Labshake search
Citations for Promega :
351 - 400 of 2267 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
-
bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 1 hour at 37 °C with RNasin Plus (N2611, Promega). The RNA was extracted with Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
-
bioRxiv - Cell Biology 2021Quote: ... They were then digested with mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
-
bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
-
bioRxiv - Microbiology 2023Quote: ... stored at −80 °C and analysed using Dual-luciferase substrate (Promega) in a FLUOstar Optima luminometer (BMG labTech) ...
-
bioRxiv - Pathology 2023Quote: ... MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St ...
-
bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
-
bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were then proteolyzed with Trypsin/Lys-C Mix (Promega, V5071) at a 25:1 protein ...
-
bioRxiv - Immunology 2022Quote: ... and the RNA was precipitated with an equivalent volume of isopropanol and incubated at 55°C for 10 minutes before being resuspended in 50 μL of RNase-free water (Promega, Madison, WI, USA). We determined the concentrations of RNA using a Smart-Spec plus spectrophotometer (Bio-Rad) ...
-
bioRxiv - Microbiology 2020Quote: ... collected by centrifugation (17,000 g for 10 min at 4°C) and solubilized in 20 μl 50 mM TEAB containing 0.2 % ProteaseMAXTM Surfactant (Promega UK Ltd, Cat. # V2071) for 1-2 h with vortex and occasional sonication ...
-
bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μg of the isolated recombinant bacmid and 5 μL Fugene HD transfection reagent (Promega) were diluted in 150 μL Sf-900™ III SFM ...
-
bioRxiv - Cell Biology 2020Quote: ... Halotag fusion proteins were labelled with Janelia Fluor 646 Halotag Ligand (Promega) for live cell imaging ...
-
bioRxiv - Developmental Biology 2019Quote: SBROS cells were stained with the HaloTag TMR Ligand (Promega, Cat#G8251) and the SNAP-SiR647 dye (NEB ...
-
bioRxiv - Cell Biology 2021Quote: ... containing 100 nM bafilomycin A1 and 37.5 nM TMRDirect Halo Ligand (Promega). After 2 hours in EBSS ...
-
bioRxiv - Cell Biology 2019Quote: ... Halo-tag expressing cells were labelled with Halo-TMR ligand (Promega - G8251) following the manufacturers instructions for live cell labelling prior to fixation and permeabilization.
-
bioRxiv - Systems Biology 2019Quote: ... Cell media was supplemented with HaloTag® TMRDirect™ Ligand (Promega Corporation) 16-24 h after seeding to a final concentration of 20 nM and cells were incubated for an additional 16-24 h ...
-
bioRxiv - Microbiology 2021Quote: ... live cells were treated with cell-permeant Halotag TMR ligand (Promega, G8251) before paraformaldehyde fixation ...
-
bioRxiv - Cell Biology 2022Quote: ... Then they are incubated with an Alexa Fluor488 Halotag® ligand (Promega) (400 nM per 1 ml containing approximately 1.5-2 million cells ...
-
bioRxiv - Cell Biology 2022Quote: The HaloTag-holocomplexes were labeled with HaloTag Alexa Fluor 488 Ligand (Promega) at room temperature for 30 min ...
-
bioRxiv - Microbiology 2022Quote: ... 1mL of the culture was stained with 50nM HaloTag TMR Ligand (Promega) for 30 min at 30 °C ...
-
bioRxiv - Microbiology 2022Quote: ... 1mL of the culture was stained with 250nM HaloTag TMR Ligand (Promega) for 30 min at 30 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were stained with Janelia Fluor (JF) 549 conjugated Halotag ligands (Promega) at 200 nM concentration in 2% FBS/PBS at 37 °C for 15 min ...
-
bioRxiv - Cell Biology 2023Quote: Cells were incubated with 100 nM TMR-conjugated HaloTag ligand (G8251, Promega) for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... and incubated in 2 nM JF549-Halo-ligand (Cat. No. GA1110; Promega) for 15 minutes at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... HaloTag® Succinimidyl Ester (O2) Ligand was purchased from Promega (Cat #1691), dissolved in DMSO ...
-
bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
-
bioRxiv - Biophysics 2023Quote: ... or Janelia Fluor® 635 HaloTag Ligand (MNRs) (Cat. No. CS315103, Promega) or Janelia Fluor® 646-BAPTA-3’AM-HaloTag ligand (requested from Lavis lab ...
-
bioRxiv - Pathology 2020Quote: ... the 60°C annealing/extension temperature recommended by the mastermix manufacturer (Promega) produced more robust amplification (data not shown).
-
bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
-
bioRxiv - Biochemistry 2021Quote: ... and finally digested overnight at 37 °C with 375 ng Trypsin (Promega). The peptides were extracted from the gel twice using 100 μl of 50 % acetonitrile and 0.25 % TFA buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
-
bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteolytic digestion was carried out with a trypsin/Lys-C mixture (Promega) incubated for 18 h at 37°C at a protein to protease ratio of 25:1 ...
-
bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: ... the plasmid was re-ligated overnight at 16°C (ligase from Promega) and transform in Stbl4 bacteria ...
-
bioRxiv - Microbiology 2020Quote: ... The samples were treated with trypsin/Lys-C protease mix (Promega, V5072) at a protease:protein ratio of 1:25 for 16 h at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... For digestion Typsin/Lys-C mass spectrometry grade proteases mixture from Promega Corporation was used ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
-
bioRxiv - Biophysics 2020Quote: ... Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
-
bioRxiv - Developmental Biology 2022Quote: ... which were digested with 1:50 Arg-C protease (Promega, cat. #V1881): histone protein at 37°C for 12-16 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Bands digested overnigt at 37 °C with 800 ng of trypsin (Promega) and/or chymotrypsin (Promega ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight with Sequencing Grade Modified Trypsin/Lys-C (Promega) at 370C ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... in-gel digestion was performed using 0.10 µg trypsin/Lys-C (Promega) overnight in 25 mM NH4HCO3 at 30 °C ...