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Citations for Promega :
551 - 600 of 1395 citations for Recombinant Human SERPINA4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing grade, Promega), as previously described 58 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted from beads using partial on-bead trypsination (Promega) followed by a second digestion ...
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bioRxiv - Genetics 2024Quote: ... Proteins were immediately solubilized in 8 M urea and tryptic (Promega) digestion was performed overnight at 37°C with shaking followed by desalting ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Protein digestion was carried out with sequencing grade modified trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Immunology 2023Quote: Proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the purified protein was incubated with 50 µM Halo-Alexa488 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...
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bioRxiv - Molecular Biology 2019Quote: Full length 3’-UTR of human (P)RR and LDLR were cloned into the luciferase reporter vector (Promega). Mutant 3’-UTR luciferase reporter vectors were generated by PCR using site-directed mutagenesis technique ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Halotag fusion proteins were labelled with Janelia Fluor 646 Halotag Ligand (Promega) for live cell imaging ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were in-gel digested with trypsin (Promega, Madison, WI, USA), and the resulting peptide mixtures were processed on StageTips (Rappsilber ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 μg of protein was digested with sequencing grade trypsin (Promega) at 1:50 trypsin:sample protein amount for 14 hrs on a ThermoMixer at 37°C and 600 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant protein was then digested overnight with sequencing grade trypsin (Promega). The trypsin ...
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bioRxiv - Plant Biology 2019Quote: ... Chemiluminescent protein detections were conducted with ECL Western Blotting Substrate (Promega, W1001), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... Trypsinzation was achieved by incubating eluted protein with excess trypsin (Promega V5111) at 37°C overnight (16-18 hours) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then subjected to digestion with trypsin (Promega; Madison, WI, USA) overnight at 37°C at 50:1 protein:trypsin ratio ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated proteins were digested overnight with sequencing grade modified trypsin (Promega). The resulting peptides were resolved on a nano-capillary reverse phase column (PicoFrit column ...
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bioRxiv - Plant Biology 2021Quote: ... TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were in gel-digested overnight with sequencing grade trypsin (Promega). The resulting tryptic peptides were desalted using in-house C18 disks ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Neuroscience 2021Quote: ... The binding proteins were eluted by trypsin (Promega Corporation, Madison, WI, USA) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was performed by overnight digestion with trypsin sequencing grade (Promega).
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with 500 ng of sequencing-grade modified trypsin (Promega), and the resulting peptides were resolved on a nano-capillary reverse phase column ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested with 0.2 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Physiology 2022Quote: ... and proteins therein were in-gel digested with trypsin (Promega, Dübendorf, Switzerland), and resulting peptide mixtures were processed on STAGE tips and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using 100 μL of Passive Lysis Buffer (Promega). 20 μl of the supernatant were used to measure the LUC and RLUC activity using a Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... followed by protein digestion with sequencing grade modified trypsin (Promega, cat. V5111) at a protein-to-enzyme ratio of 50:1 at 37℃ overnight ...
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bioRxiv - Microbiology 2022Quote: ... 300 µg of protein per sample were treated with Trypsin Gold (Promega) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... together with molecular weight marker (Broad range molecular weight protein markers, Promega) was added to a 15% SDS-PAGE gel and separated under 200 volts ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was extracted using a passive lysis buffer (Cat# E1910, Promega). LUC activity was measured using a GloMax®20/20 Luminometer (Cat# E5311 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were then digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA) as described previously (Khusainov et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Proteins were reduced and digested with sequence-grade modified Trypsin Gold (Promega). Peptides were eluted with ammonium bicarbonate and acidified peptide mixtures from each biological replicate were analyzed in technical triplicates by nanoflow reverse-phase liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...