Labshake search
Citations for Promega :
601 - 650 of 1320 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... or minimal promoter firefly luciferase reporter plasmid pGL4.26 [luc2/miniP/Hygro] (Promega, #E8441). Note ...
-
bioRxiv - Microbiology 2023Quote: ... The firefly luciferase expression plasmid pGL3-CMV was purchased from Promega (Madison, WI).
-
bioRxiv - Genomics 2023Quote: gBlocks with selected SNP-containing fragments (IDT) were cloned into pGL4.10 plasmid (Promega). For the luciferase reporter assay ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 1 µg of the specific lentiviral plasmid/construct using FugeneHD (Promega, E2311) in Optimem (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... The next day cells were transfected with plasmid DNA using Viafect (E4982, Promega) at a ratio of 3:1 viafect to DNA ...
-
Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... and envelop plasmid (VSVG) using FuGENE HD transfection reagent (Promega, Madison, WI, USA). The supernatant containing the constructed lentiviruses was collected after 24–48 h of transfection ...
-
bioRxiv - Microbiology 2020Quote: ... cell supernatants were analyzed by hemagglutination assay with rat erythrocytes26 and by Renilla luciferase assay (Dual-Luciferase® Reporter Assay System, Promega). Titers were calculated by the Spearman-Kaerber formula ...
-
bioRxiv - Developmental Biology 2024Quote: ... rat anti-Fat (kind gift from Helen McNeill) was used at 1:500 and mouse anti-β-galactosidase (Z3781, Promega; RRID:AB_430877) was used at 1:500 ...
-
bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
-
bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: Viral capped mRNA (rat HEV) was made from linearized DNA using the Promega Ribomax Large Scale RNA Production System SP6 (Promega PRP 1300) and ARCA CAP (TriLink Biotechnologies) ...
-
bioRxiv - Cell Biology 2020Quote: ... Cignal 45-pathway reporter arrays plasmids were transfected with ViaFect™ Transfection Reagent (Promega).
-
bioRxiv - Developmental Biology 2021Quote: ... Reporter plasmid (500 ng) and Renilla phRL-TK control vector (50 ng; Promega E2241) were cotransfected into HEK293T cells cultured in DMEM containing 10% FBS using Lipofectamine LTX Reagent with PLUS Reagent (Invitrogen 15338100) ...
-
bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA was isolated using Wizard® Plus SV Minipreps purification kit from Promega and used for yeast transformation by electroporation as described in (Benatuil et al. ...
-
bioRxiv - Immunology 2022Quote: ... 10 μg pΔ8.91 packaging and 8 μg lentiviral vector plasmid using Fugene (Promega, #E2312) according manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... was previously described (41) and the FFLuc expression plasmid (pGL4.13) was obtained from Promega. The pcDNA3.1(+ ...
-
bioRxiv - Microbiology 2019Quote: ... and the pool of transformants underwent plasmid preparation (miniprep) using a commercial kit (Promega). The miniprep was again digested with EcoRV and NotI and the resulting library was introduced into the conjugation helper strain ET12567-pUZ8002 (Hopwood et al. ...
-
bioRxiv - Microbiology 2019Quote: HPC229 plasmids were extracted using the Wizard DNA purification kit (Promega, Madison, WI, USA), and analyzed by 0.7% agarose gel electrophoresis and ethidium bromide staining following described procedures [13] ...
-
bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
-
bioRxiv - Synthetic Biology 2019Quote: ... Plasmid DNA was isolated using the Wizard Plus SV Minipreps DNA purification kit (Promega) or the NucleoBond Xtra Midi kit (Macherey-Nagel) ...
-
bioRxiv - Genomics 2019Quote: ... INS 832/13 cells were co-transfected with pGL3 promoter and psicheck plasmids (Promega) using lipofectamine 2000 (Thermofisher) ...
-
bioRxiv - Biochemistry 2019Quote: ... The plasmid was purified using Wizard® Plus SV Minipreps DNA Purification Systems (Promega). After checking the insert in the plasmid using PCR and electrophoresis ...
-
bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
-
Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... HEK293T cells were transiently transfected with a luciferase gene (nano-luc, plasmid pNL2.2, Promega) under the control of the IL-8 promoter [23] ...
-
bioRxiv - Bioengineering 2021Quote: ... pSFG.iCasp9.2A.14G2A-CD28-OX40-CD3ζ plasmid was MidiPrepped using the PureYield MidiPrep system (Promega). One day prior to transfection ...
