Labshake search
Citations for Promega :
351 - 400 of 4879 citations for Rat Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
-
bioRxiv - Physiology 2023Quote: ... and digested (0.4 ug of Lys-C/Trypsin mix, Promega). After 4 hours of digestion in the urea buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
-
bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
-
bioRxiv - Microbiology 2020Quote: ... faginata MAT1-1 isolate using a CTAB-chloroform DNA extraction method (van Diepen et al. 2017) and a MAT1-2 isolate using a Wizard® kit (Promega, Madison, WI, USA) and suspended in 75 µl Tris-EDTA (TE ...
-
bioRxiv - Neuroscience 2019Quote: ... The U6 reporter construct was assembled by ligating a 663bp rat U6 sequence into the HindIII and NheI sites of the pGL4.23 vector (Promega) upsteam of the minimal promoter in this vector ...
-
bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
-
bioRxiv - Genetics 2019Quote: ... and digestion were performed by dithiothreitol (DTT, 10mM, 30min at room temperature), iodoacetamide (IAA, 10mM, dark for 30min at 37°C) and trypsin (MS Grade, Promega, 12.5ng/ul, 37°C overnight) sequentially before analyzing by Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer ...
-
bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2 µl of random primers (Promega) were added after which the mixture was incubated at 65 °C for 5 min ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 2 μg of Trypsin (Promega, V5111) was added to each sample and they were incubated shaking at 37°C overnight (o/n) ...
-
bioRxiv - Cancer Biology 2020Quote: ... 0.25 µl of 2% digitonin (Promega) and 0.5 µl of 10% Tween-20 ...
-
bioRxiv - Neuroscience 2021Quote: ... 2 U GoTaq Flexi Polymerase (Promega) and 0.22 µg TaqStart Antibody (Clontech) ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 μL Superasin RNase inhibitor (Promega), and 8 μL PEG8000 (supplied with ligase) ...
-
bioRxiv - Molecular Biology 2020Quote: The commercial psiCHECK™-2 (Promega) vector was modified by deleting the Rluc poly(A ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 U μL-1 RNasin (Promega), 1 mM ATP/GTP/CTP ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 U/μl RNaseIn Plus (Promega) and 10 U/μl SuperScript IV Reverse Transcriptase enzyme while heated ...
-
bioRxiv - Neuroscience 2022Quote: ... The psiCHECK-2 vector (Promega, C8021) was used to build the dual luciferase reporters with Kcnj10 UTRs ...
-
bioRxiv - Systems Biology 2022Quote: ... 2 µl trypsin (Promega Trypsin Gold) was added pre-diluted in ultra-pure water to 2 ng/µl and incubated overnight at 37°C in the thermal cycler ...
-
bioRxiv - Cell Biology 2023Quote: ... The psiCHECK-2 vector (Promega, C8021) and the psiCHECK-RL-Ifnb1 3’ UTR vector were described before (Zhang et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 20 ng/well of psiCHECK-2 plasmid (psiCHECK-2 Vector (V0) (Promega, C8021) or let-7a-mi6 targeting six regions of the 3ill UTR ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 1h at 37°C in presence of random hexamers (Promega). qPCR primers (supplement Table 1 ...
-
bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were purified using C18 spin columns (Pierce ...
-
bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega) using S-Trap (Protifi ...
-
bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
-
bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
-
bioRxiv - Cell Biology 2021Quote: ... digested overnight with Trypsin/Lys-C (1:20 w/w, Promega), desalted using C18 Hypersep Spin Column (Thermo) ...
-
bioRxiv - Cell Biology 2020Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were cleaned up using C18 spin columns (Pierce ...
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... pH 7 and digested with 500 ng recombinant Lys-C (Promega) at RT while shaking at 1,400 rpm ...
-
bioRxiv - Immunology 2022Quote: ... dehydrated and proteolytically cleaved overnight at 37 °C with trypsin (Promega). The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore ...
-
bioRxiv - Biophysics 2021Quote: ... The LgBiT fragment amplified from pBiT1.1-C [TK/LgBiT] Vector (Promega) using KOD One PCR Master Mix (TOYOBO ...
-
bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 1 hour at 37 °C with RNasin Plus (N2611, Promega). The RNA was extracted with Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1 ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
-
bioRxiv - Cell Biology 2021Quote: ... They were then digested with mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
-
bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
-
bioRxiv - Microbiology 2023Quote: ... stored at −80 °C and analysed using Dual-luciferase substrate (Promega) in a FLUOstar Optima luminometer (BMG labTech) ...
-
bioRxiv - Pathology 2023Quote: ... MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St ...
-
bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... 5mM DTT containing 10 ng/μL trypsin/Lys-C mixture (Promega), and incubated overnight at 25 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
-
bioRxiv - Cell Biology 2023Quote: ... Beads were then proteolyzed with Trypsin/Lys-C Mix (Promega, V5071) at a 25:1 protein ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and the orthologous 208 bp blind mole rat sequence (nanGal1 assembly; KL203377:255659-255866) into a luciferase pGL4.23[luc2/minP] vector (Promega, US). We cloned the insert in the same orientation with respect to the minP promoter and luciferase gene as the CNE and the Tdrd7 promoter are oriented in the genome ...
-
bioRxiv - Biochemistry 2022Quote: ... The half site ARE reporter is derived from the minimal rat probasin sequence (Zhang et al., 2000) and was cloned into pGL3 via KpnI and NcoI restriction sites (Promega). pRL-TK (Promega ...
-
bioRxiv - Neuroscience 2021Quote: The Cacnb2 3’UTR (Ensemble ID: ENSG00000165995) was amplified from a rat brain cDNA library and cloned into the pmirGLO dual-luciferase expression vector (Promega) using the PfuPlus! DNA Polymerase (Roboklon) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) (Promega, Cat. No. G109C) cell viability assay was performed ...
-
bioRxiv - Cell Biology 2022Quote: ... β-arrestin 2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin 2; Promega, plasmid no. CS1603B118) was chosen as it has previously been used successfully with other class B GPCRs ...