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Citations for Promega :
301 - 350 of 1090 citations for SARS Coronavirus Spike Glycoprotein S2 Mosaic C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Biochemistry 2023Quote: ... 200 ng of Trypsin/Lys-C mix (Promega) was added ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 μg of trypsin/Lys-C (Promega) on a rotisserie for 18 h at ambient temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2 μg of Trypsin/Lys C (V5071, Promega) was added into each 100⍰μl sample in 50⍰mM ammonium bicarbonate overnight at 37⍰°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were digested with Lys-C/Trypsin (Promega) at 37 °C overnight with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The proteases trypsin and Lys-C (Promega, V5073) were used to digest the LtEc proteins overnight at 37°C after reduction with 5 mM TCEP at 55°C and alkylation with 20 mM iodoacetamide at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
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bioRxiv - Synthetic Biology 2021Quote: All experiments were performed using Caulobacter crescentus NA1000 (also known as CB15N) and Escherichia coli DH5α and BL21 purchased from Promega. C ...
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bioRxiv - Biophysics 2019Quote: ... coli HB101 cells and isolated in quantity by using a Promega Wizard Plus Megaprep purification kit (Promega Corp., Madison, WI).
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bioRxiv - Systems Biology 2019Quote: ... 100 μg of proteins were precipitated by methanol-chloroform method and digested overnight at 37°C with 1 μg trypsin/Lys-C mixture (Promega Cat#V5073) 19 ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Biochemistry 2021Quote: ... and alkylated with iodoacetamide to a final concentration of 15 mM for 30 min at 37 °C in a dark environment with Lys-C (Promega, Cat # V1671) at a 1:100 (wt/wt ...
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bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 90% methanol/100 mM TEAB 10 times prior to on-column digestion with 1 ug Trypsin/Lys-C in 50 mM TEAB (47° C, 2 hours; Cat. #V5073, Promega, Madison, WI). The resulting peptides were eluted with sequential washes of 50 mM TEAB ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then digested overnight at 35ºC using Trypsin/Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade, Promega, Cat No: V5072); the enzyme-substrate ratio of 1:70) ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was performed in the S-Trap overnight at 37°C using Trypsin/Lys-C Mix Mass Spec Grade (Promega, Walldorf, Germany), followed by elution in 50 mM TEAB ...
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bioRxiv - Microbiology 2020Quote: ... Samples were digested with rLys-C (#V1671, Promega, France) ratio 50:1 (protein:rLysC ...
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bioRxiv - Genetics 2021Quote: ... Samples were then digested overnight with rLys-C (Promega), the peptides were cleaned on a Supelco Visiprep SPE Vacuum Manifold (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... Modified trypsin/Lys-C was from Promega (Madison, WI). Poros R2 and Poros Oligo R3 reversed-phase material were from Applied Biosystems (Forster city ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were digested first by Lys- C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Genetics 2022Quote: ... 0.5 µg of Trypsin/Lys-C Mix (Promega, U.S.A.) was added ...
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bioRxiv - Biochemistry 2022Quote: ... MS-grade trypsin/Lys-C mix (200 ng, Promega) in 25 mM Tris-HCl (pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... or a combination of trypsin and Glu-C (Promega); aliquots of etanercept proteins were digested respectively using trypsin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... or a combination of Glu-C and AspN (Promega). For the analysis of deglycosylated glycopeptides ...
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bioRxiv - Neuroscience 2023Quote: ... After overnight digestion using Trypsin/Lys-C (Promega, #V5073), supernatant was collected from the magnetic beads ...
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bioRxiv - Cell Biology 2023Quote: ... After overnight digestion with Trypsin/Lys-C mix (Promega), peptides were transferred ...
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bioRxiv - Microbiology 2023Quote: ... which were digested with 1 µg Lys-C (Promega) overnight at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... then with trypsin (Promega; 1:50, 37°C overnight). Samples were purified by solid-phase extraction (SepPak tC18 cartidges ...
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bioRxiv - Microbiology 2024Quote: ... and pBiT2.1-C[TK/SmBiT] were obtained from Promega. ALTO ...
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bioRxiv - Cancer Biology 2024Quote: ... and Trypsin/Lys-C Mix (Promega, Madison, WI, USA) at a 1:50 ratio in a ThermoMixer C (Eppendorf ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin/Lys C was purchased from Promega (Madison, USA). ITO slides were obtained from Delta technologies (Loveland ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 µg of trypsin/Lys-C mixture (V5073, Promega) added to each on-bead sample and set to rotate head-over-head overnight at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... protein digestion was performed by 1 µg of Lys-C (Wako) at 37 °C for 3 h following 1 µg of trypsin (Promega, Madison, WI, USA) at 37 °C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... After five-fold dilution with 50-mM ammonium bicarbonate, the sample was digested using Lys-C (Wako, Osaka, Japan) at 37°C for 3 h following trypsin (Promega, Madison, WI, USA) at 37°C for 16 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 500-ng of Trypsin/Lys-C Mix (Promega Cat#V5072) was added and allowed to digest overnight for ∼18-hr at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Digestion was performed overnight at 37°C with trypsin (Promega V5113 ...
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bioRxiv - Plant Biology 2020Quote: ... in 50 mM NH4HCO3 or Glu-C (sequencing grade, Promega) in 50 mM Tris/PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed using mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 8 overnight at 37 °C (Promega) at an estimated protein:enzyme ratio 1:100 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl2 and 500 ng Trypsin Lys-C (Promega). On bead digest was carried out overnight at 37°C while shaking at 200 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested 16 hours at 37°C with trypsin (Promega) and stopped by addition formic acid (FA ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...