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Citations for Promega :
401 - 450 of 1665 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, USA) at an enzyme-to-protein ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with sequencing-grade trypsin (Promega) at room temperature while shaking overnight ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were digested with 0.3 µg LysC (Promega) for 16 h at 37°C followed by a second digestion step with 0.15 µg trypsin (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were digested with 1 µg trypsin (Promega) and subjected to liquid chromatography (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Ingel protein digestion using trypsin (Promega, Mannheim, Germany) was performed essentially as described before62 ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... AccuMAP™ Low pH Protein Digestion Kit (Promega) according to the manufactures protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were deglycosylated by Endo-H (Promega) followed by PNGaseF (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with trypsin (sequencing grade, Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... precipitated proteins were first treated with trypsin (Promega) in 50 mM ammonium bicarbonate solution ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were then digested with modified trypsin (Promega) at an enzyme/substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Physiology 2023Quote: ... Proteins were digested using sequencing grade trypsin (Promega) at a 1:40 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Add 200 μl of Protein Precipitation Solution (Promega) and mix by pipetting ...
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bioRxiv - Neuroscience 2023Quote: ... Purified HaloTag protein (#G4491) was purchased from Promega. Pluronic F-127 (# P3000MP) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were digested with Lys-C/Trypsin (Promega) at 37 °C overnight with rotation ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were digested with trypsin (Promega, Wisconsin, USA) in a ratio of 1:100 at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... Proteins were on-bead digested with trypsin (Promega) at 37 °C for 16 h with orbital shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Protein precipitations were digested with Trypsin (Promega, #V5113) diluted in Digestion buffer (100 mM Ammonium Bicarbonate and 10% Acetonitrile ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplicons were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and sequenced via the Eurofins TubeSeq service ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using the Wizard SV Gel and PCR Clean-up System (Promega, A9281) as per manufacturer directions ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Cleanup System (Promega). Plasmid DNA was isolated from bacterial cultures with the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Genomics 2019Quote: ... the gel slices were then hydrated with 5 ng/uL sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate and digested overnight at 37°C on an orbital shaker ...
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bioRxiv - Developmental Biology 2020Quote: ... The gel pieces were hydrated and incubated with 2.5 ng/μl of trypsin (Promega) in 50 mM ammonium bicarbonate (ABC ...
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bioRxiv - Physiology 2020Quote: ... and in-gel tryptic digestion was carried out using ProteaseMAX reagent (Promega, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... purified and concentrated using Wizard® SV Gel and PCR Clean-Up System (Promega). Sanger sequencing confirmed correct amplification of PCR fragments ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was cut into 3 slices and in-gel digested with trypsin (Promega) as previously described15 ...
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bioRxiv - Neuroscience 2021Quote: ... Resulting products were purified using Wizard SV Gel and PCR Clean-Up columns (Promega) and submitted for Sanger and NGS (Amplicon EZ ...
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bioRxiv - Neuroscience 2020Quote: ... Single bands were excised according to their predicted fragment size and gel-purified (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Microbiology 2021Quote: ... Products were cleaned with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned with the pGEM-T easy Vector Systems (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... which was then purified using the Wizard Gel and PCR Clean-Up System (Promega) and submitted to a third party for sequencing.
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bioRxiv - Cell Biology 2019Quote: ... gel purified-PCR products were ligated into NheI/NcoI-digested pGL3-basic vector (Promega) immediately upstream of the luciferase start codon ...
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bioRxiv - Microbiology 2019Quote: ... we performed Pulse Field Gel Electrophoresis (PFGE) on S1 nuclease (Promega, Madison, WI, USA) digested DNA ...
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bioRxiv - Cell Biology 2021Quote: ... and gel bands were manually excised and digested with sequencing-grade trypsin (Promega, USA). The digested peptides were analyzed using a QExactive mass spectrometer (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in vitro expressed DBDs (see above) and the Gel Shift Assay Core System (Promega). Double-stranded DNA probes were generated by annealing sense and antisense oligonucleotides (see Table S-M1a ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel-purified fragment was cloned into the p-GEM-T Easy vector (Promega) and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: ... PCR bands were extracted using Wizard SV Gel and PCR Clean-Up System (Promega) and ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were then purified (Promega Wizard SV Gel and PCR Clean-Up System) and sequenced on both strands (Eurofins Genomics ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction using the Wizard SV gel and PCR clean-up system (Promega). The purified candidate genes were cloned into pCR8/GW/TOPO entry vectors (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... gel pieces were destained and then incubated with 10 ng/μl trypsin (Promega, #V5111) overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: After PCR clean-up using Wizard SV Gel and PCR Clean-Up (Promega; A9281), samples were run in 2% agarose gel to ensure that the PCR product is compose of a single amplicon in the appropriate size ...
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bioRxiv - Microbiology 2021Quote: ... DNA was purified with the Wizard® SV Gel and PCR Clean system (Promega). The DNA concentration of amplicon was measured by NanoDrop (ThermoFisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... in-gel purified and ligated into the pGEM-T Easy vector (Promega, Cat. A1360) and submitted to Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... were purified using Wizard® SV Gel and PCR Clean-Up System (Promega, USA), and then directly sequenced in both directions by Macrogen Inc ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were rehydrated in 60 μL of 20 μg/mL Trypsin (Promega) in 50 mM ammonium bicarbonate solution and incubated for 2h at 55°C ...
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bioRxiv - Molecular Biology 2022Quote: ... SYBR Green I nucleic acid gel stain and Go Taq Hot Start Polymerase (Promega). qRT-PCRs were carried out in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... and Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) was used to recover the desired bands from the agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... PCR samples were purified by the Wizard SV Gel and PCR Clean system (Promega) following manufacturers’ instructions ...