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Citations for Promega :
401 - 450 of 616 citations for Recombinant Human Apolipoprotein A I Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were prepared from some positive clones using the PureYield Plasmid Miniprep System (Cat #A1222, Promega, Madison, USA). The sequencing of the cloned insert was carried out according to Sanger et al ...
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bioRxiv - Biochemistry 2019Quote: The 5’UTRs of each transcript variant were transcribed and purified in vitro as previously described46 from linearized recombinant psiCHECK2 vector (Promega) that contained one of the three UTR sequences downstream of a T7 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Cell Biology 2022Quote: ... 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 μl of collection buffer [1.0U/μL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Molecular Biology 2023Quote: ... The complexes were washed with RIPA buffer and the samples were treated with DNAse I (Promega) for 30 minutes at 37°C ...
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bioRxiv - Genomics 2024Quote: ... (i) quantification of DNA samples of multiple sexed green toads (QuantiFluor® dsDNA System kit, Promega), (ii ...
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bioRxiv - Cancer Biology 2024Quote: ... total RNA was isolated and DNAse I-treated using Maxwell® RSC simplyRNA Tissue kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and pooled human male DNA (G3041, Promega and 4312660, Life Technologies) were used to generate calibration curves and serve as reaction controls ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Cell Biology 2021Quote: ... Human normal genomic DNA was obtained from Promega (Madison, WI, USA) and the values are averages of three replicates ...
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bioRxiv - Cancer Biology 2020Quote: ... in a background of 130ng of normal human genomic DNA (Promega) was used as a positive control ...
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bioRxiv - Cell Biology 2019Quote: ... and human samples were performed using GoTaq green master mix (Promega) and the following amplification conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... 830bp or 800bp human STIM1 promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
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bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Biophysics 2023Quote: ... The initial recombinant baculovirus was generated by transfecting adherent Sf9 cells with the bacmid DNA using the FuGENE HD reagent (Promega, USA) and used to infect suspension cells for virus amplification ...
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bioRxiv - Developmental Biology 2024Quote: ... The katna1 digoxigenin-labelled sense and antisense riboprobes were synthetized from the linearised recombinant TOPO® TA cloning vector using T7 and T3 RNA Polymerase (Promega) according to the supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... were extracted from 30 eggs and treated with DNase I using ReliaPrep RNA Tissue Miniprep System (Promega). cDNAs were synthesised from the purified RNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Biochemistry 2020Quote: ... For i) Reduced and carbamidomethylated nMPO was digested in technical triplicates using sequencing-grade porcine trypsin (Promega), ii ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB], 0.4 μl RNasin [Promega] ...
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bioRxiv - Developmental Biology 2021Quote: ... DNase I treatment and cDNA synthesis were preformed using the RQ1 RNASE-FREE DNASE 1* (Promega®) and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific®) ...
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bioRxiv - Microbiology 2023Quote: ... 1–2 μg of each RNA was treated with RNase-free Dnase I (Promega, Madison WI, USA) and reverse transcribed using the PrimeScript RT reagent Kit (TaKaRa) ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were T-cloned using the pGEM®-T Easy Vector System I (cat A1360, Promega) and plated on X-gal/IPTG plates ...
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bioRxiv - Immunology 2023Quote: The IL2 URR was cloned into Xho I and Hind III sites of PGL4 Luc vector (Promega) and then ∼500 bp individual sequence representing IL2-IL21 intergenic CNS at −46 ...
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bioRxiv - Molecular Biology 2023Quote: ... HDAC activity of three technical replicates was measured using the HDAC-Glo I/II Assay (Promega, #G6420) following the manufacturer’s protocol on-bead with an Envision plate reader (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cloned into pGEM-T Easy vector using pGEM-T Easy Vector System I (Promega; CAT A1360). Vectors were transformed into Escherichia coli using the heat-shock method ...
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bioRxiv - Genomics 2024Quote: ... We amplified the Cytochrome c oxidase subunit I (COI) for individual abdomen DNA using the GoTaq (Promega) protocol ...
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bioRxiv - Systems Biology 2020Quote: ... albicans as well as of a commercially available human protein digest (Promega), which was exclusively used in this experiment ...
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bioRxiv - Biochemistry 2023Quote: MS-Compatible Human Protein Extract Digest (K562) was purchased from Promega (V6951), MassPREP E ...
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bioRxiv - Cell Biology 2023Quote: ... human BLTP2/KIAA0100 ORF was amplified from pFN21A-Halo-KIAA0100 (FHC00016, Promega). An unstructured linker (sequence ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The recombinant plasmid was digested with Sac1 and Nde1 restriction enzyme and sub-cloned in pGL3 Basic luciferase vector (Promega, Madison, USA).
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Biochemistry 2019Quote: ... DMEM media was aspirated from the cells and 500 µL of hypotonic lysis buffer (50 mM Tris.HCl pH 7.4, 25 units/mL DNase I (Promega), 0.2 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Final enrichment was achieved with a nuclease digestion using DNase I and S1 nucleases (Promega Corporation, Madison, WI). Enriched dsRNA was shipped overnight on dry ice to Quick Biology (Pasadena ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected by using SYBR Green I (GO-Taq PCR Master mix, Promega, Walldorf, Germany; A6002). Suitable reference genes were identified by using geNorm [47] ...
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bioRxiv - Cancer Biology 2023Quote: ... and amplicons were detected using SYBR Green I (GO-Taq PCR Master mix, Cat: A6002; Promega, Walldorf, Germany). Sequences of the primers used in this study are listed in Supplementary Table S2 ...