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Citations for Promega :
601 - 650 of 1395 citations for Recombinant Human UBE2V2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... proteins were reduced and alkylated (DTT/Iodoacetamide) before digest with Trypsin (Promega) and desalting of peptides using C18 material (Sola ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested for 3h at RT with 1μg LysC (Promega) and then digested overnight at 37°C with 2μg of Trypsin ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then subjected to digestion with trypsin (Promega; Madison, WI, USA) overnight at 37°C at 50:1 protein:trypsin ratio ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Genomics 2022Quote: ... protein was digested with Trypsin Gold (Catalog No. PRV5280, Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio for 16 hours at 37 □ ...
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bioRxiv - Genomics 2022Quote: ... the proteins were digested with sequence-grade modified trypsin (Promega, Madison, WI) at a substrate/enzyme ratio of 50:1 (w/w ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Microbiology 2022Quote: ... The clarified soluble protein suspension was purified using MagneGST Glutathione Particles (Promega) according to manufacturer recommendations ...
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bioRxiv - Systems Biology 2022Quote: ... The washed beads-proteins were then released into the trypsin (Promega, V5111)-containing deepwells at a 50:1 (w/w ...
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bioRxiv - Systems Biology 2022Quote: ... LC-MS performance was quality controlled with yeast protein extract digest (Promega). MS raw data was stored and managed by openBIS (v20.10.0 ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI). HRP conjugated anti-beta antibody (sc-47778 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight with Sequencing Grade Modified Trypsin/Lys-C (Promega) at 370C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50 μg total protein was then digested with 1 μg trypsin (Promega) overnight at 30 °C in the presence of 0.5% SLS ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were revealed using an ECL western blotting substrate (Promega, USA) and images were digitized using a Fusion FX (Vilber Lourmat ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described71 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested overnight in 25 mM ammonium bicarbonate with trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were revealed using an ECL Western Blotting Substrate (Promega, USA).
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bioRxiv - Bioengineering 2023Quote: ... Proteins were cleaved via trypsin and Lys-C endoproteinase (Promega, Madison, WI), at a ratio of 1:50 enzyme to protein overnight (12-16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were digested overnight with 1ul of 0.1μg/ul LysC (Promega) and 2ul of 0.4μg/ul trypsin (Pierce ...
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bioRxiv - Biochemistry 2023Quote: GsaB-modified GsaA precursor protein was digested with trypsin (Promega, sequencing-grade) at 37 °C for 20 min. ...
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bioRxiv - Microbiology 2023Quote: ... and so proteins were instead digested using sequencing grade gluC (Promega Corporation) added to the solution in a 1:20 mass ratio ...
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bioRxiv - Biophysics 2023Quote: ... The vectors expressing other HaloTag-conjugated proteins were: H2B (pFC15A backbone, Promega) 15 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then in-gel digested using modified trypsin (sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were digested with Trypsin/LysC mix (Promega, Wisconsin, WI, USA) at a 1:50 (w/w ...
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bioRxiv - Immunology 2023Quote: ... Protein was digested with either trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a mass ratio of 1:30 overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Biochemistry 2023Quote: ... A whole-cell protein extract of Saccharomyces cerevisiae (Promega, V7341, lot 434786) was deployed ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested in-gel using trypsin (600 ng/band, Gold, Promega), as previously described [29] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Treated proteins were then digested with sequencing grade modified trypsin (Promega, V5111) and incubated overnight (37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... The TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260) was used to generate Halo-tagged proteins starting with pIX-HALO plasmids as described previously (Mukherjee et al. ...
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bioRxiv - Plant Biology 2023Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... proteins isolated by gel electrophoresis were digested with trypsin (Promega, Wisconsin, USA) in 100 mM NH4HCO3 (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The proteins were subsequently digested overnight with molecular grade trypsin enzyme (Promega) using a ratio of 1:50µg (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... subjected to protein precipitation using Chloroform/Methanol and digested with trypsin (Promega). Desalted and acidified peptides (0.1% TFA ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein-bead complexes were digested with a mixture of trypsin:LysC (Promega) at a 1:20 ratio overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... before proteolytic digestion with LysC (enzyme to protein ratio 1:100, Promega) for 3 h and with trypsin (1:25 ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...