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Citations for Promega :
51 - 100 of 985 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein ...
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bioRxiv - Developmental Biology 2021Quote: ... Spike-in RNA (polyadenylated kanamycin mRNA #C1381, Promega, USA) was added to the Cell Lysis Buffer with a final concentration 107 molecules/ml ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... an anchor-oligo(dT) primer (Table S1) and random hexamers (Promega). The reverse anchor and forward nested degenerated PGM primers derived from the oyster Crassostrea gigas and human Pgm-1 sequences were then used to perform the upstream amplification of cDNA fragments (see Table S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... The probes (Table S1) were 32P-labeled with T4 PNK (Promega) and purified using Oligo Clean and Concentrator columns (OCC-5 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with S1 nuclease (20 U/ml) (Promega M5761) in S1 buffer for 60 min at 37 °C
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... An N-terminal HALO tag (Promega) for fluorescent labelling was fused to the coding sequence through a GGSGGSLQ linker ...
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bioRxiv - Cell Biology 2022Quote: ... into pHTN Halo-Tag vector (Promega). mCherry-WT-p62 ...
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bioRxiv - Molecular Biology 2023Quote: ... Halo Tag Alexa Fluor 660 (Promega) was used ...
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bioRxiv - Biochemistry 2023Quote: ... RBD proteins in culture media were measured in Nano-Glo buffer (Promega N1110) supplemented with Nanoluciferase substrate and 11S protein according to the manual ...
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bioRxiv - Immunology 2019Quote: ... CHO-K1 cells (European Collection of Cell Cultures) were stably transfected with plasmids using FuGene (Promega), stable cell lines were created ...
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bioRxiv - Cell Biology 2020Quote: ... Expression plasmids (Table S1) were transiently transfected using Fugene6 (Promega, Madison, WI) according to the manufacture’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... and HHEX (NM_002729) attached to a FLAG tag at the 5’ end and HiBit tag (Promega, Madison, WI) at the 3’ end ...
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bioRxiv - Developmental Biology 2021Quote: ... purified genomic DNA (with 1% unmethylated lambda DNA spike-in, Promega) was sheared to a fragment size of 100 - 700 bp (primary size 250 bp) ...
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bioRxiv - Biophysics 2021Quote: ... and Halo-Tag (O4) ligand (Promega, P6741). To avoid non-specific interaction the glass chambers were washed with blocking buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... JF646-conjugated Halo Tag ligand (Promega, GA1120) was added to culture at a final concentration of 200 nM and cells were incubated for 30 mins before live-imaging ...
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bioRxiv - Biophysics 2022Quote: ... Then Halo-Tag (O4) ligand (Promega, P6741) were passed and incubated overnight at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... Halo Tag Alexa Fluor 488 Ligand (Promega) was diluted with moderate amount of PBS ...
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bioRxiv - Microbiology 2023Quote: ... Mouse anti-HiBiT tag mAb (30E5, Promega) and goat anti-mouse IgG H&L conjugated with Alexa Fluor 568 (Abcam ...
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bioRxiv - Biochemistry 2023Quote: ... with the C-terminal HiBiT-Tag (Promega), using the In-Fusion cloning kit ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... PUM2 RBD (aa 705-1049) were Flexi cloned into mammalian expression vector pF5K (Promega) that contained the MS2 coat protein RNA-binding domain and a V5 epitope tag at the N-terminus ...
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bioRxiv - Neuroscience 2019Quote: CHO-K1 cells (ATCC, cultured as described above) were transfected with rat TRAAK-GFP with FugeneHD (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo-tag protein (Promega), with the insertion of a 45-base linker (15 amino acids ...
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bioRxiv - Cancer Biology 2022Quote: ... Halo tag TMR Direct Ligand (Promega, cat # G2991) was used for staining of Halo tag transfected fixed cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Halo-tag (G9211) antibody was purchased from Promega. The C11orf53 antibody was produced in rabbit in house by using full-length C11orf53 recombinant protein as antigen.
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bioRxiv - Molecular Biology 2023Quote: ... rabbit polyclonal anti-Halo Tag antibody (G928A; Promega), and mouse monoclonal anti-GAPDH antibody (M171-3 ...
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bioRxiv - Molecular Biology 2024Quote: ... with Halo-Tag TMRDirect ligand (Promega, WI, USA), as per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... PFGE of total DNA either undigested or digested 15 min with S1 nuclease (Promega) was carried out as described by Freitas et al ...
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bioRxiv - Microbiology 2019Quote: ... we performed Pulse Field Gel Electrophoresis (PFGE) on S1 nuclease (Promega, Madison, WI, USA) digested DNA ...
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bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
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bioRxiv - Immunology 2022Quote: ... CHO-K1 or HEK-293F cells (European Collection of Authenticated Cell Cultures) were transfected with plasmids using FuGene (Promega), and Fc-secreting cells cloned ...
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Cytoplasm localized ARID1B promotes oncogenesis in pancreatic cancer by activating RAF-ERK signalingbioRxiv - Cell Biology 2019Quote: Halo tag (Halo pulldown and labeling kit, Promega Corporations) and Streptavidin bead (GE Health care Bio-Science ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
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bioRxiv - Zoology 2021Quote: ... 10μL Go-tag qPCR Master Mix (Promega, Wisconsin, USA), 0.2μL of forward primer ...
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bioRxiv - Cell Biology 2021Quote: ... To detect HALO tag 0.5µM Janelia Fluor 549 (Promega) dye or HaloTag® TMR Ligand (Promega ...
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bioRxiv - Biophysics 2023Quote: ... followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl of pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... NTD and Spike - was achieved for 24 h using ProFection Mammalian Transfection System (Promega) or adenovirus infection ...
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bioRxiv - Cell Biology 2021Quote: ... The Halo-Tag or NanoLuc genes were acquired from Promega and were genetically fused to the respective N- or C-termini.
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bioRxiv - Biochemistry 2023Quote: Halo tags were stained with HaloTag® Ligands TMRDirect (Promega), and SNAP tags were stained with SNAP-Cell® 505-Star (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction products were split into 10 μL aliquots and S1 nuclease 10x reaction buffer (Promega) was added to 1x concentration ...
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bioRxiv - Plant Biology 2020Quote: ... Magne® Halo Tag® Beads (Promega, USA, Catalog No G728A) were used to purify recombinant proteins according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid expressing the Halo tag (pFN21A) was purchased from Promega. The expression plasmids His12_PreScission _C1_C2B_MUN_C2C_tev_Halo and His12_PreScission _C1_C2B_MUN_tev_Halo (ΔC2C Munc13 ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... reference beads followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The NanoLuciferase (NLuc) and Halo-Tag genes were obtained from Promega and the plasmids expressing b2AR- ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...