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Citations for Promega :
251 - 300 of 820 citations for Saporin Chicken Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Purified cysts were then resuspended in 75 μl Cell Lysis buffer (Promega) and sonicated for 2×10 seconds to break the cyst wall ...
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bioRxiv - Microbiology 2021Quote: ... RNAs were purified using the SV total RNA isolation kit (Promega USA) and resuspended in RNase-free water (Invitrogen USA) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Purified cysts were then resuspended in 75 μl Cell Lysis buffer (Promega) and sonicated for 2×10 seconds to break the cyst wall ...
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bioRxiv - Developmental Biology 2020Quote: ... Purified fragments were pooled and subcloned in pGEM-T Easy Vector (Promega). 16 clones for each sample were picked and sequenced ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid DNA was purified with a Wizard Plus SV Miniprep kit (Promega). Restriction enzyme digestions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific or NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was purified following the column-based purification protocol (A1460, Promega). The set of different primary antibodies used in the ChIP assays are mentioned below:
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bioRxiv - Immunology 2020Quote: ... Purified PCR products were ligated in pGEM-T easy vectors (Promega, USA) and transformed into Escherichia coli (DH5a strain) ...
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bioRxiv - Genomics 2020Quote: ... The MeDIP DNA was purified using the PCR cleanup kit (A1460, Promega).
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bioRxiv - Microbiology 2021Quote: Genomic DNA was purified using the Wizard Genomic DNA purification kit (Promega) then sequenced via Illumina sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... Those were purified by wizard gel and PCR clean-up kit (Promega), achieving a final concentration of 200 ng/µL ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA samples were purified using the RNeasy miniprep kit (Z3741, Promega) following the manufacturers’ instruction ...
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bioRxiv - Biophysics 2022Quote: ... Fusion protein was purified using HisLink Protein purification resin (Promega (Madison, WI) #V8821 ...
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bioRxiv - Genomics 2022Quote: ... The cDNAs were subsequently purified with ProNex magnetic beads (Promega, WI, USA) following the instructions in the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified bacmids were transfected into Sf9 cells (Spodoptera frugoperda) using FuGene (Promega) and baculoviruses amplified for two rounds before protein expression in Hi5 cells.
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bioRxiv - Genomics 2022Quote: ... purified by Wizard® SV Gel and PCR Clean-Up System (Promega), and labelled using nick translation protocol by (Kato et al. ...
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bioRxiv - Microbiology 2022Quote: ... The clarified soluble protein suspension was purified using MagneGST Glutathione Particles (Promega) according to manufacturer recommendations ...
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bioRxiv - Physiology 2023Quote: ... purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned into the vector pCR2.1 using the TOPO TA Cloning Kit (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting purified libraries were finally quantified using a Quantus fluorometer (Promega) and combined in equimolar amounts prior to paired-end sequencing (2 x 250-bp ...
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bioRxiv - Microbiology 2023Quote: The pABA plasmid was purified using PureYield™ Plasmid Miniprep System (Promega), starting with 5 mL of an overnight grown culture ...
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bioRxiv - Microbiology 2023Quote: When required PCR purified amplicons were ligated into pGEM-T Easy (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was purified using a Wizard genomic DNA purification kit (Promega). DNA was tagged and multiplexed with the Nextera XT DNA kit (Illumina) ...
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bioRxiv - Immunology 2022Quote: ... Released DNA fragments were purified by PCR product purification kit (Promega, A9282) and used to generate libraries ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA was purified from minipreps with QIAprep spin Miniprep Kit (Promega), and constructs were verified by sequencing (Microsynth).
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bioRxiv - Biophysics 2023Quote: ... the handles were purified using a PCR clean up kit (A9282, Promega).
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bioRxiv - Genomics 2023Quote: ... PCR products were purified via gel excision and column purification (Promega, A9282), followed by insertion into the BsmBI–digested lentiV2-hU6-evopreQ1 vector by Gibson assembly (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... and the subsequent PCR product was purified using Wizard SV Minicolumns (Promega). Double-strand RNAs (dsRNAs ...
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bioRxiv - Genomics 2023Quote: ... Individual reactions were combined for each sample and column purified (Promega, A9282). The purified products were then amplified by indexing PCR to add Illumina TruSeq adaptors and sample index sequences with the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was purified using a Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... samples were further purified using the ProNex Size-Selective Purification System (Promega). Concentrations were measured by Qubit dsDNA broad range assay ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Genomics 2023Quote: ... The cDNA was then purified with ProNex magnetic beads (Promega, WI, USA) according to the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Purified PCR products were inserted into pGEM-T Easy vector (Promega,USA).
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bioRxiv - Genetics 2023Quote: ... PCR products were gel-purified and cloned into pGEM-T Easy (Promega) for Sanger sequencing ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... Mcm2-7 was probed with polyclonal UM174 (gift from Bell lab, MIT - (Chen and Bell, 2011)) and anti-rabbit HRP conjugate secondary (Promega W401B), tubulin was probed with monoclonal anti-tubulin antibody (Sigma T5168 ...
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bioRxiv - Immunology 2019Quote: ... from C57BL/6J or MD4 mice that had been pre-treated with CK-689,CK-666 were added to COS-7 APCs expressing either the single-chain anti-Ig,𝜅 surrogate Ag or mHEL-HaloTag that was labeled with HaloTag tetramethylrhodamine ligand (Promega, #G8251). After the indicated times at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Raw PCR products were then used to generate digoxigenin-labeled RNA probes using a T7 RNA in vitro transcription kit (Promega / Life Technologies). RNA was ethanol precipitated and resuspended in water to analyze on a Nanodrop ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: COS-7 cells grown in 10 cm plates to 70–80% confluence expressing Halo-tagged K560 or KIF5B (KHC) were labeled with TMR-HaloTag ligand (Promega, Madison, WI) 18–24 h post-transfection then lysed in P12 buffer (as described above ...
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bioRxiv - Developmental Biology 2022Quote: ... DIG-labeled anti-sense probes (cmyb, spi1, lcp1, and hbae1) were made from the pGEM®-T Easy vector (Promega Corporation, A1360) containing the gene-coding sequences via in vitro transcription using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Biophysics 2023Quote: HeLa cells expressing each Halo-tagged chromatin remodeler were labeled with 5 nM Janelia Fluor® 549 (JF549) Halo-tag® ligand (GA1110, Promega) for 15 min at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega), cloned into the pGEM-T Easy vector (Promega) ...
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Relationship between True Digestibility of dietary Phosphorus and Gastrointestinal Bacteria of GoatsbioRxiv - Microbiology 2019Quote: ... The amplicons were purified with a PCR Clean-Up system (Promega, Madison, USA) by a purification kit (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified twice with a miniprep kit (PureYield™ Plasmid Miniprep System, Promega). Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E ...
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bioRxiv - Molecular Biology 2020Quote: ... purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) or MinElute® PCR Purification Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... approximately 5.2 µg of purified gDNA (spiked with 1% unmethylated lambda DNA, Promega) was sheared into fragment size of 200-300 bp using Covaris S220 ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA samples were then purified using the Wizard SV PCR purification kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The fragments excised from the gel purified by the DNA Purification kit (Promega) according to the maker instructions.
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bioRxiv - Plant Biology 2020Quote: ... Purified products were cloned into the pGEM®-T Easy Vector Systems (Promega) for Sanger sequencing.
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified using the Wizard PCR Preps DNA Purification System (Promega) and DNA sequencing was performed using primer 2569 ...