-Script IV enzyme (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a second DNase treatment was performed with RQ1 RNase-free DNase (Promega, Charbonnières-les-Bains, France), followed by RNA clean-up using the RNeasy Plant Mini Kit ...
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bioRxiv - Genomics 2023Quote: ... Samples were then allowed to cool for 5 minutes and 5μl of RNase (DNase-free; Promega) was added and incubated at 37ºC for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: Carried over DNA was removed by off-column treatment with RQ1 RNase-free DNase (Promega cat# M610140). qPCR was done using an Applied Biosystems 7500 Fast Instrument with Quantitation -Standard Curve experimental type and Takyon Low ROX SYBR 2X MasterMix blue dTTP (Eurogentec cat# UF-LSMT-B0710 ...
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bioRxiv - Genetics 2020Quote: ... The microinjection reagents were combined in nuclease-free T10E0.1 pH 7.5 (Integrated DNA Technologies; Tris 10mM, EDTA 0.1mM) supplemented with Rnasin (Promega) at 0.2 U/ul ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μl of template and ultrapure DNAse/RNAse free distilled water (Promega Corporation, Fitchburg, WI, USA).
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Pathology 2020Quote: ... and contaminating DNA was removed by using the RNase-free RQ1 DNase (Promega Corp., Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of total RNA from each sample was treated with RQ1 RNase-Free DNase (Promega Co.) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of RNA were depleted from contaminating DNA by treatment with RQ1 RNase-free DNase (Promega) and were reverse-transcribed with Superscript IV Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Any contaminant genomic DNA was degraded by DNase treatment (RQ1, RNase-free DNase, Promega Corp., WI, USA). cDNA was generated using M-MLV reverse transcriptase (Promega Corp. ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from 2µg of DNA free RNA using MMLV reverse transcriptase and random primers (Promega). Quantification of the cDNA was carried out in the Rotor gene 3000A machine (Corbett ...
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bioRxiv - Developmental Biology 2021Quote: ... DNase I treatment and cDNA synthesis were preformed using the RQ1 RNASE-FREE DNASE 1* (Promega®) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific®) ...
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bioRxiv - Microbiology 2022Quote: ... Residual genomic DNA was removed by treating with 10 units/sample of RNAse-free RQ1 DNAse (Promega) for 1h at 37°C followed by phenol-chloroform extraction and ethanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was removed from the total RNA by treatment with RQ1 RNase-free DNase (Promega, M6101). cDNA synthesis was carried out with the RevertAid First Strand cDNA synthesis Kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of DNAse free RNA was used for cDNA synthesis using ImProm-II Reverse transcription system (Promega), as per the manufacture’s recommendation ...
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bioRxiv - Synthetic Biology 2022Quote: ... the tRNA pellet was resuspended in water and incubated with 8 U of RQ1 RNAse-free DNAse (Promega) at 37 °C for 30 min according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Any residual genomic DNA was removed by treating RNA with RQ1 Rnase-free DNase (Promega, Madison, WI, USA) at 37°C for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... Free UDP was measured as a proxy for reaction progress using the UDP-Glo enzyme assay kit (Promega) (Zegzouti et al. ...
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bioRxiv - Cell Biology 2021Quote: ... 800 ng of total RNA were freed from genomic DNA using the RQ1 RNase-Free DNase Kit (Promega). Reverse transcription was performed using 145,4ng of the total DNase-treated RNA using the TaKaRa PrimeScript RT Master Mix Kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Microbiology 2022Quote: ... Additional DNAse treatment was also performed on the harvested RNA using RQ1 RNase-Free DNase (Promega, catalog # M6101) according to the manufacturer’s protocol to remove any carryover of double-stranded or single-stranded DNA ...
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bioRxiv - Plant Biology 2022Quote: ... and the concentration was measured before and after DNase I digestion (RQ1 RNase free DNase I, Promega®) using spectrophotometry (OD260) ...
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bioRxiv - Cancer Biology 2022Quote: ... 200 μL of cell-free supernatants were mixed with 50μL CellTiter-Glo Luminescent Cell Viability Assay (Promega, G7571) in white 96-well plates (Greiner ...
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bioRxiv - Bioengineering 2023Quote: ... RNA samples prepared were treated with DNase according to the manufacturer’s instructions (RQ1 RNase-Free DNase from Promega), quantified with a Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed in antibiotic free medium using FuGENE® HD transfection reagent (Promega Corporation, Durham, NC, USA) following the manufacturer’s instructions with a final plas-mid amount of ∼100-200 ng per well ...
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bioRxiv - Biochemistry 2022Quote: ... eluted in 12 µl of nuclease free water and quantify using the dsDNA QuantiFluor Dye System (Promega, #E2670).
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted using the Maxwell® RSC simplyRNA Cells and RNase-free DNase Kit (Promega; AS1390) using an automated RNA/DNA isolation instrument (Maxwell ...
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bioRxiv - Molecular Biology 2020Quote: Cells were blocked by pipetting 50ul of 5% Bovine serum albumin (BSA) (Promega, Cat: W3841) directly onto slides ...
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bioRxiv - Genomics 2021Quote: The cffDNA was extracted from serum using a Maxwell RSC cffDNA Plasma Kit (Promega; AS1480) by the Primate Assay Laboratory Core at the California National Primate Research Center ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was prepared using TRI reagent (MRC) according to the manufacturer’s instructions and treated with RQ1 RNAse-free DNase (Promega) and proteinase K (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... and DNA was digested using RQ1 RNase-Free DNase kit according to manufacturer’s instructions (Promega, Madison, WI, Cat#: M6101). Finally ...
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bioRxiv - Cell Biology 2022Quote: Total amounts of free cholesterol and cholesterol esters were determined in mouse islet lysates as per the manufacturer’s instructions (Promega). Phosphatidylserine levels were measured in mouse islet lysates as per the manufacturer’s instructions (LSBio).
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bioRxiv - Molecular Biology 2022Quote: ... Chromatin-bound proteins were solubilised by incubation with 1320 U of DNase I (RQ1 RNase-free DNase I, Promega) on ice for 20 minutes ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 mini tablet of Complete EDTA-free protease inhibitor and 2 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega). RNA was extracted using the RNASpin Mini kit (GE Healthcare ...