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Citations for Promega :
1 - 50 of 5275 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Molecular Biology 2019Quote: ... 10.0 ng/ml recombinant human FGF (Promega) and 1.0 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... pneumoniae D39 or the isogenic mutants towards human epithelial cells was accessed using a CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Epithelial cell proliferation was determined following the MTT procedure (Promega). Migration and invasion were assessed in culture using Transwells (Cultrex 24-well Cell Migration Assay plates ...
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bioRxiv - Biophysics 2021Quote: ... and 5 ng/ml recombinant human fibroblast growth factor (G5071, Promega).
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Biophysics 2023Quote: Transfected cells were stained with JF-549 tagged HaloTag ligand (Promega) and Vybrant DiO staining solution (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: Stable COMT cell lines were transfected with Myc-tagged ubiquitin using FugeneHD (Promega) following the manufacturer’s instructions using 4 μg plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were transiently transfected with tagged-target proteins using Fugene® HD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... HA-tagged DDX1and HA-tagged TRIF were constructed by inserting indicated sequences into pCMV-HA (Promega), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we implemented a cell-free assay with purified recombinant luciferase (Promega) and luciferin (BrightGlo Luciferase Assay System ...
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bioRxiv - Biochemistry 2021Quote: ... recombinant bacmids were transfected into Sf21 cells using Fugene6 (Promega E2693). Supernatant containing viruses (V0 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from isolated epithelial cells or tissue using the ReliaPrep RNA Tissue Miniprep System (Promega, USA) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: Nine DIV hippocampal slice cultures were randomly assigned to treatment groups with the following drugs dissolved in serum-free medium: recombinant human BDNF (250 ng/mL, Promega); BDNF + K-252a (200 nM ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant (rTdT) enzyme (Promega). Cells were then incubated at 37 °C for 60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Systems Biology 2020Quote: The Human cell lysate was obtained commercially (Promega) and the yeast digest was prepared as previously described23 ...
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bioRxiv - Biochemistry 2022Quote: Human cancer cell line digest standards K562 (Promega) and diluted to 100 microgram/mL in 50mM TEAB and labeled with TMTPro reagents according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Neuroscience 2020Quote: ... 1U/uL recombinant RNAsin(Promega), 10mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNasin Ribonuclease inhibitor (Promega), and SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and recombinant RNasin inhibitor (Promega). The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and ribonuclease (RNasin Recombinant, Promega) at concentrations of 1:1000 (sucrose buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...