Labshake search
Citations for Promega :
301 - 350 of 4809 citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... and digestion with 250 ng of LysC (Wako) or 250 ng of LysN (Promega) at 37°C for 16 h ...
-
bioRxiv - Microbiology 2020Quote: ... This was followed by overnight digestion in 10 mM ammonium bicarbonate in trypsin (Promega) at 37°C ...
-
bioRxiv - Physiology 2020Quote: ... and in-gel tryptic digestion was carried out using ProteaseMAX reagent (Promega, WI, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... Trypsin digestion was performed overnight with sequencing grade modified Trypsin (Promega, Madison, Wisconsin, USA). Peptides were extracted with 100% ACN and 50% ACN/ 5 % HCOOH ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteomic digestion was performed by the addition of 1 μg proteomics-grade Trypsin (Promega) and incubated overnight at 37 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight trypsin digestion (ratio 1:33 enzyme:lysate, Promega, Madison, WI, USA).
-
bioRxiv - Systems Biology 2021Quote: ... 100-200 ng of purified digestion was ligated by T4 DNA ligase (Promega M1801), and 2 μL of the product were transferred into 40 μL of TOPO competent cells (ice incubation for 20 min ...
-
bioRxiv - Plant Biology 2020Quote: ... Genomic DNA was eliminated by on-column digestion with RQ1 RNase-free DNase (Promega) during the isolation procedure ...
-
bioRxiv - Microbiology 2021Quote: ... and gel bands excised before in-gel digestion using modified trypsin (Promega, sequencing grade). Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
-
bioRxiv - Microbiology 2020Quote: ... Afterwards 40 µL digestion buffer (1:10 diluted Rapid Digest buffer (Promega, Fitchburg, WI) in water ...
-
bioRxiv - Cell Biology 2022Quote: Gel bands were subjected to in-gel digestion with sequencing-grade modified trypsin (Promega) as detailed in Belluzzi et al. ...
-
bioRxiv - Microbiology 2022Quote: ... Digestion was performed with trypsin in a ratio of 1:20 m:m protein:trypsin (Promega, P/N V5072 for the pilot study ...
-
bioRxiv - Plant Biology 2021Quote: ... and digestion with 12.5 ng/μl modified sequencing grade porcine trypsin (Promega, Madison, WI) at 45°C for 1 hour ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tryptic digestion was performed overnight with 0.3 µg trypsin (Promega Sequencing Grade; Madison, WI) per sample ...
-
bioRxiv - Immunology 2022Quote: ... The proteolytic digestion was continued by the addition of 500 ng of trypsin (Promega) and incubating overnight at 37°C with gentle shaking ...
-
bioRxiv - Biochemistry 2019Quote: ... Enzymatic digestion was performed by addition of 1 μg trypsin (Gold, Promega, Madison USA) in TEAB 100 mM and incubation overnight at 30°C ...
-
bioRxiv - Microbiology 2020Quote: ... Digestion was performed by adding 10 μL of 0.2 mg/mL Trypsin Gold (Promega) and overnight incubation at 37°C ...
-
bioRxiv - Bioengineering 2020Quote: ... Peptides were then produced by trypsin digestion (50 ng trypsin, Promega Sequence Grade, Modified) (Liu et al ...
-
bioRxiv - Genomics 2021Quote: ... DNA collected from the DNase I digestion was quantified using a Quantus Fluorometer (Promega), and 10 ng was used to generate the libraries ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA was removed by digestion with RQ1 RNase-Free DNase (Promega, Madison, WI) and RNA converted to cDNA using the ThermoScript RT-PCR system (ThermoFisher) ...
-
bioRxiv - Cell Biology 2022Quote: ... Digestion buffer with 2.5 µg of sequencing grade Trypsin and ProteinaseMax (Promega, V5111, V2071) was loaded on the column and incubated at 37°C overnight ...
-
bioRxiv - Plant Biology 2022Quote: ... The digestion solution was diluted to <1 M urea upon addition of trypsin (Promega, V511A ...
-
bioRxiv - Systems Biology 2023Quote: ... washing, and digestion with lysyl endopeptidase (Lys-C, Wako) and sequencing-grade trypsin (Promega). The capture and digestion steps were performed with a Versette liquid handling robot (ThermoFisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
-
bioRxiv - Biochemistry 2023Quote: Tryptic digestion was performed at 37° C with 125 μL Trypsin (Promega Cat# V511X) in digestion buffer (100 mM TEAB pH 8.5 ...
