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Citations for Promega :
351 - 400 of 667 citations for Recombinant Human Long Arg3 Insulin like Growth Factor I since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 3) goat anti-human H+L (Promega, W403B) at 1:3,000 dilution ...
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bioRxiv - Biochemistry 2020Quote: ... Human GSK-3β was purchased from Promega (V1991).
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bioRxiv - Genomics 2020Quote: ... and human gDNA (Promega, San Luis Obispo, CA) was added to reach a total input of 3 μg/130uL for fragmentation and library prep ...
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bioRxiv - Biochemistry 2021Quote: ... and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Beta cell death was determined in islet graft sections stained for insulin or glucagon using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method (Promega, Madison, WI) (20 ...
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bioRxiv - Genomics 2019Quote: ... Residual genomic DNA was digested by DNase I treatment (Promega, Cat No-M6101) and 200ng of RNA was reverse transcribed using AMV Reverse Transcriptase (Promega ...
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bioRxiv - Genomics 2022Quote: ... Each 5 μL fraction sample was treated with DNase I (cat #M6101, Promega) for 1 h at 37°C and then diluted 1:20 in 50 μg/mL yeast tRNA (cat #Am7119 ...
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bioRxiv - Developmental Biology 2022Quote: ... After removing the residual genomic DNA with the DNase I Kit (Promega, M6101), 500 ng of total RNA was reverse-transcribed into cDNAs using the PrimeScript RT Reagent Kit (TaKaRa ...
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bioRxiv - Genomics 2021Quote: ... followed by treatment with RNase-free DNase I (Cat. no. M6101, Promega, WI), according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2020Quote: ... followed by adding 200◻U of RQI RNase-free DNase I (Promega) for 1◻min ...
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 0.5 μg of RNAs were then digested by DNAse I (Promega, M6101) at 37°C for 50 min to remove the genomic DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 micrograms from each sample were DNAse I treated (Promega, Madison, WI) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... pCI Luc contains firefly luciferase cDNA cloned MluI / Xba I in pCI (Promega). pNLENG1-ES-IRES (a gift of D.N ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNA samples were treated with DNase I (Promega, Madison, WI, USA) and purified using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... on-membrane DNase I digestion was performed according to the manufacturer’s protocol (Promega). The integrity of the total RNA was assessed using spectrophotometric analysis using NanoDrop 2000/2000c ...
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bioRxiv - Plant Biology 2024Quote: ... and DNase I was added to digest the genomic DNA (Promega, no. Z3100) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... DNAse digestion was performed using RQ1 DNase I and RQ1 buffer (Promega M610A) for 30min at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Pellets were reconstituted in RNAse-free water and treated with DNase-I (Promega ##) as per manufacturers’ instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified with MagneGST™ Protein Purification System (www.promega.com/protocols/; Promega, Madison, WI, USA).
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bioRxiv - Immunology 2023Quote: ... 10µg of recombinant C5AP and PRGA was trypsinized with 1µg of trypsin (Sequencing Grade Modified Trypsin, Promega) for 15 min at 37°C and the trypsin activity was inhibited by incubating at 100°C for 5 min ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Microbiology 2021Quote: ... Growth inhibition was tested as above except cultures were grown in 7H9/OADC/glycerol medium and CellTiterBlue (Promega) was used as a measure of cell viability ...
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bioRxiv - Cancer Biology 2023Quote: Intracellular ATP levels representing cell growth were measured using the Cell Titer-Glo Luminescent Cell Viability Assays (Promega). ATP concentrations were normalized by cell number ...
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bioRxiv - Cancer Biology 2021Quote: ... and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study ...
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bioRxiv - Microbiology 2020Quote: ... insert (NRP1, native human ORF in pF1K, FXC23812, Promega): 5’-atagggcgaattcggtagtaaggagcgatcgc-3’ (fwd ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Biochemistry 2022Quote: ... human GR and MR were cloned into pACT (Promega) as N-terminal VP16 fusions using HiFi assembly (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... A cDNA encoding human KIF1A was purchased from Promega Japan (Promega Japan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Molecular Biology 2021Quote: ... or goat anti-Human IgG (H+L) (Promega, W4031) antibodies were added for 30 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... to 100 µl add 15 µl DNase I and 1.5 µl RNASin Plus (Promega) before use) ...
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bioRxiv - Systems Biology 2022Quote: ... and RNA (1.5μg) was treated with RQ1 Dnase I (Promega; 1 U/µg RNA). cDNA was synthesized with a qPCRBIO high-quality cDNA synthesis kit ...
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bioRxiv - Genomics 2021Quote: ... Total nucleic acid was then treated with DNase I as recommended (20 units; Promega), re-extracted with phenol/Sevag ...
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bioRxiv - Genomics 2021Quote: ... DNA collected from the DNase I digestion was quantified using a Quantus Fluorometer (Promega), and 10 ng was used to generate the libraries ...
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bioRxiv - Microbiology 2020Quote: One μg of total RNA was treated with 0.4 units of DNase I (Promega) and 0.2 units of Turbo-DNase (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... SYBR Green I nucleic acid gel stain and Go Taq Hot Start Polymerase (Promega). qRT-PCRs were carried out in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Gel extracted products were ligated into the pGEM-T Easy Vector System I (Promega). Ligation products were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... Extracted RNA was treated with DNase (RQ1 RNase-free DNase I, Promega, Beijing, China) to remove traces of genomic DNA ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was obtained from 1 μg of total RNA pretreated with DNase I (Promega) by reverse transcription reaction using ImProm-II(TM ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Microbiology 2020Quote: ... and clones of the recombinant vector was obtained using Wizard® Plus SV Minipreps DNA Purification kit (Promega). The pACD4K-C vector contains a T7 promoter and ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with the supernatant (total protein amount of 20-30 μg) supplemented with 6.4 mM MgCl2 and 0.5 U/μl Recombinant RNase Inhibitor (Promega) in total volume of 30 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... modified hypotonic lysis buffer with a 10-fold higher concentration of Cycloheximide and Recombinant RNasin Ribonuclease Inhibitor (Promega) was used instead of RNase OUT ...
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bioRxiv - Cell Biology 2020Quote: ... and immersed in 100 µL of growth medium and 20 µL of CellTiter 96 AQueousOne Solution Reagent (Promega Co.). After another incubation for 3 h at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2022Quote: ... and the cells were incubated in the growth medium supplemented with 5µM of cell-permeable Renilla luciferase substrate EnduRen (Promega) at 37C directly in an ID3 multiwell plate reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2019Quote: Cell growth was assessed using Proliferation Assay™ Kit in accordance with the manufacturer’s instructions (Promega, Madison, WI, USA) [15 ...