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Citations for Promega :
1 - 50 of 4403 citations for Rat Protein SET SET ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Amplification was set up with the qPCR kit (Promega, Madison, WI) and carried out on an ABI7500 with an initial melting temperature of 95°C for 2 mins and 40 cycles of 95°C for 15 sec and 60°C for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... Set of each dNTPs (U1130) (Promega); formaldehyde (P2031) ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... EVEN or LacZ probe sets were solubilized in Trypsin Buffer (Promega) for digestion into small peptides ...
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bioRxiv - Cell Biology 2023Quote: ... qPCR reactions were set up with GoTaq® qPCR Master Mix (Promega) using diluted cDNA (5 ng/µl) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μl reactions were set up using GoTaq® Green Master Mix (Promega). Primers used to amplify zfccr5 ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR validation system was set up using GoTaq Green Master Mix (Promega) with the following components ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions were set using the RiboMAX™ Large Scale RNA Production System (Promega) and incubated at 37ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... qPCR reactions were set up with GoTaq qPCR Master Mix reagent system (Promega) and run on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... with the primers sets (BE3_PCR_F and BE3-PCR_R) using Pfu DNA polymerase (Promega, M7741). Amplified fragments were digested with XhoI and NotI and cloned into pCAGIG plasmid (Addgene ...
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bioRxiv - Plant Biology 2022Quote: ... DNA digestion was performed using the RQ1 RNase-free DNase set (Cat No.: M6101, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... SYBR Green-based RT-qPCR reactions were set up using GoTaq qPCR Master Mix (Promega) and 20 ng of input cDNA per each 10-μL reaction ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR reactions were set up in triplicates with GoTaq® qPCR Master Mix (Promega, United States) and run in a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... SYBR Green-based RT-qPCR reactions were set up using the GoTaq qPCR Master Mix (Promega) and 20 ng input cDNA per each 10-μL reaction ...
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bioRxiv - Molecular Biology 2019Quote: ... and set at room temperature for 30 min prior to reading with a GloMax luminometer (Promega). Integration time was set at 1 second 54,55.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... PCR reactions (25 µL) were set up with GoTaq Green Master Mix (Promega, Madison, WI, USA), 200 nM of forward and reverse primers (Table S40) ...
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bioRxiv - Biochemistry 2020Quote: Transcription assays were set up using the HeLa Scribe Nuclear Extract in vitro Transcription system (Promega, catalog # E3110) following the manufacture’s protocol with modifications ...
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bioRxiv - Plant Biology 2020Quote: The qRT-PCRs/qPCR reactions were set up using the GoTaq® qPCR Mastermix (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions in a total volume of 15 μl ...
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bioRxiv - Genomics 2019Quote: ... We then set up quantitative PCR reactions with 5 µL GoTaq qPCR Master Mix (Promega, Madison, WI, USA), 1 µL each forward and reverse primer at 5 µM ...
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bioRxiv - Neuroscience 2022Quote: ... Once all reactions had been set up the plate was moved to a GloMax® Explorer plate reader (Promega) and incubated for 40 min at 25 °C before reading for luminescence ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were set up as follows: 6.25 µl of GoTaq® qPCR Master Mix (Promega, Madison, WI, USA), 4.25 µl of nuclease-free water ...
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bioRxiv - Cell Biology 2023Quote: ... were PCR-amplified from genomic DNA extracted from EndoC-βH3 cells with primer sets (Table S5) designed by Primer3-based software and cloned into pGL4.23[luc/minP] vector (Promega) between KpnI and XhoI restriction enzyme sites ...
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bioRxiv - Microbiology 2021Quote: ... spxB gene region containing the ORF (amplified from R6 genome using primer set 1-2) was cloned into pGEM-T Easy vector (Promega). spxB ORF was disrupted by cloning a kanamycin resistance cassette (amplified from pIBD38 ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... In-solution digestion was performed overnight on a shaker set at 37 °C using sequencing grade trypsin (Promega, Madison, WI) at ∼1:25 w/w enzyme/protein ratio ...
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bioRxiv - Biophysics 2019Quote: ... The N-terminal Halo-tagged vector segment was cloned by using the primer sets: 5’-GAGTAACTAGCATAACCCCTTGGC-3’ and 5’-CACTAGCCATGTTATCGCTCTGAAAGTACAGATC-3’ with the pHTN HaloTag® CMV-neo Vector (Promega) as template ...
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bioRxiv - Cell Biology 2021Quote: Realtime-Glo MT Cell Viability assay: The assay was set up as a continuous read format according to the manufacturer’s instructions (Promega, Madison, Wl) in a 96 well format using 5,000 cells/well in a 100ul volume ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 250 µL of BMB was added to each well and the optical density of the samples was read using a Glomax plate reader set at 525 nm (Promega, Madison, USA). The amount of GAG in each sample is expressed as a percentage of the dry weight of the digested ECM.
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bioRxiv - Microbiology 2023Quote: ... we confirmed that each primer set amplified single bands in a ligation- dependent manner by GoTaq Hot Start Green Master Mix (Promega, Madison, WI) (Figure S6) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a set of cells seeded in parallel were lysed and measured using 40 μL CellTiter-Glo luminescent cell viability reagent (Promega product code G7570) per well as per the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2019Quote: ... purified amplicons were inserted into plasmids and ligation reactions (10 μ1) were set up as per manufacturer’s instructions (Promega pGEM-T Easy vector system). After ligation ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
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bioRxiv - Microbiology 2024Quote: ... Amplifications were set up in a total volume of 20 μL containing: 10 μL GoTaq® Green Master Mix (Promega Corporation, Madison, Wisconsin, USA), 1 μL of each 10 μM primer ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...