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Citations for Promega :
201 - 250 of 633 citations for Rubella virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... Clear lysates were treated with 5U RQ1 DNase and 5U RNase inhibitor (Promega) at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were sequentially treated with 12.6 μl of RNase-free DNase (Promega) and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units ...
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bioRxiv - Microbiology 2020Quote: ... 15µl of lysate were mixed with 50µl of Nano-Glo assay buffer (Promega) in a flat bottom white-walled luminescence plate ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were analyzed for luciferase activity using GloMax Discover Microplate Reader (Promega). For wild-type SARS-CoV-2 infection ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were collected and analysed using the dual-luciferase assay kit (Promega) following the manufacter’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were collected 24 hours post-transfection using Passive lysis buffer (Promega). Samples from three technical replicate transfections were measured in duplicate using a Zylux FB12 luminometer and the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... Epe1 fragments were translated in vitro using TNT T7-coupled reticulocyte lysate (Promega) with 35S-labeled methionine (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were analysed using the Dual-Luciferase Reporter Assay System (Promega, #E1910). Luminiscence was measured using a TECAN infinite M200-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysates were subjected to luciferase activity testing (Promega, Madison, WI, USA) 24 h later ...
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bioRxiv - Immunology 2021Quote: PP2A activity in cell lysates was measured using the Phosphatase Assay Kit (Promega). Endogenous free phosphate was removed using the columns ...
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bioRxiv - Developmental Biology 2021Quote: ... lysates were simultaneously analyzed for firefly luciferase activity and Renilla luciferase activity (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Lysate samples are generated by treatment of 1x Passive Lysis Buffer (Promega E1941) to 12 well plate cultures ...
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bioRxiv - Microbiology 2022Quote: ... Seventy five ug of each lysate were digested with sequencing-grade trypsin (Promega) 1:100 ratio for 12 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
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bioRxiv - Systems Biology 2022Quote: ... All lysates were further purified in the Maxwell RSC instrument (Promega, Madison, USA), using the Maxwell RSC blood DNA kit (n=376) ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation assays were performed using nuclease-treated rabbit reticulocyte lysate (Promega). Reactions were in 10 µl containing 2 µl RRL ...
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bioRxiv - Immunology 2023Quote: ... cell lysates were analyzed using the Dual-Luciferase® Reporter Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were used for luciferase assays (20 µL) with Luciferase assay system (Promega) and RNA extraction (20 µL ...
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bioRxiv - Biophysics 2023Quote: ... Luciferase activity of lysates was measured using the Dual Luciferase Assay Reagents (Promega) on a GloMax Multi microplate luminometer (Promega) ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in cell lysates collected 8 hours after transfection (Promega). To correct for transfection efficiency ...
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bioRxiv - Immunology 2023Quote: ... The luminescence of lysates was detected by Luciferase Assay System (Promega, Cat# E1500) and analyzed using a plate spectrophotometer ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). Capped and polyadenylated reporter mRNAs containing the first 135 nucleotides of NPR coding sequences (human ...
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bioRxiv - Molecular Biology 2024Quote: Rluc mRNA and Fluc mRNA were incubated with rabbit reticulocyte lysate (RRL, Promega) to generate the mRNA-ribosome complex ...
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bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Immunology 2021Quote: ... to each cell lysate and measuring the luminescence using a GloMax portable luminometer (Promega).
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer, Promega) was used to correct for transfection efficiency.
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bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase expression in the cell lysates was determined using a dual-luciferase kit (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Pathology 2021Quote: ... Fluorescence of the lysates was then analyzed using a multi detection plate reader (Promega Glomax Multi Detection System ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro translation was done using the TNT T7 Reticolucyte Lysate System (Promega Corp.), in the presence of radioactive 35S-methionine ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were harvested 24 h after infection in Reporter Lysis Buffer (Promega, E397A), lysed by freeze thawing at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501) using a Spectramax L luminometer with reagent injectors in technical triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... and combining equal volumes of cell lysates with the Bright-Glo™ Reagent (Promega). Co-transfection with a CMV-beta-galactosidase plasmid and bicinchoninic acid protein quantification assays were used for luciferase signal normalization.
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bioRxiv - Microbiology 2020Quote: ... Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer with dual injectors ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer with dual injectors ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Immunology 2022Quote: ... cell lysates were prepared and analyzed using the Dual-Luciferase Report Assay System (Promega).
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bioRxiv - Plant Biology 2022Quote: ... Cells were collected and lysate in BugBuster (EMDMillipore) with 5 ul RQ1 DNase (Promega). The supernatant was flowed through a column packed with Ni-NTA (QIAGEN) ...
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bioRxiv - Molecular Biology 2019Quote: ... whereas Control Firefly mRNA was provided with Rabbit Reticulocyte Lysate System (Promega Cat#L4960). In vitro reaction was translated for 15 minutes at 37°C and stopped with the addition of 100 µg of cycloheximide and stored on ice prior to luciferase assay.