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Citations for Promega :
351 - 400 of 1395 citations for Recombinant Human SH2D1A Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... proteins were trypsinized overnight (Sequencing Grade Modified Trypsin, Promega, cleaves C-terminal to lysine and arginine residues ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A standard curve of HiBiT Control Protein N3010 (Promega) was diluted in PLB+PI at concentration ranging from 0.01 to 2 nM ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected with ECL Western Blotting Substrate (Promega) or SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
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bioRxiv - Physiology 2019Quote: ... Proteins were co-precipitated with 1 µg trypsin (Promega) overnight at −20 °C in 1 ml methanol ...
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bioRxiv - Plant Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analysed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with 300ng trypsin (Trypsin Gold, Promega) in 3µl 50mM ammonium bicarbonate followed by incubation at 37°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were digested with 0.25µg of Trypsin/LysC (Promega) at a 1:100 enzyme/substrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS ...
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bioRxiv - Cell Biology 2022Quote: ... Complexed proteins were eluted in SDS Elution Buffer (Promega) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with 1) chymotrypsin (Promega, Madison, USA), 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were in-gel digested with trypsin (Promega), five fractions per sample ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.
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bioRxiv - Microbiology 2023Quote: ... enriched proteins were eluted by adding 1µg trypsin (Promega) and incubation for 30min at 30°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the HiBiT Control Protein (20 µM, N3010, Promega) as a standard.
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested using sequencing grade modified trypsin (Promega) with a ratio of 1:50 (enzyme:protein ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were digested with 1µg Trypsin sequencing grade (Promega) for 14h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Proteins were subsequently digested with 15μg Trypsin Gold (Promega) in 50mM ammonium bicarbonate (Sigma-Aldrich ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Eluted proteins were then treated with PNGase F (Promega) according to manufacturer’s protocol and analyzed with SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with trypsin (Promega) overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were in-gel digested with trypsin (Promega, V5113). Tryptic peptides were purified by STAGE tips prior to LC-MS/MS measurements ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested with 500 ng of trypsin (Promega) and LysC (Wako Chemicals) ...
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bioRxiv - Microbiology 2024Quote: ... and proteins were digested with LysC and trypsin (Promega) in two steps with a final enzyme-to-protein ratio of 1:25 ...
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bioRxiv - Genetics 2024Quote: ... A 1:10 ratio (enzyme: protein) of Trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested on-beads with Trypsin (Promega) with ratio enzyme:proteins 1:20 in 50 mM ammonium bicarbonate and performed overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested with 0.1 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested overnight with 0.63 µg trypsin (Promega). Tryptic digestion was stopped by adding formic acid to a final concentration of 1.8% (v/v) ...
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bioRxiv - Genetics 2023Quote: ... Eluted proteins were digested with sequencing-grade trypsin (Promega). Peptide mixtures were separated using Evosep One (Evosep Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) using 2 μg Plasmid DNA per 50 μL expression reaction ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Microbiology 2021Quote: ... DENV2 structural proteins were cloned into expression vector from Promega in frame with a C-terminal Flag tag ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Proteins were digested using MS grade trypsin (Promega Trypsin Gold) in a ratio of 1:50 for 12 hrs ...