-
bioRxiv - Microbiology 2023Quote: ... followed by digestion with benzonase at 37 °C for 1 h and trypsin (Promega) digestion overnight at the same temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... Parental plasmids were selectively linearised from the PCR product via digestion with Dpnl (Promega) in lx MULTI-CORE buffer for 1hr at 37°C and the digestion product was immediately transformed into Stbl3 competent bacteria.
-
bioRxiv - Biochemistry 2023Quote: ... The latter was subjected to an additional digestion with 100 ng of trypsin (Promega) for 6 hours at 37 °C.
-
bioRxiv - Cell Biology 2023Quote: ... Subsequent digestion was performed with Trypsin/LysC solution [100ng/μl 1:1 Trypsin (Promega):LysC (FujiFilm ...
-
bioRxiv - Plant Biology 2023Quote: ... Samples were digested in fresh digestion buffer containing 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega ...
-
bioRxiv - Genomics 2024Quote: ... Trypsin digestion was done by adding 5 µL reductively methylated trypsin (Promega, Madison, USA) to each sample and incubating the samples overnight at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
-
bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
-
bioRxiv - Biophysics 2021Quote: Some protein kinase assays were performed using the ADP-Glo™ assay kit (Promega), which measures the generation of ADP in a kinase reaction ...
-
bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
-
bioRxiv - Plant Biology 2022Quote: ... DNA digestion was performed using the RQ1 RNase-free DNase set (Cat No.: M6101, Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2019Quote: ... Digestion was performed for ∼18 hours at 37 °C using sequencing grade Glu-C (Promega). Peptides were analysed by nanoHPLC-MS/MS over a 65 min acquisition with elution from a 50 cm C18 PepMap column onto an Orbitrap Fusion Tribrid mass spectrometer via an Easy-Nano ionisation source ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a 4 times dilution in Tris buffer and an overnight trypsin digestion (Promega) at a ratio 1/100 ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was submitted to DNA digestion with RQ1 RNase-free DNase (Promega, Fitchburg, WI, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... IAA excess quenched by additional DTT) and trypsin digestion (750ng of sequencing grade trypsin, Promega) in 50mM NaHCO3 buffer ...
-
bioRxiv - Developmental Biology 2022Quote: ... a second round of DNase digestion was added using the RQ1 RNase-free DNAse (Promega), in order to remove any putative residual DNA ...
-
bioRxiv - Developmental Biology 2022Quote: ... The samples were subjected to digestion with LysC (Wako) for 2 h and trypsin (Promega) overnight at 37°C at a 1:50 enzyme ...
-
bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
-
bioRxiv - Biochemistry 2020Quote: ... Digestion of each sample was performed using 1 μg of Sequencing Grade Modified Trypsin (Promega) in TEAB per sample at 47 °C for 1 h ...
-
bioRxiv - Immunology 2022Quote: ... Proteolytic digestion was initiated by the addition of 250 ng of endoproteinase Lys-C (Promega) and incubating at room temperature for 2 hours ...
-
bioRxiv - Cell Biology 2019Quote: On bead digestion was performed by adding 1 μg of MS-grade trypsin (Promega, V5280) to each tube and incubating at 37 °C with shaking overnight ...
-
bioRxiv - Paleontology 2020Quote: ... 100 μL microliters of digestion buffer and 40 μl of trypsin / LysC (Promega, Madison, USA) were added and incubated with shaking in a heating block tube (MHR23 ...
-
bioRxiv - Systems Biology 2019Quote: ... samples were diluted with 4 volumes of Digestion Buffer (50 mM NH4HCO3) and trypsin (Promega) was added at a ratio of 1:50 ...
-
bioRxiv - Systems Biology 2019Quote: ... samples were diluted with 4 volumes of Digestion Buffer (50 mM NH4HCO3) and trypsin (Promega) was added at a ratio of 1:50 ...
-
bioRxiv - Developmental Biology 2021Quote: ... a second overnight digestion step using 200 ng modified porcine trypsin (Promega, Madison, WI, USA) was performed at 37°C ...