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Citations for Promega :
601 - 650 of 3880 citations for QuantiChrom Arginase Inhibitor Screening Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... was added to a final concentration of 3 mM followed by the addition of 5 µl of T7 Enzyme Mix and 50 U RNasin RNase Inhibitor (Promega). To produce transcripts for mock transfections the GTP concentration was increased to 7.5 mM and cap analogues were not added ...
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bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and 4 μl of this dilution was reverse transcribed using the imProm-II system with random hexamer primers and RNasin ribonuclease inhibitor (Promega). The generated cDNA was diluted with 300 ul nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s instructions and complementary cDNA synthesis was carried out using M-MLV reverse transcriptase in the presence of RNasin RNase inhibitor (Promega) and an oligo(dT ...
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bioRxiv - Biophysics 2019Quote: ... cells were treated with the LRRK2 inhibitor MLi-2 (100 nM) for 2 hours and then labelled with the TMR ligand (3 μM)(Promega). After 15 minutes ...
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bioRxiv - Physiology 2021Quote: ... and a 223-bp fragment of the inducible metalloproteinase inhibitor (IMPI) from the greater wax moth Galleria mellonella (AY330624.1) (dsIMPI) using the T7 RiboMAX™ Express RNAi System (Promega). In silico off-target prediction was done by searches of all possible 21-mers of both RNA strands against the local P ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Cell Biology 2021Quote: ... Beyotime) for 10 min at 37°C and the other was treated with 1 U/ul RNase inhibitor (N2518, Promega). For chromatin extraction ...
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bioRxiv - Microbiology 2022Quote: ... Huh7.5.1 cells were infected with MLB1 and MLB2 astroviruses (MOI 5) in the presence or absence of 20 µM z-VAD-fmk (pan-caspase inhibitor, Promega). At indicated time post infection ...
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bioRxiv - Neuroscience 2022Quote: ... The cell pellet was resuspended in ice cold PBS containing 0.04% bovine serum albumin (BSA) and 0.5 U/μl RNasin Plus RNase inhibitor (Promega, Madison, WI) to achieve a concentration of ∼750-1200 cells/μl ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of 600 ng was quantified using Nanodrop and converted to cDNA using MMLV Reverse Transcriptase with random primers and RNase inhibitor (Promega). RT-qPCR was performed using SYBR Green or TaqMan Universal PCR MasterMixes (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... a total of 225 μg of protein extract isolated using the aforementioned hypotonic buffer (additionally supplemented with 100 U/ml RNasin Plus RNase Inhibitor (Promega) and 2 U/mL Turbo DNase (Ambion) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Kinase activity of phosphorylated CDK2 with cyclin A and varying amounts of inhibitor (dinaciclib or 5) were measured using the ADP-Glo Kinase Assay (Promega) in a luminescence plate reader (Tecan Infinite M1000 PRO) ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... 120 μg of fragmented total RNA was added to the antibody-bound beads in 250 μl IP buffer supplemented with RNasin Plus RNase inhibitor (PRN2615, Promega), and the mixture was incubated at 4 °C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole chicken eyes were enucleated at E4 or E5 in PBS containing 0.2 U / µl RNasin Plus RNase Inhibitor (Promega, N2615). Eyes from 3 embryos were pooled for each condition ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were washed 3 times by resuspending in cold PBS containing 2% fraction V bovine serum albumin and 0.2 U / µl RNase inhibitor (Promega, N2615). For multiome assay ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100, 2 mM DTT, 100 μg ml-1 cycloheximide [Sigma], 20 U ml-1 RNase inhibitor [Promega] ...
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bioRxiv - Molecular Biology 2023Quote: ... and complementary DNA synthesis was carried out using M-MLV reverse transcriptase in the presence of RNasin RNase inhibitor (Promega). PCR primers are all intron spanning ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and pellet was resuspended in 400 µL of sort buffer (1 mM EDTA 0.2 U/µL RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using a commercial kit (Wizard Genomic DNA Purification Kit, Promega, Madison, WI). Libraries were produced by first fragmenting 75 ng DNA with the Nextera reagent (Illumina ...
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bioRxiv - Zoology 2024Quote: DNA was extracted with several different kits including the Wizard Genomic DNA Purification kit (Promega) and the Qiagen Blood & Tissue kit ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were centrifuged at 500 g for 5 min at 4°C and resuspended in 4 mL of nuclei wash buffer (PBS supplemented with 1% BSA and 0.2U/μL RNasin® Plus Ribonuclease Inhibitor (Promega, N2615). Following another round of centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5% sodium deoxycholate (protect from light), 1:200 Protease Inhibitor Cocktail III), and then, incubated with RNase I (Life Technology, AM2295) and DNase (Promega, M6101) in a Thermomixer at 1200 rpm to fragment RNA at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: Cell pellets were thawed in Lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP-40 supplemented with protease inhibitor cocktail (Promega cat #G6521)) and incubated on ice for 15min ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were thawed with lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% Triton X100, 1mg/ml Na-deoxycholate) supplemented with protease inhibitor (Promega cat #G6521). The bait is covalently bound through HaloTag to the beads and therefore cannot be detected by blotting.
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bioRxiv - Neuroscience 2020Quote: ... total RNA was placed in 4 µl of lysis buffer (0.1% vol/vol Triton X-100, 2.5 mM dNTPs, 2.5 µM oligo-dT, 1 U/µl Promega RNasin Plus RNase inhibitor). Reverse transcription was performed followed by cDNA amplification ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was added to the 100 µl SA Sepharose slurry together with 1 µl RNasin Plus RNase inhibitor (40 U/µL, Promega, N261A). 10 µl of the supernatant was saved for extraction of input RNA using TRIzol (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability of primary human nasal epithelial cells was analyzed during inhibitor only treatment at highest concentrations (50 uM, 60 uM) using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, G9241) and related to DMSO treated cells.
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bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug combinations were performed by treating cells with varying concentrations of the drugs/inhibitors (Table S1) and cell viability was measured by CellTiterGlo assay (Promega, G7572).
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Genomics 2021Quote: ... was extracted from tissue samples using a commercial kit (Wizard R Genomic DNA Purification Kit, Promega) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... pGEM®-T kit from Promega were used ...
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bioRxiv - Immunology 2019Quote: ... using a luciferase assay kit (Promega). Results were normalized to β-galactosidase activity (Galactostar ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: ... with the TNT kit (Promega: L1770). Each substrate was amplified with primers by PCR to allow T7-dependent transcription of the PCR product or transcribed directly if plasmids contained a T7 promoter ...
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bioRxiv - Microbiology 2019Quote: A Luciferase Assay System kit (Promega) was used ...
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bioRxiv - Plant Biology 2019Quote: The Reverse Transcription System Kit (Promega) was used to synthesize a cDNA library following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... the PureYield™ MidiPrep Kit (ProMega) and NucleoBond Xtra™ Midi/Maxi Kit (Macherey & Nagel ...
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bioRxiv - Genetics 2021Quote: ... using the Renilla Glo Kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... the Glutamate Glo Assay Kit (Promega) was used ...