Labshake search
Citations for Promega :
1 - 50 of 265 citations for Respiratory Syncytial Virus A Glycoprotein G RSV A gG since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
-
bioRxiv - Physiology 2023Quote: ... and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega), in the presence or absence of indicated expression vectors using FuGene 6 transfection reagent (Promega) ...
-
bioRxiv - Microbiology 2021Quote: RSV VLPs were produced by FuGENE® 6 Transfection Reagent (Promega, E2691) transfection of DF1s at 50% confluence with 1 µg of viral plasmid ...
-
bioRxiv - Genetics 2024Quote: ... Virus was concentrated using PEG virus precipitation (Promega # V3011). In brief ...
-
bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... Bovine alpha-2-hs-glycoprotein was isolated from plasma (Fetuin, Promega, V4961), and monocyte differentiation antigen CD14 was recombinantly expressed in CHO cells (R&D systems ...
-
bioRxiv - Microbiology 2022Quote: ... RSV replication was evaluated by reading nano-luciferase activity with the Nano-Glo Luciferase Assay system (Promega) on a Tecan Spark plate reader ...
-
bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
-
bioRxiv - Microbiology 2020Quote: ... cell supernatants were harvested for RNA extraction with a Maxwell RSV Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). Then ...
-
bioRxiv - Biophysics 2020Quote: ... Virus infectivity was determined by luciferase production (Promega) after 48h using a Synergy2 Multi-Detection Microplate Reader (BioTek)
-
bioRxiv - Microbiology 2020Quote: ... virus replication was measured by nLUC assay (Promega) using a SpectraMax plate reader (Molecular Devices).
-
bioRxiv - Immunology 2020Quote: ... using Moloney murine leukemia virus reverse transcriptase (Promega) according to manufacturers’ instructions ...
-
bioRxiv - Pathology 2021Quote: ... we first prepared cDNAs from a total RNA sample isolated from an RSV-infected rice plant using the Reverse Transcription Kit as instructed (Promega, Madison, WI, USA). We then amplify the full-length RSV RNA3(- ...
-
bioRxiv - Cell Biology 2021Quote: ... conducted with Moloney murine leukemia virus reverse transcriptase (Promega) and random hexamers (Roche Applied Science ...
-
bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus MMLV reverse transcriptase (Promega) was performed according to the vendor’s protocol.
-
bioRxiv - Bioengineering 2022Quote: ... Virus growth was measured with NanoGlo Luciferase Assay System (Promega) and cell viability was measured with CellTiter-Glo 2.0 Cell Viability Assay (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... 200 U of Moloney murine leukemia virus reverse transcriptase (MMLV; Promega) in 30 mM Tris and 20 mM KCl (pH 8⋅3 ...
-
bioRxiv - Cell Biology 2020Quote: ... Virus DNA liposomes were prepared using FuGENE HD Transfection reagent (Promega) and DMEM ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega®) were added and incubated for 1h at 30°C ...
-
bioRxiv - Microbiology 2023Quote: ... Virus infectivity was measured by β-galactosidase activity (Promega, Madison, WI). Virus stocks were concentrated (20X ...
-
bioRxiv - Microbiology 2023Quote: Virus neutralization was measured using a β-galactosidase-based assay (Promega) with TZM.bl target cells (85) ...
-
bioRxiv - Cell Biology 2023Quote: ... and reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega). cDNA was amplified in a thermal cycler (GeneAmp PCR System 9700 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Moloney Murine Leukemia Virus Reverse Transcriptase RNase H-Point Mutant (Promega) was used for cDNA synthesis ...
-
bioRxiv - Molecular Biology 2019Quote: ... and cDNA was generated using the Moloney Leukemia Virus Reverse Transcriptase (Promega), both according to the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2019Quote: ... and reverse transcribed using Moloney murine leukaemia virus reverse transcriptase enzyme (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... virus stocks were first treated with 50 units/ml RQ1-DNase (Promega) for 1 h to remove any plasmid DNA ...
-
bioRxiv - Molecular Biology 2019Quote: ... and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(−) (Promega) using oligoT primer ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each RNA sample was reverse transcribed using murine leukemia virus reverse transcriptase (Promega) and random DNA hexamer primers ...
-
bioRxiv - Microbiology 2020Quote: ... Cells infected with Luciferase contaning virus were analyzed by Bright-Glo™ (Promega) using the manufacturers protocol ...
-
bioRxiv - Immunology 2019Quote: ... cDNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) with random hexamer primers ...
-
bioRxiv - Molecular Biology 2021Quote: ... and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Promega, Madison, WI), following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega, Madison, WI, USA) was performed according to the vendor’s protocol ...
-
bioRxiv - Microbiology 2019Quote: Virus infectivity and neutralization was measured using a β-galactosidase-based luciferase assay (Promega) with TZM-bl target cells ...
-
bioRxiv - Plant Biology 2020Quote: ... First-strand cDNAs were synthesised using Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, www.promega.com). The quantification of gene expression was made by qRT-PCR ...
-
bioRxiv - Genetics 2020Quote: ... Total RNA (0.5 µg) was reverse transcribed with mouse myeloblastosis virus reverse transcriptase (Promega) under standard conditions using hexanucleotide random primers according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... and then used for reverse transcription with avian myeloblastosis virus (AMV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The virus-induced CPE was assessed using the Viral ToxGlo™ assay (Promega #G8943) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
-
bioRxiv - Immunology 2021Quote: ... Magne Protein G beads (Promega) were used to purify the antibodies according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
-
bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
-
bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.