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Citations for Promega :
651 - 700 of 4410 citations for Rat High Mobility Group Protein B1 HMGB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Protein pellets were resuspended in 8 M urea buffer combined with protease inhibitor (Promega) and purified using the chloroform-methanol precipitation method (Wessel & Flügge ...
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bioRxiv - Biophysics 2022Quote: ... The purified Kif5B protein was incubated with 10 µM HaloTag PEG-Biotin ligand (Promega) for 30 min on ice to produce a final construct of Kif5B homodimer with two C-terminal biotin tags ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Cancer Biology 2019Quote: Proteins were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation methods[13] ...
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bioRxiv - Immunology 2020Quote: ... IgG was purified from culture supernatant using Magne Protein A beads (Promega, Madison, WI) and the elution buffer exchanged with PBS using Amicon Ultra centrifugal filters (Millipore-Sigma ...
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bioRxiv - Immunology 2021Quote: Proteins were in-gel digested with sequencing grade modified trypsin (Promega GmbH, Walldorf, Germany) similar to the procedure described by Pandey et al ...
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bioRxiv - Neuroscience 2020Quote: ... samples containing 100 μg total protein were mixed with 100 μl Luciferase Substrate (Promega). The kinetics of the luminescence was recorded for 10 min using a TriStar2 S LB 942 Plate Reader (Berthold Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega) and 2 μg LysC (Promega) ...
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bioRxiv - Cell Biology 2021Quote: Proteins were synthesized using TNT® SP6 Quick Coupled Transcription/Translation system (Promega L2080) using the standard reaction mix (rabbit reticulocyte lysate plus amino acids ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were digested for 18 hours at 37 °C with trypsin/LysC mix (Promega) at a protein-to-protease ratio of 25:1 ...
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bioRxiv - Systems Biology 2020Quote: ... Proteins were digested for 18-24 hours on beads with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio for frozen tumors and 2 μg trypsin per 10 μm section of FFPE ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... The protein extract was digested with trypsin (Catalog No. V5280, Promega, Madison, WI, USA) (trypsin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Microbiology 2020Quote: ... Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501) using a Spectramax L luminometer with reagent injectors in technical triplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (200 µg) were digested overnight with Lys-C (Wako Chemicals) and trypsin (Promega) using the filter-aided sample preparation protocol29 ...
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bioRxiv - Neuroscience 2020Quote: ... Bound proteins were digested on-resin with trypsin (Trypsin Gold, Mass spectrometry grade, Promega) at 37 °C overnight in 25 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2019Quote: ... The translated protein was analyzed using a Transcend Non-Radioactive Translation Detection System (Promega).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... alongside 5 µl of broad molecular weight protein marker (Broad Range Molecular Marker, Promega), was loaded onto the gel and run at 200 volts for 55 minutes using a Mini-PROTEAN Electrophoresis System (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... Proteins were in-gel digested using 0.6 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate overnight at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were further digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Plant Biology 2020Quote: ... and used for protein digestion with sequencing-grade modified trypsin (Promega, Madison, WI, USA). Resulting peptides were purified using Sep-Pak Vac tC18 100 mg cartridge (Waters ...
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bioRxiv - Immunology 2020Quote: ... The proteins were digested with Trypsin Gold overnight at 37°C (Promega, Madison, WI). The resulting peptides were de-salted using C18 SPE cartridges (Waters ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...
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bioRxiv - Synthetic Biology 2020Quote: ... Proteins were digested with two separate and sequential aliquots of sequencing grade trypsin (Promega) of 1 μg ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Physiology 2020Quote: The protein solution samples were hydrolyzed into peptide segments by trypsin (V9012, Promega (Beijing) Biotech Co. ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were transiently transfected with tagged-target proteins using Fugene® HD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... 50 μl of 0.5M TEAB and trypsin (Promega, enzyme: protein ratio of 1:50) were added to the membrane ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested ingel using modified trypsin (sequencing grade, Promega, Charbonnières les Bains/France) as previously described (50).
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bioRxiv - Microbiology 2021Quote: ... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Protein digestion was accomplished by overnight incubation at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Biophysics 2022Quote: ... The protein was digested at 37 °C overnight by adding sequencing-grade trypsin (Promega) at a 24:1 substrate-to-enzyme ratio ...
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bioRxiv - Biochemistry 2022Quote: ... ⑤Resuspended the denatured proteins with 25 mmol/L NH4HCO3 and digested with trypsin (Promega, Fitchburg ...
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bioRxiv - Plant Biology 2022Quote: ... PsiYAB11 protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega). The protein-bound beads were incubated with 50 ng of adaptor-ligated gDNA fragments on a rotator for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Neuroscience 2022Quote: ... Protein enzymatic cleavage was carried out with trypsin (1:20, w/w; Promega, V5280) at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were precipitated with acetone and trypsin-digested using sequencing grade-modified trypsin (Promega). The resulting peptide mixture was purified by STAGE-TIP method using a C18 resin disk (3M Empore ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins eluted at pH 9.0 were further treated with 5 U of CIAP (Promega) and the buffer provided by the manufacturer (a negative control was prepared without phosphatase) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The proteins were digested with sequencing-grade modified trypsin (Promega Corporation, Madison, WI, USA). The peptides were analyzed using a nano LC–MS system (UltiMateTM 3000 RSLCnano and Orbitrap ExplorisTM 240 ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were digested with chymotrypsin (5 ng/μL) at 25ºC overnight (Trypsin gold, Promega). Digestion was stopped by addition of 5% formic acid and peptides extracted twice with 70% acetonitrile and 5% formic acid (10 min sonication) ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...