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Citations for Promega :
401 - 450 of 4594 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... then adding 30 μl of pre-mixed nGlo buffer with the recommended substrate concentration (Promega, #N1110). Luminescence was read in black opaque 96 well plates (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with 1 μg/μL of RNA concentration using MMLV-RT enzyme (Promega, USA). HvHsfA6a gene specific and Actin primers designed by using Primer express 3.0 software (Thermoscientific USA ...
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bioRxiv - Cell Biology 2023Quote: ... proteinase K digestions were stopped by 10 mM phenylmethanesulfonyl fluoride (final concentration) (Protease Inhibitor Cocktail, Promega) on ice for 20 min ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) using 2 μg Plasmid DNA per 50 μL expression reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Cell Biology 2020Quote: ... Germany) prior to imaging with a final concentration of 31 pM according to the HaloTag protocol (Promega). For binding time measurements ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products with low concentration or bad sequencing quality were cloned into pGEM-T Easy Vector (Promega) for sequencing ...
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bioRxiv - Biophysics 2020Quote: ... Changes in the intracellular cAMP concentration of the HEK293 cells were measured by the GloSensor assay (Promega). The transfected cells were incubated with or without 0.5 μM all-trans-retinal (Toronto Research Chemicals) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8 μl 100% DMSO, 2.5 μl T4 RNA ligase I – high concentration [NEB], 0.4 μl RNasin [Promega] ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with different concentrations of TMR HaloTag ligand according to the HaloTag labeling protocol (Promega) and 50,000 cells were analyzed by flow cytometry (BDLSR Fortessa ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of PCR products was then measured using the QuantiFluor™ dsDNA System (Promega, Madison, WI) and used to pool equimolar amounts of PCR products together ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic DNA concentration was determined using a Quantus Fluorometer with QuantiFluor dsDNA System (Promega, Madison, WI, USA), and 1 g/mL streptomycin input DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Experiments with varied activator concentration and screening were measured using the Kinase-Glo Luminescent Kinase Assay (Promega). Reactions were incubated at 25°C for 1 hour then reduced to 4°C in a thermocycler consisting of 50nM of recombinant VCP with or without 50nM UFD1/NPLOC4 in 50uL ATPase reaction buffer (25mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The concentration at which 50% of cell growth was inhibited (GI50) was determined using Cell TiterGlo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and dNTP mix at a final concentration of 0.5 mM of each dNTP (Cat. No. U1511; Promega). Semi-quantitative RT–PCR amplifications were performed on 1 μl of cDNA in a 12.5-μl reaction volume to start with ...
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bioRxiv - Physiology 2023Quote: ... primers at a final concentration of 300nM and 6µL of GoTaq® qPCR Mix (Promega, Madison, USA). qPCR conditions were the following ...
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bioRxiv - Cell Biology 2023Quote: ... The NO concentration in the culture medium was measured using the Griess reagent system (Promega, Tokyo, Japan). To determine the direct effects of mannosidase on the matrix ...
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bioRxiv - Plant Biology 2024Quote: ... Amplicon concentration was quantified with a ND 1000 UV–vis spectrophotometer (NanoDrop Technologies) and Quantus (Promega, USA).
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bioRxiv - Microbiology 2021Quote: ... DENV2 structural proteins were cloned into expression vector from Promega in frame with a C-terminal Flag tag ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins/peptides were further digested with trypsin (Trypsin Gold, Promega) at 37 °C overnight ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Proteins were digested using MS grade trypsin (Promega Trypsin Gold) in a ratio of 1:50 for 12 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested with 6 ng/µl trypsin (Promega, UK) overnight at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were digested using sepharose bead-immobilized porcine trypsin (Promega) at a ratio of 50 ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted protein was first treated with PNGase-F (Promega) to remove the N-linked glycosylation and then analyzed by mass spectrometry to determine the N-terminal sequence ...
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bioRxiv - Biochemistry 2019Quote: ... Tryptic digestion: the proteins were digested with trypsin (Promega, USA) using filter-aided sample preparation methods[25] ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega).
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Microbiology 2019Quote: ... Protein digestion was performed with 1.5 µg of Trypsin (Promega) overnight at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins were enzymatically digested using sequencing grade modified trypsin (Promega) overnight at 37ºC ...
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bioRxiv - Plant Biology 2022Quote: ... The proteins were enzymatically digested using LysC/Trypsin Mix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested with 20:1 protein:trypsin (Trypsin Gold, Promega) in 50 mM ammonium bicarbonate for 3 hours at 47 °C before adding an additional 1 µg of trypsin and digesting for a further 1 hour at 47 °C ...
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bioRxiv - Cell Biology 2022Quote: Proteins retained in the resin were digested with trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein digestion was performed by adding trypsin (Promega Biotech AB) 1:50 (enzyme:protein ratio ...
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bioRxiv - Biochemistry 2021Quote: ... and the S30 T7 High-Yield Protein Expression System (Promega) were used to express proton-coupled folate transporter (PCFT ...
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bioRxiv - Immunology 2020Quote: ... the protein suspension was digested with 3 μg trypsin (Promega) in 40 μl 25 mM NH4HCO3 overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... and proteins were visualized with a chemiluminescence detection system (Promega). Band intensities were quantified using AlphaView Software and results are expressed relative to the control condition ...
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bioRxiv - Microbiology 2022Quote: ... enriched proteins were digested on-bead with trypsin (Promega, V5111). Peptides were acidified with 0.5% (v/v ...
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bioRxiv - Systems Biology 2022Quote: ... the precipitated protein samples were digested with Trypsin/LysC (Promega) (1:50 wt/wt. ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were expressed in BL21 (DE3) pLysS cells (Promega) induced with 0.5 mM isopropyl-1-thio β-D-galactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2021Quote: ... the protein suspensions were digested with 4 μg trypsin (Promega) in 40 μl TEAB buffer overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: Proteins bands were excised and treated with Proteinase K (Promega) and trypsin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then digested with 1:50 trypsin-protein (Promega) at 37°C overnight in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts were digested with sequencing-grade trypsin (Promega®) as previously described (Chicher et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...