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Citations for Promega :
301 - 350 of 8272 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... stored at 4°C and assayed the following day for lactate dehydrogenase (LDH) activity as a measure of cell death using a Cytotox 96® Non-radioactive Cytotoxicity Assay (Promega, Madison, Wisconsin, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following day cells were trypsinized and seeded in 384-well white plate (20 µl/well) in DMEM F12 (no phenol red, 4% FBS) +/-HaloTag® NanoBRET™ 618 Ligand (1 µl/ml, Promega) and +/-compounds (DMSO concentration in each sample was kept the same) ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 uL/ml; Promega, Madison, WI; Cat#N2511). These washes were followed by an additional wash with 500 μl each of both wash buffer and high salt wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease inhibitor (1 ul/ml; Promega, Madison, WI; Cat#N2511) with a mechanical homogenizer followed by addition of TURBO DNase (2 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Systems Biology 2020Quote: The Human cell lysate was obtained commercially (Promega) and the yeast digest was prepared as previously described23 ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and were subsequently stained with 100 nM Halo Ligand JF549 (Promega, product number GA1111) and 100 nM Halo Ligand JF646 (Promega ...
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bioRxiv - Microbiology 2021Quote: ... NbALFA coupled to Alexa Fluor 647) or HaloTag® Fluorescent Ligand (Promega, Walldorf, Germany) coupled to Alexa Fluor 647 (STORM and MINFLUX ...
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bioRxiv - Cell Biology 2021Quote: ... The degradation of NCAPH2-HALO was triggered by adding HaloTag® PROTAC Ligand (Promega) at 1μM for 16 hours.
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bioRxiv - Biochemistry 2022Quote: ... The addition of HaloTag fluorescent ligands were added according to the manufacturer’s protocol (Promega). After incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Neuroscience 2020Quote: ... 1U/uL recombinant RNAsin(Promega), 10mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNasin Ribonuclease inhibitor (Promega), and SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and recombinant RNasin inhibitor (Promega). The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and ribonuclease (RNasin Recombinant, Promega) at concentrations of 1:1000 (sucrose buffer ...
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bioRxiv - Biochemistry 2020Quote: ... Electroporated Halo-CENP-T was labelled using the Halo Tag Oregon Green fluorescent ligand (Promega) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... we stained the TALE-TF with 1.25 μM Halo-TMR-ligand after manufacturer protocol (Promega) in cells grown with Auxinole (Aobious ...
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bioRxiv - Biophysics 2022Quote: ... and incubated with a 20 µg/ml solution of the HaloTag amine (O4) ligand (Promega) overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Fisher) containing 4% FBS with or without HaloTag NanoBRET 618 Ligand (Cat. No. PRN1662, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... HaloTag staining was conducted with the HaloTag TMR Ligand according to the manufacturer’s instructions (Promega). Confocal images were acquired with a Zeiss ConfoCor 2 (63× ...
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bioRxiv - Cell Biology 2023Quote: ... mG constructs were labelled by adding HaloTag® Oregon Green® Ligand (Promega, Chilworth, UK) to cells at a final concentration of 1 μM in the culture media and incubated 15 minutes (37°C ...
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bioRxiv - Biochemistry 2024Quote: ... HaloTag® polyclonal antibody (G9281) and TMRDirect™ fluorescent ligand (G2991) were purchased from Promega. AcTEV protease (#12575015 ...
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bioRxiv - Biophysics 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® Biotin Ligand (Promega, 200-fold dilution) in PBS supplemented with 5% NGS overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Systems Biology 2019Quote: Cells were lysed and recombinant proteins were isolated using Magne® HaloTag® Beads (Promega) as previously described (15) ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Cancer Biology 2024Quote: ... were cloned into pcDNA3.1-myc-His plasmid (Promega). For sequencing we used a sequence analyser (ABI Prism 3100 Avant ...
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bioRxiv - Microbiology 2023Quote: ... or the Magne-His purification system (Promega Corporation) for PlzARD-RD per manufacturer protocol ...
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bioRxiv - Biophysics 2020Quote: ... We performed smFISH with additional staining of TALE-TF with 1.25 μM Halo-TMR-ligand (Promega) and of membrane with 10 μg/ml Lectin-FITC (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... and zyxin were fluorescently labelled by incubation with Janelia Fluor 646 HaloTag ligand (JF646, Promega, GA1121) at a 1:2 molar ratio on ice for 2 hours ...
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bioRxiv - Biophysics 2022Quote: ... The HaloTag was labeled with a ligand covalently attached to the fluorescent dye tetramethylrhodamine (Promega Corp.) before measurement ...
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bioRxiv - Cell Biology 2023Quote: ... CHMP4B signal was visualized using far-red HALO ligand according to manufacturer recommendations (Promega catalog GA1120). Cells were processed as above for imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The APTES-coated coverslip was assembled into a flow channel and NH2-O4-Halotag ligand (Promega) was immobilized on to the coverslip through glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Halo-BicD2 (FL or truncated) was labeled by HaloTag® Alexa Fluor® 660 Ligand (Promega) by mixing protein solution with the dye at 1:2 molar ratio for 30-60 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: The initial HaloTag-based APMS (HaloMS) was performed using a commercially available HaloTag ligand plate (Promega, HaloLink 96 Well Plate ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.625 μl of recombinant RNasin (Promega), 1 μl of random hexamer primers (Promega ...
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bioRxiv - Physiology 2019Quote: ... 100 U/mL recombinant RNasin (Promega), 100 μg/mL cycloheximide ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2U/μL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Developmental Biology 2019Quote: ... Tagged-β-Catenin and Tagged-β-Catenin mutants were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...