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Citations for Promega :
151 - 200 of 5800 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and pMRXIH together with 3×FLAG tag or HaloTag7 (N2701, Promega, Madison, WI). DNA fragments encoding ATG2A (NM_015104.3 ...
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bioRxiv - Neuroscience 2023Quote: ... and dyes used were anti-Halo-Tag (Promega, cat. G928A, 1:200 dilution), FluoTag-X2-anti-PSD95-Sulfo-Cy5 (28)(NanoTag Biotechnologies GmbH ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Cancer Biology 2023Quote: A volume of 250 μl fraction was mixed with 500 pg of luciferase RNA spike-in (Promega #L4561) and lysed in 750 μl Trizol LS (Thermo Fisher #10296028) ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative PCR (qPCR) was performed with the primers listed in Table S1 and a GoTaq qPCR Master Mix (Promega, A6002) in a Mastercycler realplex2 thermocycler (Eppendorf) ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: The upstream regions of genes of interest were amplified by PCR with specific primers (Table S1) and cloned into the pGEM-T easy plasmid (Promega). After digestion with proper restriction enzymes (Table S1) ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana cDNA using gene-specific primers listed in Supporting Information Table S1 as a NcoI-KpnI fragment by High Fidelity PCR (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... VA or Mutated (M)-sequences with the corresponding complementary strands (Supplementary Table S1) and then cloned using SmaI restriction site upstream the basal SV40 promoter into pGL3-promoter vector plasmid (Promega), in the same strand (coding or template ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Genomics 2023Quote: Promoters were amplified from mouse genomic DNA using primers listed in table S1 and cloned into pGL3-Basic (E1751, Promega) using SacI and BglII.
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bioRxiv - Cancer Biology 2022Quote: ... Pull down was performed as described in the Halo tag technical manual TM342 (Promega). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... SEPT6-HALO was made by cloning the HALO tag from pFN21K (Promega cat# G2821) onto the N-terminus of SEPT6 from SEPT6-GFP-pLVX-IRES-puro ...
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bioRxiv - Biochemistry 2019Quote: ... containing no tandem repeats of rare codons was PCR amplified from Eh genomic DNA with the specific primers EhTopoIIfgSBamHI and EhTopoIIfgASXhoI (S1 Table) using High Fidelity Taq polymerase (Thermo Fischer Scientific, USA) and subcloned into pGEMT-Easy (Promega, USA) via TA ligation ...
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bioRxiv - Pathology 2019Quote: ... Then the full DNA-A of TYLCV-C85S was amplified using the primers listed in S1 Table and inserted into the pGEM-T Easy (Promega, USA) vector to produce pGEM-1A-C85S ...
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bioRxiv - Plant Biology 2022Quote: A 1531 bp segment upstream of the transcription start site was isolated from Arabidopsis genomic DNA with specific oligonucleotides (Supplementary Table S1) and cloned into the pGEM®-T Easy (Promega). After that ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Immunology 2022Quote: The ADCC activity of anti-SARS-CoV2 S IgG antibodies was determined using the ADCC Reporter Bioassay (Promega) as previously described (Dufloo et al. ...
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bioRxiv - Biochemistry 2021Quote: ... the aqueous supernatant was transferred to a new tube to which 250 pg of a luciferase control RNA spike-in (luc, Promega). RNA was precipitated overnight at −20°C by the addition of 1 volume of isopropanol and 2 μL of GlycoBlue (15 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... On odd days 30 μl of Cell TiterGlo 2 (Promega cat # G924A) was added to the remaining 40 μl culture and incubated 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with 2% of the GloSensor reagent (Promega, cat. # E1290). Relative luminescence units were recorded using a SynergyMx microplate reader (Biotek) ...
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bioRxiv - Genetics 2020Quote: ... cells were transfected with 50 ng/well of the psiCHECK-2 (Promega) construct using the FuGENE HD Transfection Reagent (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... we added 10μL Cell Titer Glo reagent diluted 1:2 (Promega, Inc.) to each well and mixed ...
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bioRxiv - Biochemistry 2020Quote: ... Electroporated Halo-CENP-T was labelled using the Halo Tag Oregon Green fluorescent ligand (Promega) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... the MBP-tag was cleaved off the fusion proteins by ProTEV Plus (Promega, Cat# V6102). Cleavage efficiency was typically ~70-90% at 30 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... and (ii) HALO tag was incubated with primary Anti-HaloTag® Monoclonal Antibody (Promega #G9211) diluted 1:1 000 with secondary antibody Anti-Mouse IgG (Fc specific ...
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bioRxiv - Genomics 2023Quote: ... we followed a CRISPR knock-in protocol to endogenously insert a HiBiT tag (Promega 88) at the 3’ terminus of the IFNAR2-S mRNA (Fig ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Genomics 2023Quote: ... 2×10e4 cells were plated in 96-well plates and CellTiter-Glo® Luminescent Cell Viability Assay (Promega) was used to quantify cell viability 48h after drug or vehicle treatment using a Tecan Infinite M Plex plate reader (Tecan ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Microbiology 2019Quote: ... cells were treated with MEK1/2 specific inhibitor U0126 (20 μM final; Promega). Transfections with siRNAs were performed using JetPRIME (Polyplus ...
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bioRxiv - Genetics 2020Quote: ... Cell growth after 4 days was measured using the CellTiterGlo 2 kit (Promega) and a GloMax luminometer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products amplified by gene-specific RT-qPCR primers listed in Supplemental Table S1 were cloned in pGEM-T Easy vector (Promega, Madison, Wisconsin, USA) prior to their sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... Barcodes were amplified using forward primer MWV 486 and a 5’-biotinylated reverse primer MWV 358 (Table S1) using standard PCR conditions with Gotaq G2 flexi DNA polymerase (Promega, Leiden, the Netherlands). The biotinylated PCR products were then hybridised to a pool of MagPlex-TAG microspheres (Table S3 ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Biophysics 2023Quote: ... was generated by PCR-cloning the HaloTag coding sequence (primers details in Table S1, Integrated DNA Technologies) from pFN21K HaloTag® CMV Flexi® Vector (Promega, Madison, WI), followed by ligation in place of the mApple sequence in the mApple-CD36-C-10 vector (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured via the HiBiT tag using Nano-Glo® HiBiT Extracellular Detection System (Promega) on a FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Biophysics 2023Quote: ... prior to incubation with the primary anti-Halo-Tag antibody (Mouse monoclonal antibody, Promega, Cat. No.: G9211). For incubation with the primary anti-RBPJ antibody (Rat monoclonal antibody ...
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Microbiology 2020Quote: ... was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500) and placed on a rocker for 15 min ...