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Citations for Promega :
1 - 50 of 2903 citations for Platelet endothelial aggregation receptor 1 PEAR1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected with a 1:1 ratio of receptor construct and GloSensor22F cAMP biosensor (Promega) plasmids.
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... and M75F receptor plasmids using FuGene HD (Promega). Cells were treated with DMSO or varying drug concentrations 24 hours after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR was used to amplify the coding regions of the steroid receptor genes and to linearize the expression plasmids pHTN HaloTag CMV Neo or pFLN-1 NanoLuc (Promega #N1811 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Ligand-induced β-arrestin recruitment to receptors was monitored by Nanoluciferase complementation assay (NanoBiT, Promega). Briefly ...
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bioRxiv - Biochemistry 2022Quote: HEK293 T cells were co-transfected with the target receptor construct and GloSensor cAMP reporter (Promega) in DMEM supplemented with 10% FBS for overnight incubation ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Biochemistry 2024Quote: lrl 1μg of N-terminally FLAG-tagged receptor and 1μg of F22 (Promega, Cat. no: E2301) (for GloSensor assay)
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bioRxiv - Biochemistry 2024Quote: ... surface and total receptor expression were determined using the Nano-Glo HiBiT extracellular detection system (Promega) and Nano-Glo HiBiT lytic detection system (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
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bioRxiv - Biophysics 2020Quote: ... antibodies followed by a 1-hour incubation with the corresponding HRP-conjugated secondary antibodies (1:2000, Promega) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used are anti-HaloTag monoclonal antibody (Promega, G921A, 1:1000), and polyclonal rabbit antibodies ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies used: ßgal (1:100, Promega), pMad (1:800 ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Ligand-induced β-arrestin recruitment to receptors was measured with a GloMax Discover plate reader (Promega, USA) for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293 cells were transfected with FLAG-tagged receptor (3.5 μg) and luciferase-based 22F cAMP biosensor construct (3.5 μg) (Promega). 14–16 h post transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: To measure ATP bound to detergent purified P2X1 receptor a Kinase-Glo® Luminescent Kinase Assay (ProMega, USA) was used ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (1.764 µg) were also transfected into HEK293T cells cultured on acid-washed coverslips using FuGENE HD (Promega) in Optimem (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... HEK293T cells were co-transfected with FLAG-tagged receptor (3.5μg) and luciferase-based 22F cAMP biosensor construct (3.5μg) (Promega, Cat. no: E2301) using polyethyleneimine (PEI ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Secondary antibody (donkey α-goat HRP, Promega, 1:10,000). For His-tagged proteins ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti- mouse IgG antibody (1:1,000, #S3721, Promega).
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies: anti-mouse-IgG (Promega, W4028, 1:2500) and anti-rabbit-IgG (Promega ...
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bioRxiv - Biophysics 2021Quote: ... sapiens TACAN-eGFP or the M2 muscarinic receptor (Halo-M2R-eGFP) plasmid (previously established in this lab) was transfected into the cells using FugeneHD (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... GRK371 and β-arrestin272 recruitment to chemokine receptors (CXCR1, CXCR2, CXCR3-A, CXCR4, CXCR5, CXCR6 and ACKR3) was monitored using a nanoluciferase complementation-based assay (NanoBiT, Promega)73,74 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Neuroscience 2023Quote: Surface expression was measured using a HiBit-tagged 5-HT2A receptor and the Nano-Glo HiBit Extracellular Detection System (Promega). N-terminal HiBit-tagged human 5-HT2A receptor was cloned into pcDNA3.1 using Gibson Assembly ...
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bioRxiv - Cell Biology 2023Quote: Protein-protein interactions between Mkl1/2 and Foxo1/3/4 or glucocorticoid receptors were also measured using NanoBiT PPI Starter Systems (Promega). 293FT cells were seeded in 96-well white wall microplates and co-transfected with the LgBiT and SmBiT plasmids ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with a secondary antibody (Gpx4 1:10000 or 4HNE 1:10000, W4011, Promega and Gapdh 1:10000 or ActB 1:5000, W4021, Promega), diluted in 10% TBS blocking buffer (Licor ...
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bioRxiv - Developmental Biology 2021Quote: ... and HRP-labelled secondary antibodies (RRID:AB_430833; RRID:AB_430834; Promega; 1:2,000) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega). Chemiluminescent signals were detected and digitally analyzed ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies include anti-NLuc rabbit polyclonal (1:6,000) (Promega), anti-mCherry rabbit polyclonal (1:5,000 ...
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bioRxiv - Immunology 2022Quote: ... HRP conjugated secondary antibodies were purchased from Promega (1:5000) or Novus (1:5000).
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bioRxiv - Cell Biology 2022Quote: ... Anti-Halo antibody was purchased from Promega (G9211; 1:1000). Anti-actin-HRP antibody (C4 ...
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bioRxiv - Neuroscience 2024Quote: Anti-HaloTag primary antibody (rabbit, 1 mg/ml, dilution 1:500; catalog no. G928A, Promega) and iFluor 647-conjugated anti-rabbit secondary antibody (goat ...
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bioRxiv - Molecular Biology 2024Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies were 1:5,000 HRP-conjugated anti-rabbit (W4011; Promega) or mouse (W4021 ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence utilizing primary antibodies directed against βIII-tubulin (1:1000, Promega) and secondary antibodies Alexa Fluor-488 (1:500 ...