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Citations for Promega :
101 - 150 of 1070 citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21(DE3) pLysS cells (Promega). Cells were grown in Lysogeny broth to an OD 600 of 0.5-0.7 and protein expression was induced with 0.5 mM IPTG for 20 h at 16 °C ...
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bioRxiv - Microbiology 2024Quote: ... coli strain JM109 (Promega; Madison, WI). JM109 harboring pJBA132 was routinely cultured in Miller’s Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2024Quote: ... coli KRX cells (Promega, Wisconsin, USA), and cultivation in 1 L Terrific Broth medium supplemented with 100 μg/l ampicillin ...
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bioRxiv - Cancer Biology 2024Quote: ... were cloned into pcDNA3.1-myc-His plasmid (Promega). For sequencing we used a sequence analyser (ABI Prism 3100 Avant ...
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bioRxiv - Microbiology 2023Quote: ... or the Magne-His purification system (Promega Corporation) for PlzARD-RD per manufacturer protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Halo-tag PUM1 RBD (aa 828-1176) and Halo-tag PUM2 RBD (aa 705-1050) were expressed from plasmid pFN18A (Promega) in KRX E ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo-tag protein (Promega), with the insertion of a 45-base linker (15 amino acids ...
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bioRxiv - Cancer Biology 2022Quote: ... Halo tag TMR Direct Ligand (Promega, cat # G2991) was used for staining of Halo tag transfected fixed cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Halo-tag (G9211) antibody was purchased from Promega. The C11orf53 antibody was produced in rabbit in house by using full-length C11orf53 recombinant protein as antigen.
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bioRxiv - Molecular Biology 2023Quote: ... rabbit polyclonal anti-Halo Tag antibody (G928A; Promega), and mouse monoclonal anti-GAPDH antibody (M171-3 ...
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bioRxiv - Molecular Biology 2024Quote: ... with Halo-Tag TMRDirect ligand (Promega, WI, USA), as per the manufacturer’s guidelines ...
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bioRxiv - Genomics 2020Quote: ... coli JM109 cells (Promega, Madison, WI, USA) and plasmid was isolated E ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 (DE3) pLysS cells (Promega, # L1195) and WARS was expressed in E ...
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bioRxiv - Microbiology 2022Quote: ... coli strains harboring pNL1.1 (Promega, Madison, WI) (see primers in Table S4 ...
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bioRxiv - Biochemistry 2019Quote: ... in Escherichia coli strain BL21(DE3) (Promega) and purified via glutathione-affinity column chromatography ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) pLyS (Promega, Madison, WI) and grown in Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2024Quote: ... coli collections using PureYield Plasmid Midipreps (Promega).
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bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
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Cytoplasm localized ARID1B promotes oncogenesis in pancreatic cancer by activating RAF-ERK signalingbioRxiv - Cell Biology 2019Quote: Halo tag (Halo pulldown and labeling kit, Promega Corporations) and Streptavidin bead (GE Health care Bio-Science ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
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bioRxiv - Zoology 2021Quote: ... 10μL Go-tag qPCR Master Mix (Promega, Wisconsin, USA), 0.2μL of forward primer ...
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bioRxiv - Cell Biology 2021Quote: ... To detect HALO tag 0.5µM Janelia Fluor 549 (Promega) dye or HaloTag® TMR Ligand (Promega ...
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bioRxiv - Biophysics 2023Quote: ... followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl of pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... the His-VqmA was purified using MagneHis Ni-Particles (Promega).
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Cell Biology 2024Quote: ... HIS-PLK1 used in ADP-Glo was from Promega (V2841). HIS-PLK1 used in Fig ...
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bioRxiv - Microbiology 2019Quote: Escherichia coli DH5α and pGEM-T easy (Promega) and pJET1.2/blunt (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... coli BL21(DE3) pLysS (Cat. # P9801, Promega, USA) competent cells ...
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bioRxiv - Molecular Biology 2019Quote: ... coli S30 Extract System for Linear Templates (Promega). Reactions containing 200 ng of mRNA and 0.1 mM of D-luciferin were carried out in 5 μL aliquots at 37°C for 15 minutes and the activity of in vitro synthesized luciferase was measured by VictorX5 (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... Escherichia coli BL21(DE3)pLysS cells (#L1195, Promega) were transformed with either pGEX-KG (containing the GST cDNA ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) were supplemented with 2.5 μM EL222 ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) were supplemented with 2.5 μM of Gluc-EL222 ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega).
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bioRxiv - Cell Biology 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Microbiology 2022Quote: ... coli JM109 (Competent Cells, Promega, cat. n° L2005) and recombinant clones were checked by sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain JM109 (DE3) was purchased from Promega. Liquid growth media and cultures were prepared in acid washed glassware or sterile plasticware to minimise metal contamination ...
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bioRxiv - Microbiology 2019Quote: ... After transformation of chemocompetent Escherichia coli JM109 (Promega) and plasmid isolation ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21(DE3)pLysS competent cells (Promega, UK) were selected for their suitability for transformation with a plasmid containing ampicillin resistance (pRSET/EmGFP plasmid ...
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bioRxiv - Bioengineering 2021Quote: ... coli S30 extract system for circular DNA (Promega) for RFP expression were supplemented with 2.5 μM EL222 (unless stated otherwise ...
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bioRxiv - Microbiology 2022Quote: ... Escherichia coli BL21(DE3)pLysS was from Promega.
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bioRxiv - Microbiology 2022Quote: ... coli using PureYield™ Plasmid Miniprep System (Promega) and suspended in milliQ water (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... coli S30 Extract System for Linear Templates (Promega). The 50-µl reactions were composed of kit components ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli S30 Extract System for Circular DNA (Promega). The second kit was only used for the T7 RNA polymerase circuit ...
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bioRxiv - Molecular Biology 2023Quote: ... coli S30 Extract System for Linear Templates (Promega), according to the manufacturer’s instructions for radiolabelled translation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... coli and isolated via mini-preps (Promega, A1330). Plasmid stocks for each GPCR had a 260/280 ratio of 1.7–1.9 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli BL21 (DE3) pLysS (Promega, WI, Madison, USA) strain harboring the plasmid pAflRT was grown to OD600 = 0.3 in LB medium with 25 μg/mL kanamycin at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cloning was performed in JM109 Escherichia coli (Promega). The plasmid is deposited at addgene under the name ...
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bioRxiv - Cell Biology 2021Quote: ... The Halo-Tag or NanoLuc genes were acquired from Promega and were genetically fused to the respective N- or C-termini.
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bioRxiv - Biochemistry 2023Quote: Halo tags were stained with HaloTag® Ligands TMRDirect (Promega), and SNAP tags were stained with SNAP-Cell® 505-Star (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... Plat-E cells were transfected with FuGENE™ HD (Promega) and transfection regent was incubated overnight before a media change ...