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Citations for Promega :
101 - 150 of 4129 citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... at a ratio of 11:18:1 using FuGENE HD transfection reagent (Promega). Forty-two hours after transfection ...
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bioRxiv - Genomics 2021Quote: ... and the protocol was completed according to manufacturer’s instructions (page 11, Promega #TM050).
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Immunology 2019Quote: ... Cytotoxicity was then measured from cell supernatants based on release of lactate dehydrogenase (LDH) released from dying cells based on the CytoTox 96® Non-Radioactive Cytotoxicity assay from Promega and done according to manufacture protocol ...
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bioRxiv - Neuroscience 2020Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
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bioRxiv - Immunology 2021Quote: ... culture supernatant was removed from each well and Beta-Glo® reagent (Promega, Madison, WI) was added and the chemiluminescence activity (Relative Light Units-RLU ...
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bioRxiv - Cancer Biology 2020Quote: ... 11 μl of CellTiter Glo (CellTiter-Glo Luminescent Cell Viability Assay, REF G7573, Promega) added ...
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bioRxiv - Neuroscience 2022Quote: The CytoTox 96 Non-Radioactive Cytotoxicity Assay or LDH-Glo Cytotoxicity Assay was performed as per manufacturer’s instructions using 50 uL conditioned media replicates to measure lactate dehydrogenase (LDH) release (Promega cat# G1780, J2380). For the CytoTox 96 assay 490 nm absorbance readings were taken on a SpectraMaxM5plate reader with SoftMax Pro 7.1 software and data is presented as 490 nm absorbance ...
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Cell Biology 2023Quote: ... Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423, Promega) via the following program ...
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bioRxiv - Microbiology 2020Quote: ... At the indicated time points growth media was removed and 10 μl Beta-Glo (Promega, Madison, Wisconsin) was added per well ...
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bioRxiv - Molecular Biology 2023Quote: ... Dual Luciferase assay kit and PsiCHECKTM-2 vector were purchased from Promega. Oligonucleotides were synthesized and obtained from Eurofins Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... we measured the release of lactate dehydrogenase (LDH) into the media effluents using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (G1780, Promega GmbH, Walldorf, Germany). The assay was performed in a 384-well plate according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cytotoxicity was evaluated by quantification of lactate dehydrogenase (LDH) release in culture supernatant of transfected-cells using CytoTox96 nonradioactive cytotoxicity assay (Promega, Charbonnières-les-Bains, France) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was evaluated by quantification of lactate dehydrogenase (LDH) release in cell cultures using CytoTox 96 nonradioactive cytotoxicity assay (Promega, Charbonnières-les-Bains, France) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... stored at 4°C and assayed the following day for lactate dehydrogenase (LDH) activity as a measure of cell death using a Cytotox 96® Non-radioactive Cytotoxicity Assay (Promega, Madison, Wisconsin, USA).
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... ELISA plates were read on a GloMax plate reader (Promega, Wisconsin, US). Data were converted from concentration to total mass based on the volume of media/pellet ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Genetics 2020Quote: ... Cell growth after 4 days was measured using the CellTiterGlo 2 kit (Promega) and a GloMax luminometer (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Plant Biology 2023Quote: ... The selected interactions were verified by quantitative β-galactosidase assays using Beta-Glo Assay System (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).
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bioRxiv - Molecular Biology 2021Quote: ... and the 82-nt exon 11 of the MAPT gene was constructed in the pCI-neo vector (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Genetics 2021Quote: ... Substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...
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bioRxiv - Cancer Biology 2019Quote: ... Luciferase and Renilla activities were measured by Biotek Synergy 2 plate reader (Winooski, VT) using the Dual-luciferase assay kit (Promega, Madison, WI).
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... 2-DG uptake was measured using a Glucose Uptake-Glo Assay kit (Promega, Madison, WI).
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2019Quote: ... Cell lysates were harvested the day after and luciferase and β-galactosidase activities were measured using Luciferase Reporter and Beta-Glo Assay Systems (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2021Quote: ... or experimental constructs along with the ptcΔ136-GL3 luciferase reporter construct and beta-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081).
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bioRxiv - Genomics 2021Quote: ... RNA concentration was measured in a DeNovix DS-11 spectrophotometer (DeNovix Inc, USA) and 4 μg of RNA were subjected to DNAse treatment (RQ1 - Promega) according manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Cell Biology 2020Quote: The sequence containing three consecutive perfect matches for hsa-miR-145-5p was excised from pSG5-Luc-3xmiR-145 (11) and sub-cloned in pMirGlo (Promega) to generate pMiRGlo 3xmiR-145.
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then digested with trypsin (11 μL of 0.5 μg/μL per sample, resuspended in trypsin resuspension buffer containing 20 mM CaCl2; Promega, #V542A) overnight at 37 °C in a shaker incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Immunology 2020Quote: ... Total IgG and ANA ELISA plates were read using a GlowMax Discover Microplate Reader (Promega) on the absorbance setting at 450nm.
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bioRxiv - Immunology 2022Quote: ... ELISA plates were immediately read using a GloMax®-Multi Detection System microplate reader (Promega) at 450nm absorbance.
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: ... Cytotoxicity was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay kit (Promega) by determining Formazan absorbance at 590 nm on a Tecan Safire automated plate reader ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized (using 2 µg RNA) using the Go Script™ Reverse Transcriptase Kit (Promega) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were successively lysed and assayed by a 2-DG uptake assay kit (J1341, Promega) according to the instruction ...