Labshake search
Citations for Promega :
201 - 250 of 1711 citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
-
bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
-
bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 0.5 μM Halo-tag substrate CA-AlexaFluor488 (Promega) for 30 minutes at 37°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Halo-tag expressing cells were labelled with Halo-TMR ligand (Promega - G8251) following the manufacturers instructions for live cell labelling prior to fixation and permeabilization.
-
bioRxiv - Biophysics 2022Quote: ... and a goat anti-mouse tag: HRP 0.1 μL/mL (W402B - Promega).
-
bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
-
bioRxiv - Immunology 2020Quote: ... were pre-coated with recombinant luciferase (0.4μg/ml, Promega, #E1701) or spike protein (2μg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
-
bioRxiv - Molecular Biology 2020Quote: The plasmid for HaloTag (Halo) fusions was generated by cloning HaloTag (Promega) in the pBluescript SK (- ...
-
bioRxiv - Molecular Biology 2023Quote: The inserts were amplified from the HaloTag-PSMD3 fusion vector (Promega N2701). 10 ng of template DNA was combined with PrimeStarMax DNA Polymerase (Takara #R045Q ...
-
bioRxiv - Bioengineering 2023Quote: ... by using the PowerPlex® Fusion System 5C autosomal STR kit (Promega) as previously described 48 ...
-
bioRxiv - Cancer Biology 2023Quote: ... They were transfected with 1.5 μg NanoLuc-CDK9 fusion vector (Promega #NV2871) and 13.5 μg CCNT1 expression vector (Promega #NV2681 ...
-
bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
-
bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
-
bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...
-
bioRxiv - Synthetic Biology 2021Quote: ... We also included N-terminal and C-terminal HiBiT tags (Promega, Madison, WI) for quantification using luminescence ...
-
bioRxiv - Developmental Biology 2021Quote: ... the GST tag was cleaved from GST-TES-1 using ProTEV Plus (Promega), according to manufacturer’s instructions.
-
bioRxiv - Cell Biology 2022Quote: ... the cells were incubated with 1 nM Halo Tag TMR Ligand (G825A, Promega) at 37 °C for 2 h ...
-
bioRxiv - Cell Biology 2023Quote: ... and pMRXIH together with 3×FLAG tag or HaloTag7 (N2701, Promega, Madison, WI). DNA fragments encoding ATG2A (NM_015104.3 ...
-
bioRxiv - Neuroscience 2023Quote: ... and dyes used were anti-Halo-Tag (Promega, cat. G928A, 1:200 dilution), FluoTag-X2-anti-PSD95-Sulfo-Cy5 (28)(NanoTag Biotechnologies GmbH ...
-
bioRxiv - Biochemistry 2024Quote: ... the PCR products of the Strep-Flag-tag and NLuc (Promega, cat # N1091) were inserted into the pHLmMBP-8 vector (Addgene ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... we implemented a cell-free assay with purified recombinant luciferase (Promega) and luciferin (BrightGlo Luciferase Assay System ...
-
bioRxiv - Bioengineering 2020Quote: ... The standard curve was prepared by diluting recombinant ATP (Promega, P1132). For both assays ...
-
bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
-
bioRxiv - Biochemistry 2021Quote: ... recombinant bacmids were transfected into Sf21 cells using Fugene6 (Promega E2693). Supernatant containing viruses (V0 ...
-
bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease Inhibitor (1 uL/ml, Promega, Cat#N2511). The components of the high salt wash buffer are as follows ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant baculovirus was generated using FuGENE HD Transfection reagent (Promega) in Sf9 cells using Insect X-press (Lonza ...
-
bioRxiv - Immunology 2022Quote: ... into 293T cells in DMEM medium + 10% FCS using Fugene 6 (Promega) for pseudoviruses production ...
-
bioRxiv - Microbiology 2020Quote: ... These fusions were expressed from plasmids based on the pGEM-T backbone (Promega) named pOP plasmids (22) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... + 0.1 μg of NSD2 PWWP1-NanoLuc® Fusion Vector DNA (C-terminal) (Promega) using X-tremeGENE HP DNA Transfection Reagent (Sigma) ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK-293 were transfected with NLuc/target fusion constructs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... HiBiT fusion vectors were constructed using the parental vector pBiT3.1-N (N2361, Promega). To generate the HiBiT-VHL vector ...
-
bioRxiv - Molecular Biology 2023Quote: ... NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Final clones or pools were authenticated using the PowerPlex® Fusion System (Promega) performed at the Hartwell Center for Biotechnology at St ...
-
bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
-
bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
-
bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
-
bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
-
bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
-
bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
-
bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
-
bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
-
bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...