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Citations for Promega :
201 - 250 of 904 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Total RNA from colon tissues was isolated using Maxwell® 16 LEV simplyRNA tissue kits (Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from embryo composite samples or adult tissue using ReliaPrep RNA Tissue Miniprep System (Promega). Reverse transcription was performed on RNA to produce cDNA for RT-PCR using an iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... the Maxwell RSC simplyRNA Tissue (Promega, #AS1340) and the Macherey-Nagel Mini kit Nucleospin protocol (Macherey-Nagel ...
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bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
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bioRxiv - Neuroscience 2021Quote: mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega), as previously described (23 ...
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bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
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bioRxiv - Biochemistry 2020Quote: ... Lep variants were then translated using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... reactions were prepared as per Rabbit Reticulocyte Lysate System (Promega Cat#L4960). 10 µL of reaction mixture was supplemented with 1 µg of either Renilla or Firefly luciferase mRNA alongside recombinant GST or GST-PKM1 ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) (Promega). Typical reactions were composed of 90% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: Lysates for luciferase assay were prepared in 1× passive lysis buffer (Promega), 100 μl per well of a 24-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 μl of lysate was incubated with 50 μl luciferase reagent (Promega) and measured for 10 s using Lumat LB9507 (EG&G Berthold) ...
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bioRxiv - Microbiology 2022Quote: ... and a dual luciferase detection assay was conducted upon the lysates (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity in the lysates was analyzed using Luciferase Assay System (Promega) as per manufacturer’s instructions and as conducted previously (Vasudevan and Steitz ...
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bioRxiv - Cell Biology 2023Quote: ... 10 μl of lysate was incubated with 50 μl luciferase reagent (Promega) and measured for 10 s using (Lumat LB9507 ...
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bioRxiv - Cell Biology 2023Quote: Lysates for luciferase assay were prepared in 1× passive lysis buffer (Promega), 100 μl per well of a 24-well plate ...
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bioRxiv - Cell Biology 2023Quote: Lysates were digested from the beads with 100 ng of LysC (Promega) in 25mM HEPES pH 8.5 overnight at 37 °C with shaking at 1200 RPM in a thermomixer ...
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bioRxiv - Molecular Biology 2023Quote: ... and lysates were assayed for luciferase activity using Bright-Glo™ (Promega) on a 96-well using The VICTOR3™ Multilabel Plate Reader (PerkinElmer LAS Germany GmbH).
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bioRxiv - Synthetic Biology 2023Quote: ... cell lysates were harvested with 1X Passive Lysis Buffer (5X, Promega #E1941) diluted in water ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then analyzed by the addition of luciferase reagent LAR (Promega) in white microtiter plates using a Tecan plate reader and represented as relative light units (RLU ...
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bioRxiv - Cancer Biology 2024Quote: ... Clarified lysates were quantified using the Pierce TM BCA protein assay (Promega) and boiled in 1x sample buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells or murine liver tissue using the Relia Prep RNA Tissue Miniprep System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from whole mount larvae or dissected tissue using the Maxwell RSC simplyRNA Tissue kit (Promega Corp. ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from tissues (epididymis, lumbal DRGs and liver) using Relia prep™ RNA Tissue Miniprep System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The total RNA of tissues was extracted using the Maxwell RSC simply RNA tissue kit (AS1340, Promega, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from about 35 mg of tissue using the Maxwell™ 16 Tissue DNA purification kit (Promega) and total DNA concentrations were normalized to 20 ng/µl ...
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bioRxiv - Plant Biology 2022Quote: ... All samples were ground in liquid nitrogen and RNA was extracted using RNA isolation protocol for non-fiberous tissue by the RNA Tissue Miniprep System (Promega). cDNA synthesis was done using 1µg of total RNA with the qScriptTM cDNA Supermix kit (Quanta BioSciences) ...
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... Clear lysates were treated with 5U RQ1 DNase and 5U RNase inhibitor (Promega) at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were sequentially treated with 12.6 μl of RNase-free DNase (Promega) and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units ...
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bioRxiv - Microbiology 2020Quote: ... 15µl of lysate were mixed with 50µl of Nano-Glo assay buffer (Promega) in a flat bottom white-walled luminescence plate ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were analyzed for luciferase activity using GloMax Discover Microplate Reader (Promega). For wild-type SARS-CoV-2 infection ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were collected and analysed using the dual-luciferase assay kit (Promega) following the manufacter’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were collected 24 hours post-transfection using Passive lysis buffer (Promega). Samples from three technical replicate transfections were measured in duplicate using a Zylux FB12 luminometer and the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... Epe1 fragments were translated in vitro using TNT T7-coupled reticulocyte lysate (Promega) with 35S-labeled methionine (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were analysed using the Dual-Luciferase Reporter Assay System (Promega, #E1910). Luminiscence was measured using a TECAN infinite M200-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysates were subjected to luciferase activity testing (Promega, Madison, WI, USA) 24 h later ...
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bioRxiv - Immunology 2021Quote: PP2A activity in cell lysates was measured using the Phosphatase Assay Kit (Promega). Endogenous free phosphate was removed using the columns ...
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bioRxiv - Developmental Biology 2021Quote: ... lysates were simultaneously analyzed for firefly luciferase activity and Renilla luciferase activity (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Lysate samples are generated by treatment of 1x Passive Lysis Buffer (Promega E1941) to 12 well plate cultures ...
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bioRxiv - Microbiology 2022Quote: ... Seventy five ug of each lysate were digested with sequencing-grade trypsin (Promega) 1:100 ratio for 12 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
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bioRxiv - Systems Biology 2022Quote: ... All lysates were further purified in the Maxwell RSC instrument (Promega, Madison, USA), using the Maxwell RSC blood DNA kit (n=376) ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation assays were performed using nuclease-treated rabbit reticulocyte lysate (Promega). Reactions were in 10 µl containing 2 µl RRL ...
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bioRxiv - Immunology 2023Quote: ... cell lysates were analyzed using the Dual-Luciferase® Reporter Assay System (Promega) following the manufacturer’s instructions ...