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Citations for Promega :
51 - 100 of 886 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... After incubation with Goat anti-Rabbit and Goat anti-Mouse HRP-conjugated secondary antibodies (Promega), proteins were visualized using chemiluminescent peroxidase substrat detection reagents (Sigma) ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... microdialysed and 32P-labeled using Prime-a-Gene® Labeling System (Promega). Filters were prehybridized for 2 hr at 65°C in Rapid-hyb solution (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: The HaloTag-holocomplexes were labeled with HaloTag Alexa Fluor 488 Ligand (Promega) at room temperature for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and antisense DIG-labeled RNA was synthesized using SP6 RNA polymerase (Promega).
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... washed and incubated with secondary antibody goat-anti-rabbit-HRP or goat-anti- mouse-HRP (Promega) for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Goat anti rabbit HRP (Promega (W4011)) 1/5000 ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP (Promega) was used as secondary antibody ...
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bioRxiv - Microbiology 2019Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Beads from each affinity purification were then resuspended in 200 μl 50 mM TEAB pH8.5 containing 0.01% ProteaseMAX (Promega), 10 mM TCEP ...
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bioRxiv - Molecular Biology 2020Quote: ... HaloTag-positive cells were labeled with Janelia Fluor 646 HaloTag fluorescent Ligand (Promega). GCaMP3-KI cells exhibited strong fluorescence when subjected to a flow cytometer maybe due to shear or/and mechanical stress (Fig ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... BICD2N or HaloTag alone were labeled with TMR-HaloTag ligand (Promega, Madison, WI) 18–24 h post-transfection then lysed in 100 μl lysis buffer [40 mM Hepes (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega). In situ hybridization was performed a combination of what has been described previously (50 ...
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bioRxiv - Biophysics 2023Quote: Labeling reactions: Proteins with HaloTag were labeled with JF646-HaloTag ligand (Promega # GA1120) by incubating the proteins with a tenfold excess of dye on ice for 0.5-1 h in the presence of 1 mM ATP ...
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bioRxiv - Cell Biology 2023Quote: ... BrdU/BrdC-labeled DNA was digested with 10 U/μl Exonuclease III (Promega) in buffer supplied by the manufacturer for 30 minutes at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... we labeled the cells with 2.5 µM HaloTag-Ligand (HTL) TMR (Promega #G8251) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxygenin labeled antisense probes were synthesized using SP6 or T7 RNA polymerase (Promega), and purified using LiCl precipitation.
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bioRxiv - Microbiology 2022Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of nsp4 RNA using mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of N RNA using mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... or purified LgBiT-substrate cocktail (Promega). After incubation with appropriate HRP-tagged secondary antibodies and chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... or purified LgBiT-substrate cocktail (Promega), the blots were imaged and processed with a FluorChem E (Protein Simple).
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Microbiology 2021Quote: ... Immunodetection was carried out using rabbit polyclonal anti-Halo antibody (Promega; 1:1000), mouse anti-HA antibody (Roche ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were either goat anti-mouse IgG HRP or goat anti-mouse IgG HRP (Promega; 1:10,000) respectively.
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bioRxiv - Cell Biology 2019Quote: ... Goat anti-rabbit IgG-HRP (W401B, Promega); Horse anti-mouse IgG-HRP (PI2000 ...
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bioRxiv - Biochemistry 2022Quote: ... or Goat anti-Rabbit IgG (W401B, Promega) secondary antibodies for 1 hour (h) ...
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bioRxiv - Neuroscience 2023Quote: ... or anti-goat (1:1,000; Promega #V805A)) for 1 h at RT while shaking ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-mouse IgG (Promega, 1:10,000), goat anti-rabbit IgG (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rabbit IgG (Promega, 1:20,000), goat anti-rat IgG + IgM (L+M ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were labeled with 100 nM Janelia Fluor 646 HaloTag ligand (Promega, GA1120) for 30 min ...
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bioRxiv - Plant Biology 2022Quote: ... A digoxigenin (DIG)-labeled antisense (AS) probe was synthesized using the T7 polymerase (PROMEGA), for hybridization on pre-parasitic stage 2 juvenile nematodes (ppJ2) ...
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bioRxiv - Biochemistry 2022Quote: ... A portion of cells were labeled with 100 nM HaloTag TMR ligand (Promega; G8252) and fluorescent colonies were identified using a TIRF microscope ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT antibodies were then purified from the verified serum by ammonium sulfate fractionation and then antigen affinity purification using recombinantly expressed HaloTag-NOCT (1-431) immobilized on HaloLink resin (Promega). The serum was purified over the immobilized NOCT column before elution with 5 M NaI and 1 mM ammonium thiocyanate ...
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bioRxiv - Molecular Biology 2019Quote: ... The natural templates include purified human (Promega), rhesus monkey (BioChain) ...
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bioRxiv - Neuroscience 2019Quote: ... and DNaseI-purified (Promega, Madison, WI, USA). cDNA was generated using the Superscript II protocol (Life Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... The PCR DNAs were gel purified (Promega Wizard SV Gel and PCR Clean-up System #A9282 ...
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bioRxiv - Genetics 2023Quote: ... Purified RNA was treated with DNase (Promega) at 37°C for 30 min ...