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Citations for Promega :
151 - 200 of 6930 citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Amplification of genes utilized GoTaq qPCR Master Mix (Promega) as per the manufacturer’s protocol on the LightCycler® II 480 system machine (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Immunology 2019Quote: ... Proteins were digested with sequencing grade trypsin (1:100; Promega, USA) overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000 ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Plant Biology 2021Quote: ... and tryptic protein digestion (1:100 enzyme:protein, V5111, Promega, Madison, USA) was performed using Microcon-30 kDa filter units (Millipore ...
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bioRxiv - Biophysics 2020Quote: ... The proteins were digested overnight with trypsin (1:50) (Promega, USA) in 1% (w/v) ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested by addition of 1 µg of Trypsin (Promega) and incubated overnight at 37 °C at 600 rpm on a thermoshaker ...
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bioRxiv - Biochemistry 2023Quote: ... then digested with lysC/trypsin mixture (1: 50, enzyme: protein, Promega) at 37℃ overnight ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37°C ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Biophysics 2021Quote: Some protein kinase assays were performed using the ADP-Glo™ assay kit (Promega), which measures the generation of ADP in a kinase reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Neuroscience 2023Quote: LDH release from macrophages treated with Aβ profect complexes was measured using the CytoTox-ONE Homogeneous Membrane Integrity Assay (cat no: G7891, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Pyroptotic cell death was measured by assessing LDH-release in the cell culture supernatant of human and murine macrophages using a CytoTox 96 Non-radioactive Cytotoxic Assay (Promega) following the manufacturer’s recommended procedures.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Cell Biology 2023Quote: HeLa FlpIn were co-transfected with pCDNA5 constructs with gene of interest and construct of pOG44 recombinase in a 1:9 ratio with Fugene HD (Promega, E2311) under the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: His-P150-CC1 (Courtois et al., 2012) was expressed in and purified from BL21(DE3)pLysS competent cells (Promega) as previously described (Courtois et al. ...
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bioRxiv - Physiology 2020Quote: ... Transfection efficiency was measured by normalization to Renilla luciferase activity expressed from a co-transfected pTK-RL vector (Promega).
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Molecular Biology 2019Quote: ... the Renilla and Firefly luciferase genes in psiCheck2 plasmid (Promega) were first replaced by constructs with synonymous mutations in putative m6A sites (obtained as IDT gBlocks) ...
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bioRxiv - Plant Biology 2020Quote: The OsSalT gene was cloned into pGEMT-Easy vector (Promega) followed by sub-cloning into the EcoRI and BamHI RE sites of pEGAD vector under control of CaMV35S promoter ...
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bioRxiv - Cell Biology 2021Quote: ... The Halo-Tag or NanoLuc genes were acquired from Promega and were genetically fused to the respective N- or C-termini.
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bioRxiv - Microbiology 2020Quote: ... The human codon-optimized NanoLuc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Developmental Biology 2020Quote: ... To localize the regions of gene expression NBT/BCIP (Promega) in alkaline phosphatase buffer (See Table S3 for composition ...
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bioRxiv - Biochemistry 2020Quote: ... The luciferase reporter gene synthesized into a pGL3basic vector (Promega), and sub-cloned in EcoRI and XhoI sites of the CEN/ARS ...
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bioRxiv - Immunology 2020Quote: ... The human codon-optimized nanoluc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Synthetic Biology 2023Quote: Gene constructs were cloned into the pTNT™ vector (Promega). BL21(DE3 ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50 μg total protein was then digested with 1 μg trypsin (Promega) overnight at 30 °C in the presence of 0.5% SLS ...
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bioRxiv - Neuroscience 2024Quote: ... and digested with trypsin (1:50 enzyme: protein ratio; Promega, United States) at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... before proteolytic digestion with LysC (enzyme to protein ratio 1:100, Promega) for 3 h and with trypsin (1:25 ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako) and 1 μg trypsin (Promega) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2020Quote: Degenerate primers specific to each gene were designed using Primer3plus software [https://primer3plus.com/] for all 17 identified CKX genes following the guidelines for primer design provided by the PCR amplification kit manufacturer (Promega Corporation, USA; Table S2). PCR amplification of the identified CKX GFMs was performed using the reaction kits selected based on the gene size ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... rat anti-Fat (kind gift from Helen McNeill) was used at 1:500 and mouse anti-β-galactosidase (Z3781, Promega; RRID:AB_430877) was used at 1:500 ...
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bioRxiv - Immunology 2020Quote: ... −75 to −1 in relative to the transcription start site (TSS) of Il13 gene) and P150 (−150 to −1) were cloned into pGL3-basic luciferase vector (Promega, Madison, WI) in BglII and HindIII cloning sites ...
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bioRxiv - Immunology 2021Quote: ... Media was replaced with 100ul of VIM and viability of macrophages was then assessed by the addition of 100ul of Cell Titer Glo (Promega G7573) per well which was read for luminescence (Biotek ...
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bioRxiv - Microbiology 2020Quote: ... GoTaq 1-Step RT-qPCR kit (Promega) was used at a final volume of 20 μl with ...
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bioRxiv - Plant Biology 2021Quote: ... Protein preparation and luciferase– substrate interaction were conducted with a Luciferase Assay Report Kit (Promega). Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash) ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Microbiology 2020Quote: ... Cell fusion activity was quantitatively determined after 24 h by measuring firefly luciferase activity expressed by pT7EMCVLuc and was standardized with Rluc activity expressed by pRL-CMV by using the Dual-Glo luciferase assay (Promega) (27 ...
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bioRxiv - Microbiology 2019Quote: ... vortexed for 5 s and then immediately measured for luminescence (expressed as relative light units [RLU]) using a GloMax 20/20 Luminometer (Promega). All strains were tested in quadruplicate from three independent cultures.