-
bioRxiv - Bioengineering 2021Quote: ... VFC-CAR and VFC-Ctrl plasmids were MidiPrepped using the PureYield MidiPrep system (Promega). PCR amplicons were generated from plasmid templates using Q5 Hot Start Polymerase (NEB ...
-
bioRxiv - Bioengineering 2021Quote: ... The amount of plasmid was determined using Picogreen DNA assay (#E2670, Promega, Madison, WI) following the manufacturers’ instructions.
-
bioRxiv - Bioengineering 2021Quote: ... while the luciferase expression plasmid pGL4.50 was purchased from Promega (cat# E1310, Madison, WI).
-
bioRxiv - Neuroscience 2021Quote: ... All described plasmids were transfected into cells using FuGENE® HD Transfection Reagent (Promega) according to the manufacturers protocol ...
-
bioRxiv - Biophysics 2020Quote: ... mRNA was synthesized from these plasmids using a RiboMAX SP6 RNA production kit (Promega). In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmid DNA of insert-containing colonies was extracted (Wizard Plus SV Minipreps, Promega, France) and Sanger-sequenced in both directions by Eurofins Genomics (Germany) ...
-
bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
-
bioRxiv - Cell Biology 2021Quote: ... and a pcDNA5/FRT plasmid of interest using the Fugene 6 transfection kit (Promega) or Lipofectamine 2000 (Invitrogen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 50 pg of the pGL4.23 luc2/miniP enhancer:luciferase plasmid and the following amounts of MOs or mRNAs into each dorsal marginal zone (dmz ...
-
bioRxiv - Molecular Biology 2021Quote: The 3’ UTRs were cloned into psiCheck-2 plasmid (Promega, USA, Cat. No. C8021) using the XhoI and NotI sites ...
-
bioRxiv - Neuroscience 2021Quote: Wildtype and miR-544-3p binding site-mutated 3’UTR of Fcgr1 were purchased from Thermo Fisher Scientific (Waltham, MA) and the inserts subcloned into the psiCHECK2 plasmid (Promega, Madison, WI) using GeneArt seamless cloning and assembly enzyme mix (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293T TRIM21 KO cells were transfected with 2ug pGL4.32 NF-κB luciferase plasmid (Promega), using 12 uL of Viafect (Promega ...
-
bioRxiv - Genomics 2022Quote: ... were transfected with 0.5ug luciferase constructs and 50ng pGL4.73 [hRluc/SV40] plasmid (Promega, E6911) containing Renilla luciferase ...
-
bioRxiv - Developmental Biology 2022Quote: ... and/or pcDNA3.1-Flag-DN-xBrg1 plasmids using Fugen HD Transfection Reagents (2311; Promega), and after 48 h the transfected cells were lysed in NP-40 lysis buffer (P0013F ...
-
bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
-
bioRxiv - Neuroscience 2019Quote: ... or mCherry along with (ii) pGlosensorTM -22F cAMP biosensor plasmid (Promega Corp., Madison, WI), which encodes a modified form of firefly luciferase with a fused cAMP binding moiety providing a biosensor for the direct detection of cAMP signalling in live cells ...
-
bioRxiv - Biochemistry 2019Quote: ... and plasmids were extracted using the Wizard Plus SV Minipreps DNA Purification System (Promega). Plasmids were then treated with 5U DpnI (NEB ...
-
bioRxiv - Developmental Biology 2020Quote: ... For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega). For streptavidin pull-down assays from HEK293T cells ...
-
bioRxiv - Molecular Biology 2019Quote: ... The amount of DNA per well was equalized using mock plasmid DNA (pcDNA3.1, Promega). Forty-eight hours later ...
-
bioRxiv - Genetics 2021Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: PCR fragments were cloned into the pGEM-T Easy plasmid (Promega Corporation, Madison WI) under the conditions established by the manufacturers and E ...
-
bioRxiv - Biochemistry 2019Quote: ... and transfected with 0.5μg pCytERM-mAvicFP1 and pCytERM-mEGFP plasmid DNA using fuGENE (Promega) 24 hours prior to imaging ...
-
bioRxiv - Immunology 2020Quote: ... Transfection with plasmid was performed following standard protocols for Fu gene 6 (Promega, #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate ...
-
bioRxiv - Microbiology 2019Quote: ... and was cloned downstream of the Renilla luciferase gene in the pSICHECK2 plasmid (Promega) by XhoI and NotI restriction sites ...
-
bioRxiv - Genomics 2019Quote: ... The amplified sequence was inserted into nano-luciferase containing plasmid pNL3.2 (Promega, Madison, WI) using HindIII and XhoI (ThermoFisher ...