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Citations for Promega :
1 - 50 of 1321 citations for Recombinant Human Lysyl Oxidase Like 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Microbiology 2023Quote: ... Transcend Biotin-Lysyl-tRNA (Promega) was incorporated in reactions so that reaction products could be identified using western blotting as above ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
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bioRxiv - Immunology 2021Quote: ... Transcend™ Biotin-Lysyl-tRNA (Promega, Cat.L5061), RNasin® Ribonuclease Inhibitors (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested with 2 μg of lysyl endopeptidase (Wako) at RT for overnight and were further digested overnight with 2 μg trypsin (Promega) at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Lysyl endopeptidase (Wako, Japan) and trypsin (Promega, USA) were added at a 100:1 ratio of sample protein:enzyme (w/w ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin and Lysyl endopeptidase (Promega) in a buffer containing 40 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with trypsin and Lysyl endopeptidase (Promega) in a buffer containing 40 mM ammonium bicarbonate ...
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bioRxiv - Immunology 2020Quote: ... Trypsin-Like and Caspase-Like Cell-Based Assays (Promega), according to manufacturer’s instructions (Moravec et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Trypsin-Like and Caspase-Like Cell-Based Assay (Promega) and GloMax® Discover microplate reader (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... and digested with trypsin and lysyl endopeptidase (Promega, USA) in a buffer containing 40 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteasome activities (chymotrypsin-like, trypsin-like and caspase-like) were assayed using the Proteasome-Glo kit (Promega, USA) in 384-well plates for luminescent assays ...
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bioRxiv - Molecular Biology 2024Quote: ... A two-stage enzymatic digestion with 2 hr lysyl endopeptidase (FUJIFILM Wako Chemicals U.S.A. Corporation) and o/n trypsin (Promega) was then performed to generate the crosslinked peptides.
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bioRxiv - Cell Biology 2023Quote: Cell-based ProteasomeGlo™ chymotrypsin-like and trypsin-like assays (Promega) were performed according to manufacturer’s instructions (Moravec et al ...
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bioRxiv - Systems Biology 2023Quote: ... 0.1 µg Lysyl Endopeptidase (FUJIFILM Wako) and 0.01% ProteaseMaxTM (Promega) in 20 mM ABC was added ...
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bioRxiv - Cell Biology 2022Quote: Three major proteasome activities (chymotrypsin-like trypsin-like, caspase-like) were measured using the Proteasome-Glo Cell-Based Assay Kit (G1180, Promega) as described by the manufacturer ...
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bioRxiv - Genomics 2020Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Systems Biology 2023Quote: ... Lysyl endopeptidase LysC (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) were added in a 1/100 enzyme to substrate ratio to digest the samples in a 96-well plate at 37 °C under constant agitation at 200 rpm overnight ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Cell Biology 2021Quote: ... trypsin-like and caspase-like activities were measured separately using the relevant substrates from Promega (Suc-LLVY-Glo ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... 10ng/mL of recombinant human FGF (Promega Corporation, Madison, WI), and 1µM dexamethasone (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Systems Biology 2023Quote: ... washing, and digestion with lysyl endopeptidase (Lys-C, Wako) and sequencing-grade trypsin (Promega). The capture and digestion steps were performed with a Versette liquid handling robot (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Proteasome-Glo Chymotrypsin-like assay (Promega G8621) was used for proteasome assays ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by 0.5 µg lysyl endopeptidase (LysC) (Fujifilm-Wako) and 0.5 µg Trypsin (Promega) at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako) and 1 μg trypsin (Promega) overnight at 37°C on a shaking incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Biochemistry 2023Quote: ... We employed a two-step digestion process (involving lysyl endopeptidase, from FUJIFILM Wako Chemicals U.S.A. Corporation, followed by trypsin, Promega) in-solution to produce the crosslinked peptide pairs.
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bioRxiv - Genetics 2024Quote: ... and Trypsin-Like Cell-Based Assays (No. G1180, Promega, USA), following the method proposed in a previous study [54] ...
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bioRxiv - Cancer Biology 2024Quote: ... We used Proteasome-Glo™ Chymotrypsin-like Assay (Promega G8621) and calculated the proteasome enzymatic activity in accordance with manufacturer recommendations ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with 0.5ug of lysyl endopeptidase (Wako) at room temperate overnight and were further digested overnight with 1ug trypsin (Promega) at room temperate ...
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bioRxiv - Physiology 2024Quote: ... Protein digestion was performed with Lysyl Endopeptidase (enzyme:substrate 1:100, 125-05061, Wako Pure Chemical Industries) and trypsin (enzyme:substrate 1:50, Promega V5113) overnight at 37°C with agitation (1,000 rpm) ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Developmental Biology 2021Quote: The Promega Proteasome-Glo Chemotrypsin-like assay (Promega cat. no. G8660) was used following the manufacturer’s protocols ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were digested with 0.5 µg of lysyl endopeptidase (Wako) at room temperature for 4 hours and were further digested overnight with 1 µg trypsin (Promega) at room temperature ...