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Citations for Promega :
701 - 750 of 758 citations for Rabbit Anti Borrelia burgdorferi Polyclonal Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... washed thrice with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibody (Promega, Madison, WI, USA). After four additional washes ...
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bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
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bioRxiv - Cell Biology 2023Quote: ... chemiluminescent substrate or alkaline phosphatase (AP)-conjugated secondary antibody and Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega). Membranes were imaged using a ChemiDoc MP imaging system (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
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bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... DRG cultures were incubated with an antibody directed against the neuronal-specific marker βIII tubulin (Promega, Madison, WI, United States) diluted 1:2000 in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times for 15 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 3% BSA-TBST for 1h at room temp ...
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bioRxiv - Genetics 2021Quote: After the FISH protocol was complete gonads in PBS were incubated with primary antibodies diluted in PBS and 0.5 units/µl of RNasin (Promega N261A) at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... diluted in blocking buffer (1:10,000) was used as a secondary antibody and immunogenic bands were developed by the addition of BCIP/NBT substrate (Promega). The reaction was stopped with distilled water.
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed 3 times for 10 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 5% non-fat dry milk -TBST for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... 120 μg of fragmented total RNA was added to the antibody-bound beads in 250 μl IP buffer supplemented with RNasin Plus RNase inhibitor (PRN2615, Promega), and the mixture was incubated at 4 °C for 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... nitrocellulose membranes were blocked in 5% skimmed milk incubated with primary and secondary antibodies conjugated with alkaline phosphatase and developed with NBT/BCIP as substrates (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times for 15 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 3% BSA -TBST for 1h at room temp ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed twice with 1X PBS-T followed by incubation for 1 hour with HRP-conjugated secondary antibody (Promega) diluted 1:10,000 in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were probed with either goat anti-mouse IgG-HRP (Promega, catalogue no. W4021) or goat anti-rabbit IgG-HRP (catalogue no ...
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bioRxiv - Developmental Biology 2021Quote: ... and Dad13 constructs were detected with using mouse-anti-β-galactosidase (1:1000, Promega) and rabbit-anti-β-galactosidase (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... An anti-mouse IgG-horseradish peroxidase (HRP) conjugate (1:3,000 Promega, Fitchburg, United States) was used as secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... on embryos/larvae using mouse anti-β-gal (Promega catalog number Z3781, 1:1000) and rabbit anti-mCherry (BioVision ...
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bioRxiv - Immunology 2024Quote: ... The membrane was then washed thrice with 1X PBST and incubated for 1 hr with HRP conjugated secondary antibody (1:5000; Promega #w4018). After washing with PBST the membrane was developed using the enhanced chemiluminescence reagent (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated overnight at 4 °C with anti-p75NTR (Promega; G323A; 1:300) and anti-TRADD (Santa Cruz ...
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bioRxiv - Plant Biology 2021Quote: ... Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000) (Promega). Cy3-labeled and unlabeled dsDNA probes were generated with oligonucleotides listed in Supplemental Table 5 ...
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bioRxiv - Cell Biology 2020Quote: ... Cultures were then incubated overnight at 4°C with either anti-β3-tubulin (Promega #G712A) or anti-Oct4 (Santa Cruz Biotechnologies #SC-5279 ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were then incubated overnight at 4° C with anti-p75NTR (Promega; G323A; 1:500) and anti-TRAF6 (Santa Cruz ...
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bioRxiv - Neuroscience 2019Quote: ... Anti-sense RNA was then in vitro transcribed using a T3 RNA polymerase (Promega P2083) and a DIG RNA labeling mix (Roche 11277073910 ...
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bioRxiv - Neuroscience 2021Quote: ... Neurite outgrowth was visualized by immunostaining with mouse anti-β III tubulin (1:1000; Promega) followed by incubation with Alexa 594-conjugated anti-mouse secondary antibodies (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... Anti-proliferative effects were assessed by CellTiter-Glo Luminescent Cell Viability Assay (Promega cat #G7570) according to the manufacturer’s standards ...
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bioRxiv - Immunology 2023Quote: ... followed by goat anti-mouse immunoglobulin conjugated to alkaline phosphatase (Promega S3721, dilution 1:7500). Focuses of infection were revealed using NBT/BCIP reagent (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Molecular Biology 2022Quote: ... Afterwards wells were washed again with PBS-T thrice and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, WI, United States) was added in a dilution of 1 ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then subjected to immunoblotting and probed for anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG (Promega, Madison, WI, USA) as the secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Then wells were washed again three times with PBS-T (100 μl per well) and incubated with a secondary mouse-HRP antibody (goat, Promega, Madison, WI, USA) (50 μl per well ...
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bioRxiv - Molecular Biology 2022Quote: ... Subsequent to primary antibody removal chitin magnetic beads were washed three times with PBS-T and incubated with secondary mouse-HRP antibody (goat, Promega, Fitchburg, United States) 1:5000 in 100 μl sample buffer on a stirring wheel at room temperature for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with 10U ProTEV Plus (cat#: V6101, Promega) in the 1x ProTEV digestion buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... The anti-proliferative effects of these compounds were assessed using the Cell Titer Glo assay kit (Promega Cat. # G7570) following manufacturer protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Microbiology 2021Quote: The hydrolytic activity of immunoprecipitated PDEs bound to anti-HA magnetic beads was measured using the PDE-Glo assay (Promega), a bioluminsescence-based assay which quantifies the amount of cAMP or cGMP hydrolysed by a given PDE ...
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bioRxiv - Biochemistry 2024Quote: ... The binding of the Nbs to the coated protein was detected via their EPEA-tag using a 1:4000 CaptureSelectTM Biotin anti-C-tag conjugate (Thermo Fischer Scientific) in combination with 1:1000 Streptavidin Alkaline Phosphatase (Promega). Colour was developed by adding 100 µL of 3 mg/mL disodium 4-nitrophenyl phosphate solution (DNPP ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by a second round of TBST washes before incubation with HRP conjugated goat anti-mouse Ab (1:5000 Ab in 3 % BSA in TBST; Promega Corp.) for an hour ...