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Citations for Promega :
1 - 50 of 6682 citations for Rat Heat Shock 60 kDa Protein 1 Chaperonin HSP60 HSPD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The heat shock element (HSE) firefly luciferase reporter was purchased from Promega (Madison, WI) and has two copies of the HSE promoter driving the luc2P gene ...
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bioRxiv - Cell Biology 2023Quote: Heat shock-induced cytotoxicity was determined using the LDH-Glo cytotoxicity assay (Promega, catalog number: J2380) according to the manufacturer’s guidelines ...
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bioRxiv - Systems Biology 2020Quote: Cell viability after heat shock and during recovery was estimated with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cells were pelleted ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μL of each ligation reaction were used for heat-shock transformation of 50 μL of JM109 High-Efficiency Competent Cells (Promega, Madison, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... treated with rubidium chloride and transformed by thermal shock (Promega Protocols and Applications Guide). The cells inoculated in LB broth (USP Corp ...
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bioRxiv - Biophysics 2020Quote: ... The construct expresses rat GR fused with HaloTag protein (Promega, Madison, WI, USA) in the C-terminal domain under the CMVd1 promoter ...
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bioRxiv - Immunology 2023Quote: ... Insulin and NEFA levels were using a mouse insulin ELISA kit (Promega) and WAKO NEFA-C kit (WAKO ...
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bioRxiv - Microbiology 2022Quote: ... and SV40 late poly(A) signal was amplified from pNL1.1.CMV[Nluc/CMV] (19.1 kDa NanoLuc protein, Promega). The PCR product was blunt end ligated to pAD1 digested with EcoRV ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: HaloTag-GST (61 kDa) (Promega, G4491) was labelled with JF646 for 4 hours at 4 ° C on ice with 3x excess HaloTag ligand (buffer ...
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bioRxiv - Genetics 2021Quote: ... and Pierce 660nm Protein Assay kit (Promega) was used for quantification ...
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bioRxiv - Biochemistry 2022Quote: ... 60 μM amino acid mixture (Promega), 50 μM Spermidine ...
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bioRxiv - Molecular Biology 2023Quote: ... and 60 U T7 polymerase (Promega) were incubated at 37 °C for 2 h ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Cell lysates were pre-cleared of any non-specific streptavidin-binding proteins by incubating with 60 µL of MagneSphere paramagnetic particles (Promega, Madison, WI) for 30 min with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... A direct cAMP ELISA kit (Enzo) was used in endpoint experiments and the pGloSensor (−20F) luminescence assay (Promega) was used for kinetic cAMP measurements ...
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bioRxiv - Bioengineering 2019Quote: ... 60 μL 2x GoTaq Master Mix (Promega), 12 μL 10 μM forward primer (FP) ...
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bioRxiv - Cancer Biology 2022Quote: ... 60 µL CellTiter-Glo 3D reagent (Promega) was injected using the Tecan Spark Cyto Injector to each well ...
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bioRxiv - Microbiology 2024Quote: ... 60 µM CTP (Promega, 10 mM stock) and 2-4 µl 10 mCi.ml-1 of radiolabelled CTP [α-32P] ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
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bioRxiv - Biophysics 2022Quote: ... 60 sec] using the Go-Taq Flexi (Promega) thermostable DNA polymerase ...
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bioRxiv - Microbiology 2021Quote: ... 60 μL of One-Glo-EX substrate (Promega) were added to each well and incubated on a plate shaker in the dark ...
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bioRxiv - Genetics 2020Quote: ... containing 60 μM EnduRen Live Cell Substrate (Promega). After incubation for 4h at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... followed by addition of 60 units SmaI (Promega) plus 0.4 MBq [α32P] dCTP (222 TBq/mmol) ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested with 1 µg trypsin (Promega) and subjected to liquid chromatography (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Immunology 2022Quote: ... 60 μl/well of One-Glo-EX substrate (Promega) were added to the cells and incubated in the dark for 5-10 min prior reading on a Varioskan LUX plate reader (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2019Quote: ... 60 µl of CellTiter-Glo 2.0 (Promega, Madison, WI) reagent/well were added according to the manufacturer’s instructions and luminescence was quantified with a SpectraMax 190 plate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Physiology 2019Quote: ... Proteins were co-precipitated with 1 µg trypsin (Promega) overnight at −20 °C in 1 ml methanol ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with 1) chymotrypsin (Promega, Madison, USA), 2 ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.
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bioRxiv - Genetics 2024Quote: ... A 1:10 ratio (enzyme: protein) of Trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Developmental Biology 2021Quote: ... and 60 U/mL RNasin Plus RNase Inhibitor (Promega N2611). A 5% IGEPAL-630 solution was added to bring the homogenate to 0.3% IGEPAL CA-630 ...
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bioRxiv - Cell Biology 2022Quote: ... in 60% confluent HEK293T cells using FuGENE HD (Promega, E2311). 48 hours post transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... and lysed in 60 µl of Passive lysis buffer (Promega) at stage 12.5.
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bioRxiv - Immunology 2020Quote: ... and lysed in 60 μl of Passive Lysis Buffer (Promega). After 15 min of lysis ...
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bioRxiv - Genomics 2023Quote: ... and 60 fg unmethylated lambda-DNA (Promega, cat. no. D1521) at 37°C for 2.5 h.