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Citations for Promega :
701 - 750 of 1462 citations for Recombinant Human FGF23 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega) and verified by Western blotting with the anti-HaloTag antibody (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were revealed using an ECL Western Blotting Substrate (Promega, USA).
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bioRxiv - Bioengineering 2023Quote: ... Proteins were cleaved via trypsin and Lys-C endoproteinase (Promega, Madison, WI), at a ratio of 1:50 enzyme to protein overnight (12-16 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50 μg total protein was then digested with 1 μg trypsin (Promega) overnight at 30 °C in the presence of 0.5% SLS ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were revealed using an ECL western blotting substrate (Promega, USA) and images were digitized using a Fusion FX (Vilber Lourmat ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described71 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were digested overnight in 25 mM ammonium bicarbonate with trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Biophysics 2023Quote: ... The vectors expressing other HaloTag-conjugated proteins were: H2B (pFC15A backbone, Promega) 15 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were digested overnight with 1ul of 0.1μg/ul LysC (Promega) and 2ul of 0.4μg/ul trypsin (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were digested with Trypsin/LysC mix (Promega, Wisconsin, WI, USA) at a 1:50 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then in-gel digested using modified trypsin (sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Microbiology 2023Quote: ... and so proteins were instead digested using sequencing grade gluC (Promega Corporation) added to the solution in a 1:20 mass ratio ...
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bioRxiv - Biochemistry 2023Quote: GsaB-modified GsaA precursor protein was digested with trypsin (Promega, sequencing-grade) at 37 °C for 20 min. ...
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bioRxiv - Immunology 2023Quote: ... Protein was digested with either trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a mass ratio of 1:30 overnight at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Biochemistry 2023Quote: ... A whole-cell protein extract of Saccharomyces cerevisiae (Promega, V7341, lot 434786) was deployed ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested in-gel using trypsin (600 ng/band, Gold, Promega), as previously described [29] ...
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bioRxiv - Plant Biology 2023Quote: ... The TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260) was used to generate Halo-tagged proteins starting with pIX-HALO plasmids as described previously (Mukherjee et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The proteins were subsequently digested overnight with molecular grade trypsin enzyme (Promega) using a ratio of 1:50µg (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Biochemistry 2023Quote: ... Treated proteins were then digested with sequencing grade modified trypsin (Promega, V5111) and incubated overnight (37 °C ...
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bioRxiv - Plant Biology 2023Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... proteins isolated by gel electrophoresis were digested with trypsin (Promega, Wisconsin, USA) in 100 mM NH4HCO3 (pH 8.0) ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified proteins were digested with sequencing-grade trypsin (Promega, Madison, Wisconsin, USA) and separated by nanoAcquity UPLC (Waters ...
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bioRxiv - Developmental Biology 2024Quote: ... The protein-bead complexes were digested with a mixture of trypsin:LysC (Promega) at a 1:20 ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... subjected to protein precipitation using Chloroform/Methanol and digested with trypsin (Promega). Desalted and acidified peptides (0.1% TFA ...
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bioRxiv - Cell Biology 2024Quote: ... before proteolytic digestion with LysC (enzyme to protein ratio 1:100, Promega) for 3 h and with trypsin (1:25 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were on-bead digested using 500 ng Sequencing Grade Trypsin (Promega). The digestion was carried out at 37°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
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bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
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bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
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bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Cell Biology 2020Quote: ... The protein was digested for overnight with 5 ng/µL trypsin (Promega Corporation) in 50 mM ammonium bicarbonate at 37 °C ...